LOCUS       pAAV-CamKIIa-Chr        7336 bp    DNA     linear       13-MAR-2019
FEATURES             Location/Qualifiers
     LTR             1..141
                     /label=L-ITR
     misc_feature    156..1448
                     /label=a-CaMKII(1.3)
     misc_feature    2548..2742
                     /note="Kv2.1 TS"
     misc_feature    3453..4061
                     /label=WPRE
     polyA_signal    4081..4559
                     /label=hGH polyA
     LTR             4599..4739
                     /label=R-ITR
     rep_origin      4831..5137
                     /label=f1 Origin
     misc_feature    5656..6513
                     /label=AmpR
     rep_origin      6664..7331
                     /label=pUC ori
     CDS             2743..3435
                     /note="mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQT
                     AKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFED
                     GGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKG
                     DIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERS
                     EGRHSTGGMDELYKSGLRSRAQASNSAVDGTAGPGSTGSR"
     misc_feature    1489..2538
                     /note="ChrimsonR"
     source          1..7336
                     /dnas_title="pAAV-CamKIIa-ChrimsonR-KV21TS-mScarlet-WPRE"
ORIGIN      
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTTTAAC ATTATGGCCT TAGGTCACTT
      181 CATCTCCATG GGGTTCTTCT TCTGATTTTC TAGAAAATGA GATGGGGGTG CAGAGAGCTT
      241 CCTCAGTGAC CTGCCCAGGG TCACATCAGA AATGTCAGAG CTAGAACTTG AACTCAGATT
      301 ACTAATCTTA AATTCCATGC CTTGGGGGCA TGCAAGTACG ATATACAGAA GGAGTGAACT
      361 CATTAGGGCA GATGACCAAT GAGTTTAGGA AAGAAGAGTC CAGGGCAGGG TACATCTACA
      421 CCACCCGCCC AGCCCTGGGT GAGTCCAGCC ACGTTCACCT CATTATAGTT GCCTCTCTCC
      481 AGTCCTACCT TGACGGGAAG CACAAGCAGA AACTGGGACA GGAGCCCCAG GAGACCAAAT
      541 CTTCATGGTC CCTCTGGGAG GATGGGTGGG GAGAGCTGTG GCAGAGGCCT CAGGAGGGGC
      601 CCTGCTGCTC AGTGGTGACA GATAGGGGTG AGAAAGCAGA CAGAGTCATT CCGTCAGCAT
      661 TCTGGGTCTG TTTGGTACTT CTTCTCACGC TAAGGTGGCG GTGTGATATG CACAATGGCT
      721 AAAAAGCAGG GAGAGCTGGA AAGAAACAAG GACAGAGACA GAGGCCAAGT CAACCAGACC
      781 AATTCCCAGA GGAAGCAAAG AAACCATTAC AGAGACTACA AGGGGGAAGG GAAGGAGAGA
      841 TGAATTAGCT TCCCCTGTAA ACCTTAGAAC CCAGCTGTTG CCAGGGCAAC GGGGCAATAC
      901 CTGTCTCTTC AGAGGAGATG AAGTTGCCAG GGTAACTACA TCCTGTCTTT CTCAAGGACC
      961 ATCCCAGAAT GTGGCACCCA CTAGCCGTTA CCATAGCAAC TGCCTCTTTG CCCCACTTAA
     1021 TCCCATCCCG TCTGTTAAAA GGGCCCTATA GTTGGAGGTG GGGGAGGTAG GAAGAGCGAT
     1081 GATCACTTGT GGACTAAGTT TGTTCGCATC CCCTTCTCCA ACCCCCTCAG TACATCACCC
     1141 TGGGGGAACA GGGTCCACTT GCTCCTGGGC CCACACAGTC CTGCAGTATT GTGTATATAA
     1201 GGCCAGGGCA AAGAGGAGCA GGTTTTAAAG TGAAAGGCAG GCAGGTGTTG GGGAGGCAGT
     1261 TACCGGGGCA ACGGGAACAG GGCGTTTCGG AGGTGGTTGC CATGGGGACC TGGATGCTGA
     1321 CGAAGGCTCG CGAGGCTGTG AGCAGCCACA GTGCCCTGCT CAGAAGCCCC AAGCTCGTCA
     1381 GTCAAGCCGG TTCTCCGTTT GCACTCAGGA GCACGGGCAG GCGAGTGGCC CCTAGTTCTG
     1441 GGGGCAGCTC TAGAGCGGTA CCGGATCCCC GGGTACCGGT AGGCCACCat ggctgagctg
     1501 atcagcagcg ccaccagatc tctgtttgcc gccggaggca tcaacccttg gcctaacccc
     1561 taccaccacg aggacatggg ctgtggagga atgacaccta caggcgagtg cttcagcacc
     1621 gagtggtggt gtgacccttc ttacggactg agcgacgccg gatacggata ttgcttcgtg
     1681 gaggccacag gcggctacct ggtcgtggga gtggagaaga agcaggcttg gctgcacagc
     1741 agaggcacac caggagaaaa gatcggcgcc caggtctgcc agtggattgc tttcagcatc
     1801 gccatcgccc tgctgacatt ctacggcttc agcgcctgga aggccacttg cggttgggag
     1861 gaggtctacg tctgttgcgt cgaggtgctg ttcgtgaccc tggagatctt caaggagttc
     1921 agcagccccg ccacagtgta cctgtctacc ggcaaccacg cctattgcct gcgctacttc
     1981 gagtggctgc tgtcttgccc cgtgatcctg atcagactga gcaacctgag cggcctgaag
     2041 aacgactaca gcaagcggac catgggcctg atcgtgtctt gcgtgggaat gatcgtgttc
     2101 ggcatggccg caggactggc taccgattgg ctcaagtggc tgctgtatat cgtgtcttgc
     2161 atctacggcg gctacatgta cttccaggcc gccaagtgct acgtggaagc caaccacagc
     2221 gtgcctaaag gccattgccg catggtcgtg aagctgatgg cctacgctta cttcgcctct
     2281 tggggcagct acccaatcct ctgggcagtg ggaccagaag gactgctgaa gctgagccct
     2341 tacgccaaca gcatcggcca cagcatctgc gacatcatcg ccaaggagtt ttggaccttc
     2401 ctggcccacc acctgaggat caagatccac gagcacatcc tgatccacgg cgacatccgg
     2461 aagaccacca agatggagat cggaggcgag gaggtggaag tggaagagtt cgtggaggag
     2521 gaggacgagg acacagtgGC GGCCGCCcag tcccagccca tcctcaacac caaggagatg
     2581 gccccgcaga gcaagcctcc agaggagctg gagatgagca gcatgcccag ccccgtggcc
     2641 cctctgcccg cacgcacgga gggcgtcatc gacatgcgga gcatgtccag cattgacagc
     2701 ttcatcagct gtgccacgga cttccctgaa gccaccagat tcgtgagcaa gggcgaggca
     2761 gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag
     2821 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     2881 aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg
     2941 tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta taagcagtcc
