LOCUS       Exported               10575 bp ds-DNA     circular SYN 25-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Friedel lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 15, 2019 from SnapGene 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        62..90
                     /label=Amp prom
                     /note="color: #4682b4; direction: RIGHT"
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 132 .. 200 / #ccffcc / signal sequence
                        2: 201 .. 992 / #ccffcc
                     Cleavage site after base 200"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             330..989
                     /label=AmpR
                     /note="color: #8fbc8f; direction: RIGHT"
     rep_origin      1141..1769
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 2075 .. 2080 / #ffffff / -35
                        2: 2081 .. 2098 / #ffffff
                        3: 2099 .. 2105 / #ffffff / -10"
     promoter        2075..2104
                     /label=lac
                     /note="color: #4682b4; direction: RIGHT"
     misc_binding    2109..2131
                     /label=LacO
                     /note="color: #6495ed; direction: RIGHT"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     2137..2157
                     /label=M13-rev
                     /note="color: #008b8b; direction: RIGHT"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2175..2194
                     /label=T3
                     /note="color: #008b8b; direction: RIGHT"
     LTR             2448..2628
                     /label=HIV-1 5 LTR
                     /note="color: #ffa500; direction: RIGHT"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    2736..2780
                     /label=HIV-1 psi pack
                     /note="color: #ffa500; direction: RIGHT"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    4019..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        4183..4693
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    4748..4772
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     misc_recomb     4749..4755
                     /label=AttR1
                     /note="color: #dda0dd; direction: RIGHT"
     misc_feature    complement(4756..4772)
                     /label=attL1
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4793..4830
                     /label=38 bases inserted in pENTR
                     /note="color: #99cc00"
     primer_bind     4827..4847
                     /label=akaluc-F1
                     /note="color: black; sequence: CACCATGGTGAGCAAGGGCGA"
     CDS             4831..5547
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 4831 .. 4833 / #e1ff2f
                        2: 4834 .. 4836 / #e1ff2f / 1a
                        3: 4837 .. 5547 / #e1ff2f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     5378..5397
                     /label=akaluc-F2
                     /note="color: black; sequence: ACCAGCAGAACACCCCCATC"
     primer_bind     complement(5430..5448)
                     /label=akaluc-R4
                     /note="color: black; sequence: TGGACTGGTAGCTCAGGTAG"
     misc_feature    5449..5451
                     /label=K to A change in peptide
                     /note="color: #a6acb3"
     CDS             5559..7206
                     /codon_start=3
                     /gene="Akaluc"
                     /product="firefly luciferase mutant protein Akaluc"
                     /label=Akaluc
                     /note="codon-optimized for the mammals and introduced 
                     random mutation"
                     /note="color: #ff0000"
                     /protein_id="BBC77939.1"
                     /translation="MPKTLRRAQRRSTHSKTGPPASSCTKP*SATPWCPAPSPLPTHIF
                     RWTLPTPSTSR*AFGWQKL*GAMG*IQTIGSWCAARIARSSSCPCWVPCSSVWLWPQLT
                     TSTTSASC*TAWASASPPSYS*ARKGCERSSTCKRSYRSYERSSSWIARPTTRASKACT
                     PS*LPICHPASTSTTSCPRASTGTKPSP*S*TVVVVQDYPRA*PYRTAPLVSDSVMPAT
                     PSSATRTSPTPLSSAWCHFTTASACSPRWAT*SAAFGSCSCTASRRSYSCAACKTIRFN
                     LPCWCPHYLAASLRALSSTSTT*AACARSPAAGRRSARR*VRPWPNASAYQASARAMA*
                     QKQPTPS*SPPRGTVSLAQ*ARWCPSSRLRW*TWSPVRHWV*TSAVSCASVAP*S*AAT
                     LTTPRLRTLSSTRTAGCTAATSPTGTRTSTSSSWTG*RA*SNTRATR*PQPNWRASCCN
                     TPTSSTPESPACPTTMPASCPPQSSCWNTVKP*PRKRSWTMWPARLQPPRSCAVVLCLW
                     MKSLEDRPAS*TPARSARFSLRPRRTARSPC"
     primer_bind     5929..5948
                     /label=akaluc-F3
                     /note="color: black; sequence: gtattcgtgagcaagaaagg"
     primer_bind     complement(6029..6048)
                     /label=akaluc-R3
                     /note="color: black; sequence: gtacatgctttggaagccct"
     primer_bind     6452..6471
                     /label=akaluc-F4
                     /note="color: black; sequence: tcatcgacaagtacgaccta"
     primer_bind     complement(6579..6598)
                     /label=akaluc-R2
                     /note="color: black; sequence: cggcgttggttgtttctgtc"
     primer_bind     complement(7186..7206)
                     /label=akaluc-R1
                     /note="color: black; sequence: ttacacggcgatcttgccgtc"
     protein_bind    complement(7223..7247)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    7223..7232
                     /label=attL2
                     /note="color: #a6acb3"
     misc_recomb     complement(7233..7246)
                     /label=AttR2
                     /note="color: #dda0dd; direction: LEFT"
     misc_feature    7278..7866
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7749..7760)
                     /label=FactorXa site
                     /note="color: #008000; direction: LEFT"
     promoter        7907..8246
                     /label=mPGK Prom
                     /note="color: #4682b4; direction: RIGHT"
     promoter        7909..8408
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8422..9225
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8434..9225
                     /label=Kan/neoR
                     /note="color: #8fbc8f; direction: RIGHT"
     LTR             9316..9549
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    9621..9742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      9761..9896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     misc_feature    9761..9838
                     /label=SV40 ORI
                     /note="color: #4682b4; direction: RIGHT"
     primer_bind     complement(9928..9947)
                     /label=T7
                     /note="color: #008b8b; direction: LEFT"
     promoter        complement(9929..9947)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(9957..9974)
                     /label=M13-fwd
                     /note="color: #008b8b; direction: LEFT"
     primer_bind     complement(9957..9973)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     CDS             10045..10113
