LOCUS       Exported                8870 bp ds-DNA     circular SYN 20-OCT-2008
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 25, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
COMMENT     ApEinfo:methylated:0
            ORIGIN
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=Vector 
                     1045A-attB-white-U63-eve-gRNA-U61-hid-gRNA-gBlock"
     promoter        623..642
                     /note="T7 Promoter"
     gene            667..4802
                     /label=White dominant eye marker
                     /note="White dominant eye marker"
     misc_feature    4699..4725
                     /label=nonstandard type: PCR_primer
                     /note="attBseqF"
                     /note="/current=0"
     misc_binding    4809..4842
                     /note="loxP"
     misc_feature    4856..4877
                     /label=nonstandard type: PCR_primer
                     /note="attB8seqF - Primer 190"
                     /note="/current=0"
     misc_feature    4895..5322
                     /label=U6-3 Promoter
                     /note="U6-3 Promoter"
     primer_bind     4895..4919
                     /label=Primer 1
     primer_bind     complement(4988..5012)
                     /label=Primer 6
     primer_bind     complement(5228..5247)
                     /label=Primer 5
     primer_bind     5228..5247
                     /label=Primer 4
     misc_feature    5286..6361
                     /label=gBlock
                     /note="gBlock"
     primer_bind     5286..5358
                     /label=Primer 8
     primer_bind     5299..5358
                     /label=Primer 14
     misc_feature    5323..5340
                     /label=eve target
                     /note="eve target"
     misc_RNA        5341..5416
                     /label=scaffold
                     /note="scaffold"
     primer_bind     5341..5363
                     /label=Primer 13
     primer_bind     5341..5363
                     /label=Primer 12
     primer_bind     5341..5358
                     /label=Primer 7
     terminator      5417..5422
     promoter        5431..6227
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     primer_bind     5618..5635
                     /label=Primer 19
     primer_bind     complement(5618..5635)
                     /label=Primer 18
     primer_bind     6027..6044
                     /label=Primer 17
     primer_bind     complement(6027..6044)
                     /label=Primer 16
     primer_bind     6209..6268
                     /label=Primer 13
     primer_bind     complement(6210..6239)
                     /label=Primer 11
     primer_bind     complement(6210..6227)
                     /label=Primer 10
     primer_bind     6221..6263
                     /label=Primer 8
     misc_feature    6228..6245
                     /label=gRNA target
                     /note="gRNA target"
     misc_RNA        6246..6321
                     /label=scaffold
                     /note="scaffold"
     primer_bind     6246..6268
                     /label=Primer 12
     primer_bind     6246..6263
                     /label=Primer 7
     primer_bind     complement(6311..6351)
                     /label=Primer 15
     terminator      6322..6327
     misc_binding    6373..6657
                     /note="attB site"
     misc_feature    complement(6379..6402)
                     /label=nonstandard type: PCR_primer
                     /note="attBseqR Primer 191"
                     /note="/current=0"
     primer_bind     complement(6442..6464)
                     /label=Primer 9
     promoter        6681..6700
                     /note="T3 promoter"
     misc_feature    7885..8742
                     /label=Ampicillin R
                     /note="Ampicillin R"
     misc_feature    7885..7904
     misc_feature    8719..8742
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccgggaat
     4861 tgggaattgg gcaatattta aatggcggcg cgccgaattc ttttttgctc acctgtgatt
     4921 gctcctactc aaatacaaaa acatcaaatt ttctgtcaat aaagcatatt tatttatatt
     4981 tattttacag gaaagaattc cttttaaagt gtattttaac ctataatgaa aaacgattaa
     5041 aaaaaataca taaaataatt cgaaaatttt tgaatagccc aggttgataa aaattcattt
     5101 catacgtttt ataacttatg cccctaagta ttttttgacc atagtgtttc aattctacat
     5161 taattttaca gagtagaatg aaacgccacc tactcagcca agaggcgaaa aggttagctc
     5221 gccaagcaga gagggcgcca gtgctcacta ctttttataa ttctcaactt ctttttccag
     5281 actcagttcg tatatataga cctattttca atttaacgtc ggatcgtgcg gtgctgagag
     5341 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     5401 ggcaccgagt cggtgctttt ttgtttaaac attttcaacg tcctcgatag tatagtggtt
     5461 agtatccccg cctgtcacgc gggagaccgg ggttcaattc cccgtcgggg agaatctgtg
     5521 attctttttt tttttctttt actttgttat ataaacaatt tttgttttaa ttgaatctaa
     5581 tttgccattg cttttaggaa tctcaggcat ccagcaagcg tttgtccgcc gaatcgccca
     5641 tcagtgaaga agatcctgtg gcggctacga aaatctcccc ggccatgtcg gcctccacct
     5701 ccagcgaaaa acccatcagc gagctggcca cctctgtgct gacccaccgc tttccagact
     5761 ccacctcctc acccggcgaa catggccttg gacgaatgca gttgtcgatc cgctacagcg
     5821 cccagcgtca aaaactagac gtgaccatac acaaaatcca gaagatacca cttcgcgatc
     5881 ccagcaatat ccccgatccg tatgttaagc tgtatctgtt gcctggacgc accaaggagt
     5941 cgaaacgcaa gacgagcgtg atcaaggaca actgcaaccc cgtctacgat gcatcctttg
     6001 agtacctgat ttccattgcc gaactcaggc agacggaact ggaggtgacg gtgtgcaccc
     6061 aaaagggatt cctatccggc ggtagtccca tcattggcat ggtaggtacc cgaaagcaac
     6121 cccttagtta cagacacagc gcgtacgtcc ttcgcatcct tatgattccc aagtacatat
     6181 tctgcaagag tacagtatat ataggaaaga tatccgggtg aacttcggca catgcacgtg
     6241 catgagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     6301 aaagtggcac cgagtcggtg cttttttaat taaggaacgc ggccgcaacg gtacctctag
     6361 agatccacta gtgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg
     6421 cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca tcatgatgaa
     6481 cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc
     6541 ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg
     6601 tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc acggcgggca tgtcgacact
     6661 agttctagcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
     6721 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6781 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6841 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6901 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
