LOCUS       pCDH-EF1-N-cG-Neo        9347 bp    DNA     circular     26-JAN-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|-12131527|
COMMENT     VNTDBDATE|-12132952|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    5442..6032
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    6409..6540
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1810..1928
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    6104..6337
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(7883..8743)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1809
                     /vntifkey="21"
                     /label=env
     misc_feature    7065..7738
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    1961..3209
                     /vntifkey="21"
                     /label=EF-1\promoter
     misc_feature    3222..3740
                     /vntifkey="21"
                     /label=Nluc
     misc_feature    3222..3734
                     /vntifkey="21"
                     /label=Nluc_ORF
     misc_feature    3807..4562
                     /vntifkey="21"
                     /label=copGFP
     misc_feature    3741..3806
                     /vntifkey="21"
                     /label=P2A\peptide
     misc_feature    4569..4634
                     /vntifkey="21"
                     /label=P2A
     misc_feature    4635..5426
                     /vntifkey="21"
                     /label=Neo
     misc_feature    4553..4589
                     /vntifkey="21"
                     /label=5-P2A
BASE COUNT     2238 a      2340 c      2569 g      2200 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca 
     1921 aaattttatc gatatcgata gctttgcaaa gatggataaa gttttaaaca gagaggaatc 
     1981 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc 
     2041 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt 
     2101 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc 
     2161 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg 
     2221 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg 
     2281 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc 
     2341 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt 
     2401 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc 
     2461 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag 
     2521 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa 
     2581 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg 
     2641 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa 
     2701 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt 
     2761 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa 
     2821 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag 
     2881 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt 
     2941 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt 
     3001 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg 
     3061 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg 
     3121 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt 
     3181 ttcttccatt tcaggtgtcg tgaggaattg gatccgccac catggtcttc acactcgaag 
     3241 atttcgttgg ggactggcga cagacagccg gctacaacct ggaccaagtc cttgaacagg 
     3301 gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt aactccgatc caaaggattg 
     3361 tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt catcatcccg tatgaaggtc 
     3421 tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg 
     3481 atcatcactt taaggtgatc ctgcactatg gcacactggt aatcgacggg gttacgccga 
     3541 acatgatcga ctatttcgga cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga 
     3601 tcactgtaac agggaccctg tggaacggca acaaaattat cgacgagcgc ctgatcaacc 
     3661 ccgacggctc cctgctgttc cgagtaacca tcaacggagt gaccggctgg cggctgtgcg 
     3721 aacgcattct ggcgaagctt ggaagcggag ctactaactt cagcctgctg aagcaggctg 
     3781 gagacgtgga ggagaaccct ggacctatgg agagcgacga gagcggcctg cccgccatgg 
     3841 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 
     3901 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc 
     3961 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 
     4021 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 
     4081 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 
     4141 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 
     4201 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 
     4261 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 
     4321 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 
     4381 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 
     4441 agctgggcat cgtggagtac cagcacgcct tcaagacccc cattgccttc gccagatccc 
     4501 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 
     4561 gcgtcgacgg aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg 
     4621 agaaccctgg acctatggct gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     4681 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     4741 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     4801 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     4861 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     4921 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     4981 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     5041 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     5101 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     5161 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     5221 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     5281 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     5341 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     5401 tctatcgcct tcttgacgag ttcttctgag aattcgtcga caatcaacct ctggattaca 
     5461 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     5521 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     5581 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
     5641 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
     5701 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
     5761 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
     5821 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga 
     5881 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
     5941 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
     6001 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag 
     6061 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 
     6121 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 
     6181 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 
     6241 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 
     6301 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 
     6361 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 
     6421 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     6481 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     6541 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     6601 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     6661 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat 
     6721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 
     6781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 
     6841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 
     6901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     6961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     7021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     7081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     7141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     7201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     7261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     7321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     7381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     7441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     7501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     7561 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     7621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     7681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     7741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     7801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     7861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     7921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     7981 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     8041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     8101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     8161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     8221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     8281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     8341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     8401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     8461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     8521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     8581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     8641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     8701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     8761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     8821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 
     8881 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 
     8941 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 
     9001 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 
     9061 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 
     9121 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 
     9181 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 
     9241 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 
     9301 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg 
//
