LOCUS       pAAV-CamKIIa-C1V        7318 bp    DNA     linear       13-MAR-2019
FEATURES             Location/Qualifiers
     LTR             1..141
                     /label=L-ITR
     misc_feature    156..1448
                     /label=a-CaMKII(1.3)
     misc_recomb     1489..1980
                     /dnas_title="ChR1"
                     /label=ChR1
     misc_feature    1981..2520
                     /dnas_title="VChR1"
                     /label=VChR1
     misc_feature    1852..1854
                     /label=E122T
     misc_feature    1972..1974
                     /label=E162T
     misc_feature    2530..2724
                     /note="Kv2.1 TS"
     misc_feature    3435..4043
                     /label=WPRE
     polyA_signal    4063..4541
                     /label=hGH polyA
     LTR             4581..4721
                     /label=R-ITR
     rep_origin      4813..5119
                     /label=f1 Origin
     misc_feature    5638..6495
                     /label=AmpR
     rep_origin      6646..7313
                     /label=pUC ori
     CDS             2725..3417
                     /note="mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQT
                     AKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFED
                     GGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKG
                     DIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERS
                     EGRHSTGGMDELYKSGLRSRAQASNSAVDGTAGPGSTGSR"
     source          1..7318
                     /dnas_title="pAAV-CamKIIa-C1V1_(t_t)-KV21TS-mScarlet-WPRE"
ORIGIN      
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTTTAAC ATTATGGCCT TAGGTCACTT
      181 CATCTCCATG GGGTTCTTCT TCTGATTTTC TAGAAAATGA GATGGGGGTG CAGAGAGCTT
      241 CCTCAGTGAC CTGCCCAGGG TCACATCAGA AATGTCAGAG CTAGAACTTG AACTCAGATT
      301 ACTAATCTTA AATTCCATGC CTTGGGGGCA TGCAAGTACG ATATACAGAA GGAGTGAACT
      361 CATTAGGGCA GATGACCAAT GAGTTTAGGA AAGAAGAGTC CAGGGCAGGG TACATCTACA
      421 CCACCCGCCC AGCCCTGGGT GAGTCCAGCC ACGTTCACCT CATTATAGTT GCCTCTCTCC
      481 AGTCCTACCT TGACGGGAAG CACAAGCAGA AACTGGGACA GGAGCCCCAG GAGACCAAAT
      541 CTTCATGGTC CCTCTGGGAG GATGGGTGGG GAGAGCTGTG GCAGAGGCCT CAGGAGGGGC
      601 CCTGCTGCTC AGTGGTGACA GATAGGGGTG AGAAAGCAGA CAGAGTCATT CCGTCAGCAT
      661 TCTGGGTCTG TTTGGTACTT CTTCTCACGC TAAGGTGGCG GTGTGATATG CACAATGGCT
      721 AAAAAGCAGG GAGAGCTGGA AAGAAACAAG GACAGAGACA GAGGCCAAGT CAACCAGACC
      781 AATTCCCAGA GGAAGCAAAG AAACCATTAC AGAGACTACA AGGGGGAAGG GAAGGAGAGA
      841 TGAATTAGCT TCCCCTGTAA ACCTTAGAAC CCAGCTGTTG CCAGGGCAAC GGGGCAATAC
      901 CTGTCTCTTC AGAGGAGATG AAGTTGCCAG GGTAACTACA TCCTGTCTTT CTCAAGGACC
      961 ATCCCAGAAT GTGGCACCCA CTAGCCGTTA CCATAGCAAC TGCCTCTTTG CCCCACTTAA
     1021 TCCCATCCCG TCTGTTAAAA GGGCCCTATA GTTGGAGGTG GGGGAGGTAG GAAGAGCGAT
     1081 GATCACTTGT GGACTAAGTT TGTTCGCATC CCCTTCTCCA ACCCCCTCAG TACATCACCC
     1141 TGGGGGAACA GGGTCCACTT GCTCCTGGGC CCACACAGTC CTGCAGTATT GTGTATATAA
     1201 GGCCAGGGCA AAGAGGAGCA GGTTTTAAAG TGAAAGGCAG GCAGGTGTTG GGGAGGCAGT
     1261 TACCGGGGCA ACGGGAACAG GGCGTTTCGG AGGTGGTTGC CATGGGGACC TGGATGCTGA
     1321 CGAAGGCTCG CGAGGCTGTG AGCAGCCACA GTGCCCTGCT CAGAAGCCCC AAGCTCGTCA
     1381 GTCAAGCCGG TTCTCCGTTT GCACTCAGGA GCACGGGCAG GCGAGTGGCC CCTAGTTCTG
     1441 GGGGCAGCTC TAGAGCGGTA CCGGATCCCC GGGTACCGGT AGGCCACCAT GTCGCGGAGG
     1501 CCATGGCTTC TTGCCCTAGC GCTGGCAGTG GCGCTGGCGG CCGGCAGCGC AGGAGCCTCG
     1561 ACTGGCAGTG ACGCGACGGT GCCGGTCGCG ACTCAGGATG GCCCCGACTA CGTTTTCCAC
     1621 CGTGCCCACG AGCGCATGCT CTTCCAAACC TCATACACTC TTGAGAACAA TGGTTCTGTT
     1681 ATTTGCATCC CGAACAACGG CCAGTGCTTC TGCTTGGCTT GGCTTAAATC CAACGGAACA
     1741 AATGCCGAGA AGTTGGCTGC CAACATTCTG CAGTGGATTA CTTTTGCGCT TTCAGCGCTC
     1801 TGCCTGATGT TCTACGGCTA CCAGACCTGG AAGTCTACTT GCGGCTGGGA GACCATTTAC
     1861 GTGGCCACGA TCGAGATGAT CAAGTTCATC ATCGAGTATT TCCATGAGTT TGACGAACCT
     1921 GCGGTGATCT ACTCATCCAA CGGCAACAAG ACCGTGTGGC TTCGTTACGC GACCTGGCTG
     1981 CTCACCTGCC CGGTCCTTCT GATCCATCTG AGCAACCTCA CAGGCCTGAA GGACGATTAT
     2041 AGCAAAAGGA CTATGGGCCT GTTGGTTTCT GATGTGGGAT GCATCGTGTG GGGCGCAACC
     2101 AGCGCCATGT GTACGGGGTG GACGAAGATC CTGTTCTTCC TCATCTCATT GAGCTATGGT
     2161 ATGTATACCT ATTTTCATGC TGCTAAAGTT TATATCGAAG CATTCCACAC AGTTCCAAAA
     2221 GGGATTTGTC GAGAACTGGT CCGAGTGATG GCCTGGACAT TCTTTGTGGC TTGGGGAATG
     2281 TTTCCAGTCC TGTTTCTGCT GGGCACGGAA GGATTCGGTC ATATCAGCCC TTATGGATCT
     2341 GCCATTGGGC ACTCCATCCT CGACCTGATT GCAAAGAACA TGTGGGGTGT GCTGGGGAAT
     2401 TACCTGCGCG TCAAAATCCA CGAGCACATC CTGTTGTATG GCGACATCAG AAAGAAGCAG
     2461 AAAATTACGA TCGCCGGCCA AGAGATGGAG GTTGAGACAC TGGTGGCTGA AGAGGAGGAC
     2521 GCGGCCGCCc agtcccagcc catcctcaac accaaggaga tggccccgca gagcaagcct
     2581 ccagaggagc tggagatgag cagcatgccc agccccgtgg cccctctgcc cgcacgcacg
     2641 gagggcgtca tcgacatgcg gagcatgtcc agcattgaca gcttcatcag ctgtgccacg
     2701 gacttccctg aagccaccag attcgtgagc aagggcgagg cagtgatcaa ggagttcatg
