LOCUS       Exported                7857 bp ds-DNA     circular DNA 18-MAR-2019
DEFINITION  pcDNA3.1/Puro-CAG-ASAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 18, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="pcDNA3.1/Puro-CAG-ASAP1"
                     /mol_type="other DNA"
     primer_bind     1..29
                     /label=pcDNA3.1-mac-f
     gene            14..967
                     /label=Mac
     primer_bind     458..476
                     /label=Macseqf
     primer_bind     complement(950..997)
                     /label=mac-BFP2-r
     gene            968..1681
                     /label=bfp2
     unsure          1665..1795
                     /label=pcDNA3.1-ArchT-r
     primer_bind     complement(1667..1724)
                     /label=TS+ERex-rv1
     gene            1682..1771
                     /label=TS+ERex
     primer_bind     complement(1710..1764)
                     /label=TS+ERex-rv2
     primer_bind     complement(1748..1795)
                     /label=TS+ERex-rv3
     primer_bind     1772..1785
                     /label=pcDNA3.1-linear-f
     misc_feature    complement(1880..1897)
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     terminator      1883..2110
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     rep_origin      2173..2479
                     /label=f1_origin
                     /label=f1_origin
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     misc_feature    complement(2593..2613)
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     misc_feature    complement(2599..2814)
                     /label=SV40_enhancer
                     /label=SV40_enhancer
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     promoter        2611..2879
                     /label=SV40_promoter
                     /label=SV40_promoter
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     rep_origin      2778..2855
                     /label=SV40_origin
                     /label=SV40_origin
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     misc_feature    2840..2859
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     gene            2936..3535
                     /gene="puro (variant)"
                     /label=puro (variant)
                     /label=puro (variant)
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     CDS             2936..3535
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     terminator      3698..3817
                     /label=SV40_PA_terminator
                     /label=SV40_PA_terminator
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3786..3805
                     /label=EBV_rev_primer
                     /label=EBV_rev_primer
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     primer_bind     complement(3843..3862)
                     /label=SV40polyA-2
     promoter        complement(3861..3879)
                     /label=M13_reverse_primer
                     /label=M13_reverse_primer
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    complement(3878..3900)
                     /label=M13_pUC_rev_primer
                     /label=M13_pUC_rev_primer
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     promoter        complement(3914..3943)
                     /label=lac_promoter
                     /label=lac_promoter
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     primer_bind     4326..4343
                     /label=f-xa.7
     primer_bind     complement(4328..4345)
                     /label=f-xa.J
     primer_bind     4772..4789
                     /label=f-xa.9
     gene            complement(5023..5883)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     CDS             complement(5023..5883)
                     /codon_start=1
                     /label=ORF frame 1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5234..5251
                     /label=f-xa.11
     primer_bind     complement(5240..5257)
                     /label=f-xa.E
     promoter        complement(5925..5953)
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    6114..6401
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     CDS             complement(6770..7378)
                     /codon_start=1
                     /label=ORF frame 3
                     /label=ORF frame 3
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPARH
                     GEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     APRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPAQRA
                     LPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE"
     misc_feature    7713..7732
                     /label=pCAG_F_primer
                     /label=pCAG_F_primer
                     /note="/translation=MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLP
                     PPPAR 
                     HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA 
                     QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
     primer_bind     7833..7857
                     /label=pcDNA3.1-CarO-f
     primer_bind     complement(7851..7857)
                     /label=pcDNA3.1-linear-r
                     /label=nonstandard type: primer
ORIGIN
        1 gctagccgcc accatgatcg tggaccagtt cgaggaggtg ctgatgaaga ccagccagct
       61 gttcccactg ccaaccgcta cccagagcgc ccagccaacc cacgtggccc ccgtgccaac
      121 cgtgctgccc gacaccccca tctacgagac cgtgggcgac agcggcagca agaccctgtg
      181 ggtggtgttc gtgctgatgc tgatcgccag cgccgccttc accgccctga gctggaagat
      241 ccccgtgaac aggaggctgt accacgtgat caccaccatc atcaccctga ccgccgccct
      301 gagctacttc gctatggcta ccggccacgg agtggccctg aacaagatcg tgatcaggac
      361 ccagcacgac cacgtgcccg acacctacga gaccgtgtac cgacaggtgt actacgccag
      421 gtacatcgac tgggctatca ccaccccact gctgctgctg gacctgggac tgctggctgg
      481 aatgagcgga gcccacatct tcatggccat cgtggctgac ctgatcatgg tgctgaccgg
      541 cctgttcgct gctttcggca gcgagggaac cccacagaag tggggatggt acaccatcgc
      601 ctgcatcgcc tacatcttcg tggtgtggca cctggtgctg aacggcggcg ccaacgccag
      661 ggtgaagggc gagaagctga ggagcttctt cgtggccatc ggagcttaca ccctgatcct
      721 gtggaccgct tacccaatcg tgtggggact ggctgacgga gctaggaaga tcggagtgga
      781 cggagagatc atcgcttacg ctgtgctgga cgtgctggct aagggagtgt tcggagcttg
      841 gctgctggtg acccacgcca acctgaggga gagcgacgtg gagctgaacg gcttctgggc
      901 caacggcctg aacagggagg gcgccatcag gatcggcgag gacgacggcg cccgaccggt
      961 agtagcagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1021 ggacggcgac gtaaacggcc acaagttcag cgtgaggggc gagggcgagg gcgatgccac
     1081 caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1141 caccctcgtg accaccctga gccacggcgt gcagtgcttc gcccgctacc ccgaccacat
     1201 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1261 cttcttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac
     1321 cctggtgaac cgcatcgagc tgaagggcgt cgacttcaag gaggacggca acatcctggg
     1381 gcacaagctg gagtacaact tcaacagcca caacatctat atcatggccg tcaagcagaa
     1441 gaacggcatc aaggtgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct
     1501 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacag
     1561 ccactacctg agcacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat
     1621 ggtcctgctg gagttccgca ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1681 gaagcttaaa agtagaatca caagcgaagg cgagtacatc cccctggatc aaatagacat
     1741 aaatgtaggt ggattttgtt atgagaatga atgaggtacc gagctcggat ccactagtcc
     1801 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
     1861 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     1921 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     1981 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2041 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2101 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     2161 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2221 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2281 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2341 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     2401 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2461 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2521 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2581 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2641 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     2701 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     2761 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     2821 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     2881 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccatgac
     2941 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     3001 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     3061 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3121 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3181 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     3241 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     3301 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     3361 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     3421 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     3481 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgattcga
     3541 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     3601 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     3661 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     3721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     3841 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3901 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3961 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4021 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4081 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4141 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4201 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4261 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4321 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4381 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4441 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4501 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4561 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4621 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4681 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4741 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4801 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5641 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5881 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5941 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6001 aaaagtgcca cctgacgtcg acggatcggg gtcgacattg attattgact agttattaat
     6061 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     6241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     6301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     6361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     6421 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     6481 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggcgcgcgcc
     6541 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     6601 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     6661 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     6721 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     6781 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     6841 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     6901 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     6961 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     7021 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     7081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     7141 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     7201 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     7261 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     7321 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     7381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     7441 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     7501 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     7561 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     7621 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     7681 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     7741 tcattttggc aaagaattcc tcgaggaatt cactcctcag gtgcaggctg cctatcagaa
     7801 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tgcatga
//
