LOCUS       pL_03_G7\(pRS416U-mKate2-P2_TRX2_5xUAS-GFP)      8329 bp    DNA     linear   UNA 
DEFINITION  pRS416.
ACCESSION   pL_04_F2\(pRS416U-mKate2-P2_TDH3_4xUAS-GFP)
VERSION     pL_04_F2\(pRS416U-mKate2-P2_TDH3_4xUAS-GFP).0
KEYWORDS    .
COMMENT     ORIGDB|GenBank
            This file is created by Vector NTI
            http://www.lifetechnologies.com/
            VNTAUTHORNAME|Demo User
            VNTOAUTHORNAME|UNKNOWN
FEATURES             Location/Qualifiers
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
                     /label="pGEX_3_primer"
                     /vntifkey="21"
                     /original_location="complement(29..51)"
     promoter        202..415
                     /label="URA3_promoter"
                     /label="URA3_promoter"
                     /vntifkey="30"
                     /original_location="202..415"
     CDS             417..1220
                     /label="URA3"
                     /label="URA3"
                     /vntifkey="4"
                     /original_location="417..1220"
     misc_feature    complement(1822..1965)
                     /label="lacZ_a"
                     /label="lacZ_a"
                     /vntifkey="21"
                     /original_location="complement(1822..1965)"
     terminator      complement(2024..2218)
                     /label="T ADH1"
                     /label="T ADH1"
                     /vntifkey="43"
                     /original_location="complement(2024..2218)"
     enhancer        2237..2376
                     /label="TRX2_UAS"
                     /label="TRX2_UAS"
                     /vntifkey="9"
     enhancer        2377..2516
                     /label="TRX2_UAS"
                     /label="TRX2_UAS"
                     /vntifkey="9"
     enhancer        2526..2665
                     /label="TRX2_UAS"
                     /label="TRX2_UAS"
                     /vntifkey="9"
     enhancer        2666..2805
                     /label="TRX2_UAS"
                     /label="TRX2_UAS"
                     /vntifkey="9"
     eukaryotic      2815..3089
                     /label="P2_TRX2"
                     /promoter_type="eukaryotic"
                     /vntifkey="29"
     enhancer        3099..3104
                     /label="Kozak"
                     /label="Kozak"
                     /vntifkey="9"
                     /original_location="3130..3135"
     CDS             3105..3821
                     /label="yeGFP"
                     /label="yeGFP"
                     /vntifkey="4"
                     /original_location="3136..3852"
     terminator      3835..4005
                     /label="T CYC1"
                     /label="T CYC1"
                     /vntifkey="43"
                     /original_location="2248..2418"
     terminator      complement(4015..4264)
                     /label="T_IDP1"
                     /label="T_IDP1"
                     /vntifkey="43"
     CDS             complement(4269..4967)
                     /label="mKate2"
                     /label="mKate2"
                     /vntifkey="4"
     eukaryotic      complement(4968..5533)
                     /label="P_TEF2"
                     /label="P_TEF2"
                     /vntifkey="29"
     promoter        complement(5554..5573)
                     /label="T3_promoter"
                     /label="T3_promoter"
                     /vntifkey="30"
                     /original_location="complement(2436..2455)"