     3001 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc
     3061 gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc
     3121 accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc
     3181 accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat ggccctgcgc
     3241 ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc caagaagccc
     3301 gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc ccacaacgag
     3361 gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac cggcggcatg
     3421 gacgagctgt acaagTAAGA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC
     3481 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA
     3541 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC
     3601 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA
     3661 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC
     3721 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC
     3781 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC
     3841 GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG
     3901 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT
     3961 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG
     4021 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC GAGCGCTGCT CGAGAGATCT
     4081 ACGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC
     4141 CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT
     4201 CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA
     4261 CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT
     4321 GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT
     4381 TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC
     4441 GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC
     4501 CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC
     4561 TGATTTTGTA GGTAACCACG TGCGGACCGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT
     4621 GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG
     4681 ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT GCCTGCAGGG
     4741 GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA
     4801 AAGCAACCAT AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG
     4861 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT
     4921 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA
     4981 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT
     5041 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
     5101 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT
     5161 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT
     5221 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT
     5281 CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC
     5341 GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC
     5401 GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA
     5461 AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG
     5521 ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA
     5581 ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT
     5641 TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG
     5701 GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA
     5761 GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT
     5821 GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT
     5881 GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT
     5941 TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG
     6001 ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA
     6061 CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT
     6121 CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG
     6181 CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA
     6241 CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA
     6301 GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC
     6361 GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT
     6421 ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC
     6481 GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT
     6541 ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT
     6601 TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC
     6661 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC
     6721 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA
     6781 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA
     6841 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT
     6901 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG
     6961 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC
     7021 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA
     7081 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG
     7141 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT
     7201 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG
     7261 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG
     7321 CCTTTTGCTC ACATGT
//

