LOCUS       Exported                5779 bp ds-DNA     circular SYN 18-MAR-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pHybr_r2a mG1-srt-his
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, March 18, 2019 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     293..309
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        320..338
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    370..984
                     /note="5' Homology arm"
                     /note="color: #ffffff"
     misc_feature    992..1026
                     /note="Splice"
                     /note="color: #a6acb3"
     CDS             1029..2003
                     /codon_start=1
                     /note="mIgG1"
                     /note="color: #993366"
                     /translation="KTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSL
                     SSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCK
                     PCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTA
                     QTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAP
                     QVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYF
                     VYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGKRT"
     CDS             2004..2018
                     /codon_start=1
                     /note="G4S"
                     /note="color: #ffff00"
                     /translation="GGGGS"
     CDS             2019..2036
                     /codon_start=1
                     /note="LPETGG sortag"
                     /note="color: #339966"
                     /translation="LPETGG"
     CDS             2037..2054
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     misc_feature    2071..2644
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     CDS             2667..3065
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="BSD"
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3078..3307
                     /note="SV40 polyA"
                     /note="color: #a6acb3"
     misc_feature    3308..3870
                     /note="3' Homology arm"
                     /note="color: #ffffff"
     rep_origin      complement(4095..4683)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(4857..5651)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5652..5756)
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcata gaaagatctg agtagaacca aggtaaaaag tgtgggtaaa aacacatgtt
      421 cacaggcctg gctgacatga tgctgggcac gtatggaggc aaagtcaaga gggcagtgta
      481 agggccagaa gtgaatcctg acccaagaat agagagtgct aaacctacgt agatcgaagc
      541 caactaaaaa gacaagctac aaaacgaagc taaggccaga gatcttggac tgtgaagagt
      601 tcagagaacc taggatcagg aaccattagt aacaggccaa ggaagataga agctgcctag
      661 gacttggcaa gagccaacat ggttggactg gaaaagaaag gaggagacag aagacaggag
      721 agatgtgcca acttgatttt gggcttcact gttgtccata ctgtgtgcag ccatatggcc
      781 cacagataac aggtttagcc gaggaacaca gatacccaca ttggacaatg gtgggggaac
      841 acagataccc atactacagg gctctttagg gcatttcctg aaagtgtact aggagtggga
      901 ctgggctcaa agggattagg tgtgatctgg cctggtgagg ctgacattgg caagcccaat
      961 ggttgggtgt tgcctcctcc atgtgctagc gatcgcaggc gcaatcttcg catttctttt
     1021 ttccagcaaa gaccacacca ccttctgtgt acccactcgc accaggcagc gccgctcaaa
     1081 ccaacagtat ggtgaccttg gggtgtcttg tgaagggcta ctttcccgag cccgttaccg
     1141 tcacctggaa ctccgggtct ctctcaagcg gcgttcacac cttccccgcc gtactgcaat
     1201 cagacctcta taccctgtct tccagcgtca ctgtacccag ttccacctgg cccagtgaaa
     1261 ctgtcacatg taatgtcgca cacccagctt cctccaccaa agtggacaaa aagatcgtgc
     1321 ctagggactg tggatgcaag ccatgtatct gcactgtccc cgaggtttca agtgtattta
     1381 tcttcccccc aaagcccaaa gatgtcctca ccataacact cacacccaag gtgacctgcg
     1441 tggtggtcga tatcagtaaa gatgaccccg aggtccaatt cagctggttt gtggatgatg
     1501 tagaggtcca cactgctcag actcagccac gggaagagca gtttaactca acatttcgga
     1561 gtgtgtccga actgccaatc atgcaccaag attggctcaa tgggaaagaa ttcaaatgca
     1621 gggtgaatag tgccgccttt cctgcaccaa tagagaaaac catatcaaag acaaagggca
     1681 ggcccaaggc ccctcaagtc tatactatac ctccacccaa ggagcaaatg gctaaagata
     1741 aggtaagcct cacctgcatg atcacagatt ttttcccaga agacataact gtcgaatggc
     1801 aatggaacgg ccaacctgca gaaaattaca agaatactca gcctattatg gacaccgatg
     1861 gcagctactt cgtgtattca aaactgaacg tacagaaatc taattgggaa gcagggaata