     gene            complement(6755..7615)
                     /label="Ampicillin"
                     /label="Ampicillin"
                     /vntifkey="60"
                     /gene="Ampicillin"
                     /original_location="complement(3637..4497)"
     promoter        complement(7657..7685)
                     /label="AmpR_promoter"
                     /label="AmpR_promoter"
                     /vntifkey="30"
                     /original_location="complement(4539..4567)"
     misc_feature    7747..8265
                     /label="CEN6_ARS4"
                     /label="CEN6_ARS4"
                     /vntifkey="21"
                     /original_location="4629..5147"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATACCAC AGCTTTTCAA TTCAATTCAT CATTTTTTTT TTATTCTTTT TTTTGATTTC
      241 GGTTTCTTTG AAATTTTTTT GATTCGGTAA TCTCCGAACA GAAGGAAGAA CGAAGGAAGG
      301 AGCACAGACT TAGATTGGTA TATATACGCA TATGTAGTGT TGAAGAAACA TGAAATTGCC
      361 CAGTATTCTT AACCCAACTG CACAGAACAA AAACCTGCAG GAAACGAAGA TAAATCATGT
      421 CGAAAGCTAC ATATAAGGAA CGTGCTGCTA CTCATCCTAG TCCTGTTGCT GCCAAGCTAT
      481 TTAATATCAT GCACGAAAAG CAAACAAACT TGTGTGCTTC ATTGGATGTT CGTACCACCA
      541 AGGAATTACT GGAGTTAGTT GAAGCATTAG GTCCCAAAAT TTGTTTACTA AAAACACATG
      601 TGGATATCTT GACTGATTTT TCCATGGAGG GCACAGTTAA GCCGCTAAAG GCATTATCCG
      661 CCAAGTACAA TTTTTTACTC TTCGAAGACA GAAAATTTGC TGACATTGGT AATACAGTCA
      721 AATTGCAGTA CTCTGCGGGT GTATACAGAA TAGCAGAATG GGCAGACATT ACGAATGCAC
      781 ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA GAAGTAACAA
      841 AGGAACCTAG AGGCCTTTTG ATGTTAGCAG AATTGTCATG CAAGGGCTCC CTATCTACTG
      901 GAGAATATAC TAAGGGTACT GTTGACATTG CGAAGAGCGA CAAAGATTTT GTTATCGGCT
      961 TTATTGCTCA AAGAGACATG GGTGGAAGAG ATGAAGGTTA CGATTGGTTG ATTATGACAC
     1021 CCGGTGTGGG TTTAGATGAC AAGGGAGACG CATTGGGTCA ACAGTATAGA ACCGTGGATG
     1081 ATGTGGTCTC TACAGGATCT GACATTATTA TTGTTGGAAG AGGACTATTT GCAAAGGGAA
     1141 GGGATGCTAA GGTAGAGGGT GAACGTTACA GAAAAGCAGG CTGGGAAGCA TATTTGAGAA
     1201 GATGCGGCCA GCAAAACTAA AAAACTGTAT TATAAGTAAA TGCATGTATA CTAAACTCAC
     1261 AAATTAGAGC TTCAATTTAA TTATATCAGT TATTACCCTA TGCGGTGTGA AATACCGCAC
     1321 AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT
     1381 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
     1441 TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA
     1501 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG
     1561 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA
     1621 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG
     1681 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA
     1741 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG
     1801 GCGCGTCGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
     1861 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
     1921 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG CGCGCGTAAT
     1981 ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT CGAGAGCGAC CTCATGCTAT
     2041 ACCTGAGAAA GCAACCTGAC CTACAGGAAA GAGTTACTCA AGAATAAGAA TTTTCGTTTT
     2101 AAAACCTAAG AGTCACTTTA AAATTTGTAT ACACTTATTT TTTTTATAAC TTATTTAATA
     2161 ATAAAAATCA TAAATCATAA GAAATTCGCT TATTTAGAAG TGTCAACAAC GTATCTACCA
     2221 ACGGAATGCG TGCGATCTTG AGTGCCAGTG AATATTAAGT AATCATCAAA GTATATGTGT
     2281 AATTGTTTAT ACTCTTAGTA AAGGATGCTC CCTACAAGGT GGCTCTTTTC TTACTAAGCG
     2341 CGTTCAGTTT CCAGCCAGCC GAAAGAGGGA TATCAGCTTG AGTGCCAGTG AATATTAAGT
     2401 AATCATCAAA GTATATGTGT AATTGTTTAT ACTCTTAGTA AAGGATGCTC CCTACAAGGT