                     /label=LacZ alpha
                     /note="color: #6495ed; direction: RIGHT"
     rep_origin      10118..10573
                     /direction=RIGHT
                     /label=M13 origin
                     /note="color: #4682b4"
     rep_origin      complement(10123..10563)
                     /direction=LEFT
                     /label=F1 ori
                     /note="color: #4682b4"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc caccggttcg tcgactagtc cagtgtggtg gaattctgca
     4741 gatatcaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccgacatagt
     4801 acagaagggt gggcgcgccc acccttcacc atggtgagca agggcgagga gctgttcacc
     4861 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4921 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
     4981 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag
     5041 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     5101 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     5161 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5221 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     5281 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     5341 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5401 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     5461 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5521 actctcggca tggacgagct gtacaaggga tccatggaag atgccaaaaa cattaagaag
     5581 ggcccagcgc cgttctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     5641 atgaagcgct acgccctggt gcccggcgcc atcgccttta ccgacgcaca tattcaggtg
     5701 gacgttacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaggcgc
     5761 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctc gcagttcttc
     5821 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     5881 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     5941 aagaaagggc tgcgaaaggt cctcaacgtg caaaagaagc taccgatcat acgaaagatc
     6001 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     6061 tcccatttgc cacccagctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     6121 aaaaccatcg ccctgatcat gaacagtagt ggtagtacag gattacccaa gggcgtagcc
     6181 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggctac
     6241 cagaacatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     6301 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     6361 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     6421 acactattta gctgcctcgc taagagcact ctcatcgaca agtacgacct aagcagcttg
     6481 cgcgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     6541 cgcttccgcc taccaggcat ccgccagggc tatggcctga cagaaacaac caacgccgtc
     6601 atgatcaccc ccgaggggga ccgtaagcct ggctcagtag gcaaggtggt gcccttcttc
     6661 gaggctaagg tggtagactt ggtcaccggt aagacactgg gtgtgaacca gcgcggtgag
     6721 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacgaac
     6781 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     6841 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     6901 gccccagccg aactggaggg catcctgctg caacacccct acatcttcga cgccggagtc
     6961 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gttggaacac
     7021 ggtaaaacca tgaccgagaa agagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     7081 aagaagctgc gcggtggtgt tgtgtttgtg gatgaagtcc ctagaggatc gaccggcaag
     7141 ttagacgccc gcaagatccg cgagattctc actaaggcca agaaggacgg caagatcgcc
     7201 gtgtaaaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggttga tatccagcac
     7261 agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7321 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7381 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7441 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7501 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7561 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7621 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     7681 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7741 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7801 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7861 ccccgcctgg aattctgcag atatccggac tagtgatcta attctaccgg gtaggggagg
     7921 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     7981 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     8041 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     8101 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     8161 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     8221 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     8281 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     8341 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     8401 gcctttcgac ctgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt
     8461 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     8521 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     8581 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     8641 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     8701 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     8761 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     8821 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     8881 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     8941 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     9001 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     9061 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     9121 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     9181 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagggga tccgtcgagg
     9241 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9301 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     9361 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctgcctag
     9421 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9481 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9541 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     9601 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     9661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9721 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     9781 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9841 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9901 cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     9961 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    10021 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    10081 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
    10141 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10201 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10261 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10321 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10381 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    10441 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    10501 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    10561 acgtttacaa tttcc
//
