LOCUS       PPV325-pAAV-Ef1a-DIO-FLPo-Myc      6932 bp    DNA     circular UNA 21-Feb-2013
DEFINITION  
COMMENT     Source: pAAV-Ef1a-DIO-hChR2(E123T/T159C)-mCherry-(Addgene 35510)
FEATURES             Location/Qualifiers
     Misc._feature   1..141
                     /label="L-ITR (AAV-2)"
     promoter        156..1419
                     /label="Ef1alpha promoter"
     Region          1450..1483
                     /label="lox2722"
     primer          complement(1479..1498)
                     /note="Oligo predicted by CLC primer designer"
                     /note="Oligo type: primer"
                     /note="Melting temp.: 55.75"
                     /label="P0373s"
     primer          1486..1504
                     /note="Oligo predicted by CLC primer designer"
                     /note="Oligo type: primer"
                     /note="Melting temp.: 55.48"
                     /label="P0371s"
     Region          1534..1567
                     /label="loxP"
     CDS             complement(1576..2904)
                     /cds_type="ORF"
                     /note="Length: 1329"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
                     /label="FLPo-Myc"
     misc_feature    complement(1579..1608)
                     /label="Myc-tag"
     primer          complement(1751..1769)
                     /label="P0405s"
     primer          2679..2696
                     /label="P0404s"
     Region          complement(2917..2950)
                     /label="Lox2722"
     primer          complement(2991..3009)
                     /note="Oligo predicted by CLC primer designer"
                     /note="Oligo type: primer"
                     /note="Melting temp.: 53.74"
                     /label="P0372s"
     Region          complement(3001..3034)
                     /label="LoxP"
     Misc._feature   3049..3657
                     /label="WPRE"
     primer          3224..3241
                     /label="qWPREfor"
     primer          complement(3340..3358)
                     /label="qWPREtp"
     primer          complement(3454..3471)
                     /label="qWPRErev"
     polyA_site      3677..4155
                     /label="hGH polyA"
     Misc._feature   complement(4195..4335)
                     /label="R-ITR (AAV-2)"
     oriT            4427..4733
                     /label="f1 Origin"
     CDS             5252..6109
                     /cds_type="ORF"
                     /label="AmpicillinR"
     oriT            6260..6927
                     /label="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca CGCGtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagTC
     1441 GActccggaa taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga
     1501 ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg
     1561 aagttatggC GCGCCtcaCA AGTCCTCTTC AGAAATGAGC TTTTGCTCga tccgcctgtt
     1621 gatgtagctg ctcaggtagt ccagcacctc ctggctgatg atgccgttcc aggcggggta
     1681 tctgatgctg ccctcggcgc tgcccttcag ctgctcgatg tgctgccact cctcgatggg
     1741 gttggtctcg tccttcaggg cgatcatctc cttgctgatg gggtcgtagg cgtagtacct
     1801 ggacaccagg gcgaagtagt ggtcggggat ggcggtgatc tggtgggtgt aggtggtcct
     1861 ggccacggcg gaggccctct tgtcgctcca gttgcccacc acgtttgtca gctcggtcag
     1921 gcccttcatg ctcagaaagc tggtcatcag gtgcctgccg atgtggctct tagggccgtt
     1981 cttgatagcg aagatggggt agggggcgtt cttcttcagg gccttgttgt agctgcgcac
     2041 caggttgtcc ttcagcagct ggtactcctg cttgttgctg ctgctgttgc cggtcctgtt
     2101 cactctcttc agcacgggct cgctgttcct caggaactcg tccaggtaca ccagggggtc
     2161 gatcctgcct ctggcgctga aaaagtagat gtgcctggac acgcttgtct tggtctcggt
     2221 caccaggcac tgaatgatca cgcccaggta cttgttctgc accagcttga agctcttggg
     2281 gtccacgttc ttgatgtcgc tgaacctgcc gcagttgatg aatgtggcca ggaacaggaa
     2341 ctggtacagg gtcttggtct tggtgaacct gctggtgtac tcgaagctgt tcaggatctt
     2401 ctcggtgatc tcccagatgc tctcgccctc ggacagcagg gccttcagca tcttcttgct
     2461 gtggctgttg cccttgtcgg cctcctcgct gctctcgaac tgcagctgca ggctggacac
     2521 gatgtcggtg atgtcgctct ggtgcttctg gccgttgtaa gggatgatgg tgaactccca
     2581 ggcggggatc agcttcttca ggctggcctc caggatggtg gccttctggg tcttgtactt
     2641 gaactgcagg ctcttgttca cgatgtcgaa gctcaggctg ttgctgatga tggtgttgta
     2701 gctcatgaag gtggccctct tgatggcggt gccgttgtgg gtgatcatcc agcacaggta
     2761 ggtcagctcg gcggcacagc tggcgatctt ctcgccgctg ggcctctcga atctctccac
     2821 gaactgccgc accagcacct tggggggggt cttgcacagg atgtcgaact ggctcatcac
     2881 cttcctcttc ttcttaggag ccatggtggc GCTAGcataa cttcgtataa agtatcctat
     2941 acgaagttat ttgccttaac ccagaaatta tcactgttat tctttagaat ggtgcaaaga
     3001 ataacttcgt ataatgtatg ctatacgaag ttatgaattc gatatcaagc ttatcgataa
     3061 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3121 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3181 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3241 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3301 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3361 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3421 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3481 ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3541 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3601 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
     3661 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     3721 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     3781 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     3841 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     3901 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     3961 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     4021 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     4081 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     4141 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
     4201 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     4261 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4321 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4381 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     4441 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4501 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4561 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4621 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4681 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4741 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4801 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4861 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4921 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4981 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5041 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5101 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5161 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5221 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5281 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5341 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5401 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5461 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5521 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5581 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5641 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5701 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5761 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5821 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5881 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5941 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6001 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6061 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6121 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6181 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6421 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6901 cctggccttt tgctggcctt ttgctcacat gt
//