     2461 GGCTCTTTTC TTACTAAGCG CGTTCAGTTT CCAGCCAGCC GAAAGAGGGA TATCAGACCT
     2521 GCACTCTTGA GTGCCAGTGA ATATTAAGTA ATCATCAAAG TATATGTGTA ATTGTTTATA
     2581 CTCTTAGTAA AGGATGCTCC CTACAAGGTG GCTCTTTTCT TACTAAGCGC GTTCAGTTTC
     2641 CAGCCAGCCG AAAGAGGGAT ATCAGCTTGA GTGCCAGTGA ATATTAAGTA ATCATCAAAG
     2701 TATATGTGTA ATTGTTTATA CTCTTAGTAA AGGATGCTCC CTACAAGGTG GCTCTTTTCT
     2761 TACTAAGCGC GTTCAGTTTC CAGCCAGCCG AAAGAGGGAT ATCAGATCTG TCATCTTGAG
     2821 TGCCAGTGAA TATTAAGTAA TCATCAAAGT ATATGTGTAA TTGTTTATAC TCTTAGTAAA
     2881 GGATGCTCCC TACAAGGTGG CTCTTTTCTT ACTAAGCGCG TTCAGTTTCC AGCCAGCCGA
     2941 AAGAGGGATA TCAGTATATA AGAAAGCCAT TCGGGGGATG AAAAGCTGAC AAGAGAATAA
     3001 CGAGGACCAG TTTTTATTTG TTGTCTAGCA AGAATTATAC ACGCACACAT ACACGAGAGT
     3061 CTACGATATC TTTAAATAAC ACATCAATAA TCTGTCATAA AACAATGTCT AAAGGTGAAG
     3121 AATTATTCAC TGGTGTTGTC CCAATTTTGG TTGAATTAGA TGGTGATGTT AATGGTCACA
     3181 AATTTTCTGT CTCCGGTGAA GGTGAAGGTG ATGCTACTTA CGGTAAATTG ACCTTAAAAT
     3241 TTATTTGTAC TACTGGTAAA TTGCCAGTTC CATGGCCAAC CTTAGTCACT ACTTTCGGTT
     3301 ATGGTGTTCA ATGTTTTGCG AGATACCCAG ATCATATGAA ACAACATGAC TTTTTCAAGT
     3361 CTGCCATGCC AGAAGGTTAT GTTCAAGAAA GAACTATTTT TTTCAAAGAT GACGGTAACT
     3421 ACAAGACCAG AGCTGAAGTC AAGTTTGAAG GTGATACCTT AGTTAATAGA ATCGAATTAA
     3481 AAGGTATTGA TTTTAAAGAA GATGGTAACA TTTTAGGTCA CAAATTGGAA TACAACTATA
     3541 ACTCTCACAA TGTTTACATC ATGGCTGACA AACAAAAGAA TGGTATCAAA GTTAACTTCA
     3601 AAATTAGACA CAACATTGAA GATGGTTCTG TTCAATTAGC TGACCATTAT CAACAAAATA
     3661 CTCCAATTGG TGATGGTCCA GTCTTGTTAC CAGACAACCA TTACTTATCC ACTCAATCTG
     3721 CCTTATCCAA AGATCCAAAC GAAAAGAGAG ACCACATGGT CTTGTTAGAA TTTGTTACTG
     3781 CTGCTGGTAT TACCCATGGT ATGGATGAAT TGTACAAATA AATCGCGTGC ATTCATCCGC
     3841 TCTAACCGAA AAGGAAGGAG TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT
     3901 AGTTATGTTA GTATTAAGAA CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG
     3961 ACGCGTGTAC GCATGTAACA TTATACTGAA AACCTTGCTT GAGAAGAGCT CGAGAACAGC
     4021 CCCCTAAAAC ACGGTAATGA AGAAGAAGAA CTCATGAAAA TGTTGGAAAT TGATGGTAAT
     4081 GATCCGAACT TGGGGAACCT TGATAAGGAA TTAACCGACG GTTGCAAACA ACAATAAGGT
     4141 ATATATATTT ATGATAACAG TAAAAAAAAA AAAAAAAAAG TAGTTCATTA TCCTAGAGCA
     4201 TAACTAAGTA TAACACTTTA AAAAATGAAA AAAAAAAGTG GTAGATTGGG CTACGTAAAT
     4261 TCGAAGCTTT ATCTGTGTCC CAACTTAGAT GGCAAATCAC AGTATCTAGC CACTGCCACT
     4321 TCGTGTTGTT CAACATAAGT CTCTTTATCA GCCTCTTTAA TCCTTTCTAA TCTTCGGTCG
     4381 ACGTAGTAAA CACCTGGCAT CTTTAGGTTT TTGGCTGGCT TTTTAGAACG ATAAGTGGTT
     4441 TTCAAATTGC AGATTAGATG CCCTCCACCA ACCAGTTTAA GGGCCATGTC AGCTCTGCCT
     4501 TCTAGGCCAC CATCAGCAGG GTACAGGGTT TCGGTTGACG CTTCCCAGCC TAATGTCTTT
     4561 TTCTGCATAA CTGGACCATT AGAAGGGAAA TTGACGCCTC TAATCTTGAC ATTGTAGATT
     4621 AAACACCCAT CTTGAAGTGA TGTGTCTTGA GTGGCTGTCA ATACACCTCC ATCCTCGTAC
     4681 GTTGTTACTC TTTCCCATGT AAAACCCTCT GGAAAACTCT GTTTGAAAAA GTCTGGTATA
     4741 CCTTGAGTAT GGTTTATGAA TGTCTTGCTC CCATACATGA ATGATGTTGC CAAGATATCA
     4801 AATGCAAAAG GTAATGGTCC ACCTTCTACA GCTTTAATTC TCATAGTTTG AGTTCCTTCG
     4861 TAAGGTTTGC CTTCACCCTC GGATGTACAC TTAAAATGAT GATTGTTCAC AGTACCTTCC
     4921 ATGTAAAGTT TCATGTGCAT GTTTTCCTTG ATGAGTTCAG AAACCATGTT TAGTTAATTA
     4981 TAGTTCGTTG ACCGTATATT CTAAAAACAA GTACTCCTTA AAAAAAAACC TTGAAGGGAA
     5041 TAAACAAGTA GAATAGATAG AGAGAAAAAT AGAAAATGCA AGAGAATTTA TATATTAGAA
     5101 AGAGAGAAAG AAAAATGGAA AAAAAAAAAT AGGAAAAGCC AGAAATAGCA CTAGAAGGAG
     5161 CGACACCAGA AAAGAAGGTG ATGGAACCAA TTTAGCTATA TATAGTTAAC TACCGGCTCG
