LOCUS       pCAD104_Asn152Ser_pCMV6Entry-hIRG1        6274 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  .
ACCESSION   urn.local...2a-a8tu8ye
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   DG
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 12, 2016 from SnapGene Viewer 3.1.2 to Vector
            NTI(R) format http://www.snapgene.com
FEATURES             Location/Qualifiers
     primer_bind     complement(107..127)
                     /created_by="primer3"
                     /%GC="57.1"
                     /Tm="63.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AATCGCCTTGCAGCACATCCC"
                     /Transferred_From="pTARp75-F1"
                     /Transferred_Similarity="100.00%"
                     /label="pTARp75-F1"
     primer_bind     123..143
                     /created_by="primer3"
                     /%GC="47.6"
                     /Tm="58.7"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGATTAAGTTGGGTAACGCCA"
                     /Transferred_From="pmal_3"
                     /Transferred_Similarity="100.00%"
                     /label="pmal_3"
     primer_bind     159..182
                     /created_by="primer3"
                     /%GC="54.2"
                     /Tm="66.0"
                     /Hairpin_Tm="56.5"
                     /Self_Dimer_Tm="27.6"
                     /Sequence="CGACGTTGTAAAACGACGGCCAGT"
                     /Transferred_From="M13UP"
                     /Transferred_Similarity="100.00%"
                     /label="M13UP"
     primer_bind     160..181
                     /created_by="primer3"
                     /%GC="54.5"
                     /Tm="62.5"
                     /Hairpin_Tm="53.9"
                     /Self_Dimer_Tm="14.1"
                     /Sequence="GACGTTGTAAAACGACGGCCAG"
                     /Transferred_From="M13-FORWARD"
                     /Transferred_Similarity="100.00%"
                     /label="M13-FORWARD"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     primer_bind     419..444
                     /created_by="primer3"
                     /%GC="42.3"
                     /Tm="60.5"
                     /Hairpin_Tm="34.1"
                     /Self_Dimer_Tm="6.5"
                     /Sequence="CGTTACATAACTTACGGTAAATGGCC"
                     /Transferred_From="pTARp75-F2"
                     /Transferred_Similarity="100.00%"
                     /label="pTARp75-F2"
     primer_bind     complement(451..469)
                     /created_by="primer3"
                     /%GC="73.7"
                     /Tm="66.1"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGTCGTTGGGCGGTCAGC"
                     /Transferred_From="pOPIN-R1"
                     /Transferred_Similarity="100.00%"
                     /label="pOPIN-R1"
     misc_feature    591..596
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     877..897
                     /created_by="primer3"
                     /%GC="66.7"
                     /Tm="67.0"
                     /Hairpin_Tm="52.4"
                     /Self_Dimer_Tm="3.5"
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Transferred_From="CMVfor"
                     /Transferred_Similarity="100.00%"
                     /label="CMVfor"
     misc_feature    922..927
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, SstI"
                     /note="Geneious type: restriction site"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     misc_feature    979..984
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     ORF             985..2517
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /label="ORF 2 (frame 1)"
     gene            985..>2427
                     /gene="ACOD1"
                     /gene_synonym="CAD; IRG1"
                     /note="aconitate decarboxylase 1"
                     /db_xref="GeneID:730249"
                     /db_xref="HGNC:HGNC:33904"
                     /db_xref="MIM:615275"
     source          985..>2427
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome=13
                     /map="13q22.3"
     exon            985..996
                     /gene="ACOD1"
                     /gene_synonym="CAD; IRG1"
                     /inference="alignment:Splign:1.39.8"
     exon            997..1158
                     /gene="ACOD1"
                     /gene_synonym="CAD; IRG1"
                     /inference="alignment:Splign:1.39.8"
     exon            1159..1248
                     /gene="ACOD1"
                     /gene_synonym="CAD; IRG1"
                     /inference="alignment:Splign:1.39.8"
     exon            1249..1454
                     /gene="ACOD1"
                     /gene_synonym="CAD; IRG1"
                     /inference="alignment:Splign:1.39.8"
     primer_bind     1358..1377
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.4"
                     /Sequence="TTTCTGGCCTTGACCTGCTG"
                     /Transferred_From="CAD29s"
                     /Transferred_Similarity="100.00%"
                     /label="CAD29s"
     misc_feature    1439
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     exon            1455..>2427
                     /gene="ACOD1"
                     /gene_synonym="CAD; IRG1"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2430..2437
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    2437..2442
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     CDS             2440..2469
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label="Myc"
     misc_feature    2479..2484
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /label="EcoRV"
                     /note="Geneious type: restriction site"
     CDS             2488..2511