     1921 cattcacatg ctctgtgttg catgaagggc tccataacca ccacactgaa aagagcttga
     1981 gccactctcc cggaaagcgt acgggaggcg gaggcagcct gccggaaacc ggcggccatc
     2041 atcatcatca tcattgagtc gacgtcgagg cccctctccc tccccccccc ctaacgttac
     2101 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     2161 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     2221 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     2281 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     2341 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     2401 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     2461 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     2521 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     2581 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     2641 ataatatggc cacagaattc gccaccatgg ccaagccttt gtctcaagaa gaatccaccc
     2701 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     2761 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     2821 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     2881 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     2941 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     3001 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     3061 gctaagtact agtcgagtgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3121 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3181 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3241 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3301 gagatcttgt acaacttggg gagggtacaa aatggaggac ttgtaggagc ttgggtccag
     3361 acctgtcaga caaaatgatc acgcatactt attcttgttg aaacaacagc cccatctgtc
     3421 tatccactgg ctcctggaac tgctctcaaa agtaactcca tggtgaccct gggatgcctg
     3481 gtcaagggct atttccctga gccagtcacc gtgacctgga actctggagc cctgtccagc
     3541 ggtgtgcaca ccttcccagc tgtcctgcag tctggactct acactctcac cagctcagtg
     3601 actgtaccct ccagcacctg gtccagccag gccgtcacct gcaacgtagc ccacccggcc
     3661 agcagcacca aggtggacaa gaaaattggt gagagaacaa ccaggggatg aggggctcac
     3721 tagaggtgag gataaggcat tagattgcct acaccaacca gggtgggcag acatcaccag
     3781 ggagggggcc tcagcccagg agaccaaaaa ttctcctttg tctcccttct ggagatttct
     3841 atgtccttta cacccattta ttaatattct ctgggcctca tgggccttcc tttcactgcc
     3901 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa catggtcata gctgtttcct
     3961 tgcgtattgg gcgctctccg cttcctcgct cactgactcg ctgcgctcgg tcgttcgggt
     4021 aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4081 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4141 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4201 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4261 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4321 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4381 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4441 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4501 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4561 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4621 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4681 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4741 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4801 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttattag
     4861 aaaaattcat ccagcagacg ataaaacgca atacgctggc tatccggtgc cgcaatgcca
     4921 tacagcacca gaaaacgatc cgcccattcg ccgcccagtt cttccgcaat atcacgggtg
     4981 gccagcgcaa tatcctgata acgatccgcc acgcccagac ggccgcaatc aataaagccg
     5041 ctaaaacggc cattttccac cataatgttc ggcaggcacg catcaccatg ggtcaccacc
     5101 agatcttcgc catccggcat gctcgctttc agacgcgcaa acagctctgc cggtgccagg
     5161 ccctgatgtt cttcatccag atcatcctga tccaccaggc ccgcttccat acgggtacgc
     5221 gcacgttcaa tacgatgttt cgcctgatga tcaaacggac aggtcgccgg gtccagggta
     5281 tgcagacgac gcatggcatc cgccataatg ctcacttttt ctgccggcgc cagatggcta
     5341 gacagcagat cctgacccgg cacttcgccc agcagcagcc aatcacggcc cgcttcggtc
     5401 accacatcca gcaccgccgc acacggaaca ccggtggtgg ccagccagct cagacgcgcc
     5461 gcttcatcct gcagctcgtt cagcgcaccg ctcagatcgg ttttcacaaa cagcaccgga
     5521 cgaccctgcg cgctcagacg aaacaccgcc gcatcagagc agccaatggt ctgctgcgcc
     5581 caatcatagc caaacagacg ttccacccac gctgccgggc tacccgcatg caggccatcc
     5641 tgttcaatca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5761 tttccccgaa aagtgccac
//