     5221 ATCATCTCTG CCTCCAGCAT AGTCGAAGAA GAATTTTTTT TTTCTTGAGG CTTCTGTCAG
     5281 CAACTCGTAT TTTTTCTTTC TTTTTTGGTG AGCCTAAAAA GTTCCCACGT TCTCTTGTAC
     5341 GACGCCGTCA CAAACAACCT TATGGGTAAT TTGTCGCGGT CTGGGTGTAT AAATGTGTGG
     5401 GTGCAACATG AATGTACGGA GGTAGTTTGC TGATTGGCGG TCTATAGATA CCTTGGTTAT
     5461 GGCGCCCTCA CAGCCGGCAG GGGAAGCGCC TACGCTTGAC ATCTACTATA TGTAAGTATA
     5521 CGGCCCCATA TATCTCGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT
     5581 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
     5641 ACAACATAGG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGGTAAC
     5701 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC
     5761 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
     5821 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     5881 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     5941 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     6001 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     6061 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     6121 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     6181 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     6241 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     6301 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     6361 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     6421 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     6481 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     6541 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     6601 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     6661 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     6721 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     6781 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     6841 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
     6901 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     6961 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     7021 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     7081 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     7141 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     7201 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     7261 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     7321 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     7381 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     7441 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     7501 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     7561 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     7621 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     7681 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     7741 CACCTGGGTC CTTTTCATCA CGTGCTATAA AAATAATTAT AATTTAAATT TTTTAATATA
     7801 AATATATAAA TTAAAAATAG AAAGTAAAAA AAGAAATTAA AGAAAAAATA GTTTTTGTTT
     7861 TCCGAAGATG TAAAAGACTC TAGGGGGATC GCCAACAAAT ACTACCTTTT ATCTTGCTCT
     7921 TCCTGCTCTC AGGTATTAAT GCCGAATTGT TTCATCTTGT CTGTGTAGAA GACCACACAC
     7981 GAAAATCCTG TGATTTTACA TTTTACTTAT CGTTAATCGA ATGTATATCT ATTTAATCTG
     8041 CTTTTCTTGT CTAATAAATA TATATGTAAA GTACGCTTTT TGTTGAAATT TTTTAAACCT
     8101 TTGTTTATTT TTTTTTCTTC ATTCCGTAAC TCTTCTACCT TCTTTATTTA CTTTCTAAAA
     8161 TCCAAATACA AAACATAAAA ATAAATAAAC ACAGAGTAAA TTCCCAAATT ATTCCATCAT
     8221 TAAAAGATAC GAGGCGCGTG TAAGTTACAG GCAAGCGATC CGTCCTAAGA AACCATTATT
     8281 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTC
//