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                      cleavage site"
                     /label="FLAG"
     primer_bind     complement(2530..2547)
                     /created_by="primer3"
                     /%GC="50.0"
                     /Tm="52.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGGAAACAGCTATGACC"
                     /Transferred_From="M13rev(-29)"
                     /Transferred_Similarity="100.00%"
                     /label="M13rev(-29)"
     misc_feature    2555..2560
                     /Recognition_pattern="C^CCGGG"
                     /annotation_group="CCCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    2555..2560
                     /Recognition_pattern="CCC^GGG"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     polyA_signal    2558..3180
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     primer_bind     complement(2618..2638)
                     /Sequence="TAGGACAAGGCTGGTGGGCAC"
                     /%GC="61.9"
                     /Transferred_From="C-CMV-24"
                     /Transferred_Similarity="100.00%"
                     /label="C-CMV-24"
     primer_bind     complement(2622..2640)
                     /Transferred_From="IntXL39"
                     /Transferred_Similarity="100.00%"
                     /Sequence="ATTAGGACAAGGCTGGTGG"
                     /Hairpin_Tm="None"
                     /Tm="56.7"
                     /Self_Dimer_Tm="None"
                     /%GC="52.6"
                     /modified_by="kbu07"
                     /label="IntXL39"
     primer_bind     complement(2622..2638)
                     /Transferred_From="XL39-rev"
                     /Transferred_Similarity="100.00%"
                     /Sequence="TAGGACAAGGCTGGTGG"
                     /%GC="58.8"
                     /modified_by="kbu07"
                     /label="XL39-rev"
     primer_bind     3299..3320
                     /created_by="primer3"
                     /%GC="54.5"
                     /Tm="63.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.3"
                     /Sequence="GAACCGTAAAAAGGCCGCGTTG"
                     /Transferred_From="pOPIN-F3"
                     /Transferred_Similarity="100.00%"
                     /label="pOPIN-F3"
     primer_bind     complement(3315..3334)
                     /Sequence="TGGAAAAACGCCAGCAACGC"
                     /Hairpin_Tm="None"
                     /Tm="62.9"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="16.7"
                     /Product_Size=2242
                     /modified_by="kbu07"
                     /label="CMV3315R"
     rep_origin      complement(3329..3917)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     misc_feature    3582..3587
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(3612..3636)
                     /created_by="primer3"
                     /%GC="52.0"
                     /Tm="64.5"
                     /Hairpin_Tm="58.7"
                     /Self_Dimer_Tm="10.8"
                     /Sequence="GACGATAGTTACCGGATAAGGCGCA"
                     /Transferred_From="pOPIN-R5"
                     /Transferred_Similarity="100.00%"
                     /label="pOPIN-R5"
     primer_bind     3895..3918
                     /created_by="primer3"
                     /%GC="45.8"
                     /Tm="61.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGCGCAGAAAAAAAGGATCTCAAG"
                     /Transferred_From="pOPIN-F4"
                     /Transferred_Similarity="100.00%"
                     /label="pOPIN-F4"
     polyA_signal    4246..4293
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4525..5319)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418
                      (Geneticin(R))"
                     /label="NeoR/KanR"
     primer_bind     5140..5163
                     /created_by="primer3"
                     /%GC="58.3"
                     /Tm="65.7"
                     /Hairpin_Tm="64.8"
                     /Self_Dimer_Tm="4.7"
                     /Sequence="CAGTTCATTCAGGGCACCGGACAG"
                     /Transferred_From="dneo1-AS"
                     /Transferred_Similarity="100.00%"
                     /label="dneo1-AS"
     misc_feature    5347..5352
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI"
                     /note="Geneious type: restriction site"
     promoter        complement(5354..5711)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     misc_feature    5367..5372
                     /Recognition_pattern="AGG^CCT"
                     /annotation_group="AGGCCT"
                     /label="StuI"
                     /note="Geneious type: restriction site"
     rep_origin      5368..5503
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     misc_feature    5411..5423
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /annotation_group="GGCCNNNNNGGCC"
                     /label="SfiI"
                     /note="Geneious type: restriction site"
     promoter        complement(5713..5817)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     complement(5735..5764)
                     /created_by="primer3"
                     /%GC="36.7"
                     /Tm="62.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CATGAGACAATAACCCTGATAAATGCTTCA"
                     /Transferred_From="pOPIN-R3"
                     /Transferred_Similarity="100.00%"
                     /label="pOPIN-R3"
     primer_bind     5749..5775
                     /created_by="primer3"
                     /%GC="44.4"
                     /Tm="62.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.7"
                     /Sequence="GGGTTATTGTCTCATGAGCGGATACAT"
