LOCUS       pCDH-EF1-N-cG-Blast        8948 bp    DNA     circular     25-JAN-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12131558|
COMMENT     VNTDBDATE|-12131558|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCDH-EF1-N-cG-Blast|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    5043..5633
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    6010..6141
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1810..1928
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    5705..5938
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(7484..8344)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1809
                     /vntifkey="21"
                     /label=env
     misc_feature    6666..7339
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    1961..3209
                     /vntifkey="21"
                     /label=EF-1\promoter
     misc_feature    3222..3740
                     /vntifkey="21"
                     /label=Nluc
     misc_feature    3222..3734
                     /vntifkey="21"
                     /label=Nluc_ORF
     misc_feature    3807..4562
                     /vntifkey="21"
                     /label=copGFP
     misc_feature    3741..3806
                     /vntifkey="21"
                     /label=P2A\peptide
     misc_feature    4569..4631
                     /vntifkey="21"
                     /label=T2A\Peptide
     bHLH_domain     4632..5027
                     /vntifkey="1"
                     /label=BSD
     misc_feature    3200..3236
                     /vntifkey="21"
                     /label=5B-Nluc
     misc_feature    4549..4568
                     /vntifkey="21"
                     /label=3S-copGFP
     misc_feature    4553..4585
                     /vntifkey="21"
                     /label=5-T2A-BSD
     misc_feature    5016..5046
                     /vntifkey="21"
                     /label=3E-BSD
BASE COUNT     2183 a      2216 c      2435 g      2114 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca 
     1921 aaattttatc gatatcgata gctttgcaaa gatggataaa gttttaaaca gagaggaatc 
     1981 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc 
     2041 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt 
     2101 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc 
     2161 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg 
     2221 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg 
     2281 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc 
     2341 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt 
     2401 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc 
     2461 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag 
     2521 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa 
     2581 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg 
     2641 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa 
     2701 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt 
     2761 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa 
     2821 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag 
     2881 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt 
     2941 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt 
     3001 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg 
     3061 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg 
     3121 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt 
     3181 ttcttccatt tcaggtgtcg tgaggaattg gatccgccac catggtcttc acactcgaag 
     3241 atttcgttgg ggactggcga cagacagccg gctacaacct ggaccaagtc cttgaacagg 
     3301 gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt aactccgatc caaaggattg 
     3361 tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt catcatcccg tatgaaggtc 
     3421 tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg 
     3481 atcatcactt taaggtgatc ctgcactatg gcacactggt aatcgacggg gttacgccga 
     3541 acatgatcga ctatttcgga cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga 
     3601 tcactgtaac agggaccctg tggaacggca acaaaattat cgacgagcgc ctgatcaacc 
     3661 ccgacggctc cctgctgttc cgagtaacca tcaacggagt gaccggctgg cggctgtgcg 
     3721 aacgcattct ggcgaagctt ggaagcggag ctactaactt cagcctgctg aagcaggctg 
     3781 gagacgtgga ggagaaccct ggacctatgg agagcgacga gagcggcctg cccgccatgg 
     3841 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 
     3901 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc 
     3961 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 
     4021 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 
     4081 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 
     4141 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 
     4201 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 
     4261 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 
     4321 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 
     4381 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 
     4441 agctgggcat cgtggagtac cagcacgcct tcaagacccc cattgccttc gccagatccc 
     4501 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 
     4561 gcgtcgacgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga 
     4621 atcctggccc aatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa 
     4681 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct 
     4741 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg 
     4801 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg 
     4861 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc 
     4921 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg 
     4981 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gaattcgtcg 
     5041 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 
     5101 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 
     5161 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 
     5221 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 
     5281 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 
     5341 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     5401 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 
     5461 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 
     5521 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 
     5581 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct 
     5641 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 
     5701 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg 
     5761 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 
     5821 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 
     5881 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 
     5941 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 
     6001 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     6061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     6121 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 
     6181 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
     6241 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 
     6301 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 
     6361 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 
     6421 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 
     6481 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
     6541 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     6601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     6661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     6721 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     6781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     6841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     6901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     6961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     7021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     7081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     7141 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 
     7201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     7261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     7321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     7381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     7441 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     7501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     7561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     7621 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     7681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     7741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     7801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     7861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     7921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     7981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     8041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     8101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     8161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     8221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     8281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     8341 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     8401 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     8461 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 
     8521 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 
     8581 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 
     8641 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 
     8701 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 
     8761 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 
     8821 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 
     8881 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 
     8941 ccaagctg 
//