     2761 cggttcaagg tgcacatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     2821 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     2881 cccctgccct tctcctggga catcctgtcc cctcagttca tgtacggctc cagggccttc
     2941 accaagcacc ccgccgacat ccccgactac tataagcagt ccttccccga gggcttcaag
     3001 tgggagcgcg tgatgaactt cgaggacggc ggcgccgtga ccgtgaccca ggacacctcc
     3061 ctggaggacg gcaccctgat ctacaaggtg aagctccgcg gcaccaactt ccctcctgac
     3121 ggccccgtaa tgcagaagaa gacaatgggc tgggaagcgt ccaccgagcg gttgtacccc
     3181 gaggacggcg tgctgaaggg cgacattaag atggccctgc gcctgaagga cggcggccgc
     3241 tacctggcgg acttcaagac cacctacaag gccaagaagc ccgtgcagat gcccggcgcc
     3301 tacaacgtcg accgcaagtt ggacatcacc tcccacaacg aggactacac cgtggtggaa
     3361 cagtacgaac gctccgaggg ccgccactcc accggcggca tggacgagct gtacaagTAA
     3421 GAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG
     3481 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT
     3541 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG
     3601 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT
     3661 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC
     3721 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC
     3781 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA
     3841 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC
     3901 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG
     3961 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG
     4021 GCCGCCTCCC CGCATCGATA CCGAGCGCTG CTCGAGAGAT CTACGGGTGG CATCCCTGTG
     4081 ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG
     4141 TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG
     4201 GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG
     4261 GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG
     4321 CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT
     4381 GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG
     4441 CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC
     4501 TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTG TAGGTAACCA
     4561 CGTGCGGACC GAGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG
     4621 CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG
     4681 GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CTGCCTGCAG GGGCGCCTGA TGCGGTATTT
     4741 TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG
     4801 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
     4861 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
     4921 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
     4981 TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG
     5041 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC
     5101 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG
     5161 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG
     5221 AATTTTAACA AAATATTAAC GTTTACAATT TTATGGTGCA CTCTCAGTAC AATCTGCTCT
     5281 GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG
     5341 GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG
     5401 TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC
     5461 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT
     5521 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT
     5581 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG
     5641 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT
     5701 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA
     5761 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA
     5821 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT
     5881 ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT
     5941 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC
     6001 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA
     6061 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT
     6121 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT
     6181 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC
     6241 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG
     6301 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC
     6361 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG
     6421 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA
     6481 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA
     6541 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC
     6601 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA
     6661 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
     6721 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA
     6781 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC
     6841 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA
     6901 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA
     6961 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
     7021 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT
     7081 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC
     7141 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC
     7201 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC
     7261 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGT
//