                     /Transferred_From="pTARp75-R1"
                     /Transferred_Similarity="100.00%"
                     /label="pTARp75-R1"
     primer_bind     5828..5845
                     /created_by="primer3"
                     /%GC="55.6"
                     /Tm="56.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGAAAAGTGCCACCTGAC"
                     /Transferred_From="pBR1"
                     /Transferred_Similarity="100.00%"
                     /label="pBR1"
     rep_origin      5844..25
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN      
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcatgatg ctcaagtcta tcacagaaag ctttgccaca
     1021 gcaatccatg gcttgaaagt gggacacctg acagatcgtg ttattcagag gagcaagagg
     1081 atgattctag acactctggg tgctgggttc ctgggaacca ctacggaagt gtttcacata
     1141 gccagccaat atagcaagat ctacagttcc aacatatcca gcactgtttg gggtcagcca
     1201 gacatcaggc tcccgcccac atatgctgct tttgtgaacg gtgtggctat tcactccatg
     1261 gattttgatg acacgtggca ccctgccacc cacccttctg gggctgtcct tcctgtcctc
     1321 acagctttag cagaagccct gccaaggagt ccaaagtttt ctggccttga cctgctgctg
     1381 gctttcaatg ttggtattga agtgcaaggc cgattactgc atttcgccaa ggaggccagt
     1441 gacatgccaa agagattcca tcccccttcc gtggtaggaa cgttgggtag tgctgctgct
     1501 gcatccaagt ttttaggact tagctcgaca aagtgccgag aagctctggc cattgctgtt
     1561 tcccatgctg gggcacccat ggccaatgct gccacccaga ccaagcccct ccacattggc
     1621 aatgctgcca agcatgggat agaagctgca tttttggcaa tgttgggtct ccaaggaaac
     1681 aagcaggtct tggacttgga ggcaggattt ggggcctttt atgccaacta ttccccaaaa
     1741 gtccttccaa gcatagcttc ctacagttgg ctgctggacc agcaggacgt ggcctttaag
     1801 cgttttcctg cacatttatc tacccactgg gtggcagacg cagctgcatc tgtgagaaag
     1861 caccttgtag cagagagagc cctgcttcca actgactaca ttaagagaat tgtgctcagg
     1921 ataccaaatg tccagtatgt aaacaggccc tttccagttt cggagcatga agcccgtcat
     1981 tcattccagt atgtggcctg tgccatgctg cttgatggtg gcatcactgt cccctcattc
     2041 catgaatgcc agatcaacag gccacaggtg agagagctgc tcagtaaggt ggagctggag
     2101 taccctccgg acaacttgcc aagcttcaac atactgtact gtgaaataag tgtcaccctc
     2161 aaggatggag ccaccttcac agatcgctct gataccttct atgggcactg gagaaaacca
     2221 ctgagccagg aggacctaga ggaaaagttc agagccaatg cctccaagat gctgtcctgg
     2281 gacacagtgg aaagccttat aaagatagtc aaaaatctag aagacctaga agactgttct
     2341 gtgttaacta cacttctcaa aggaccctct ccaccagagg tagcttcaaa ctctccagca
     2401 tgtaataatt ctatcacaaa tctctccaag cggccgctcg agcagaaact catctcagaa
     2461 gaggatctgg cagcaaatga tatcctggat tacaaggatg acgacgataa ggtttaaacg
     2521 gccggccgcg gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc
     2581 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     2641 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     2701 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     2761 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     2821 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     2881 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     2941 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     3001 acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca
     3061 ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag
     3121 ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt
     3181 gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3301 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3961 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg
     4081 ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg
     4141 gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg
     4201 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
     4261 caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     4321 tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat
     4381 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     4441 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     4501 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     4561 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     4621 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc gatccgccac
     4681 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     4741 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag
     4801 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     4861 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     4921 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     4981 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     5041 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     5101 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga
     5161 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     5221 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     5281 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     5341 ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     5401 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     5461 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     5521 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     5581 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     5641 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct
     5701 ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5821 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5881 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5941 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6001 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6061 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6121 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6181 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6241 tgagctgatt taacaaaaat ttaacgcgaa tttt
//
