LOCUS Exported 2556 bp ds-DNA circular SYN 24-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2556) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 24, 2018 from SnapGene Viewer 4.2.6 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2556 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 3..8 /label=US BsaI site misc_feature 18..25 /label=AscI site rep_origin 27..876 /label=I4L /note="Left homology arm for site I4" misc_feature 877..906 /label=ConLS' overhang misc_feature complement(908..913) /label=DS BsaI site CDS complement(917..1790) /label=CamR rep_origin 1791..2554 /label=ColE1 ORIGIN 1 tcggtctcac cctttcaggc gcgccgggtg ttgcattagt atcgccgtgg atgaagatga 61 aggtggaata gacgttaccc atagataccc gaatgagtat ctgattgagg atttttgact 121 tacttacttc ttcgagatac actactctgc cgaaatttcg gactggaatg cgtcaaagtg 181 aatccacttt gcaggaaaac gcaggaaaaa cggattcgaa aacgcaacaa aaaaaaaaaa 241 attatacata ataaaatttg aatcaaagca acaagttcac gaatccatta aagcagtcag 301 aatccaggaa aaaaaagaaa cagagccaga aaaaaggcag caacgaatca caaccacagc 361 gtattgcgtc agtggaaatt aattaccttg gtatttccaa tgctgtgtac aaccaaaatt 421 tccacatagt tagttgattc gcatttccct ggtaaccgcc ctcggcaacc tccctcggaa 481 acttccctcc agagtaactt cccccagagt tatagagata tagagttata gtagagatat 541 agagctatag atagattgat tcttgttgta gcatttattc cgttaaactt ctgcggctaa 601 ttttgcctag cttggccttt tttttttgtt tttcgagctt tcaaagacaa atggacattc 661 ttacataata tattatcata tacaccgaga tcggtgggtc agagcctgta gggatggaca 721 ttttccttct agactcgcct tccagacctt tttttggttt gtttctctct ccgtggaact 781 cgctttcttt ttcgccggtg atctcgaaac gtaatcgata tgtattccat tccattcgct 841 attttttttg ttccataaac catgttctta tgaacagaat tcgcatctag actgatgaga 901 cgaacgtgag acctagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat 961 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata 1021 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 1081 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc 1141 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 1201 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac 1261 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 1321 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 1381 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 1441 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga 1501 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc 1561 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca 1621 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc 1681 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt 1741 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa 1801 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 1861 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 1921 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 1981 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 2041 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 2101 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 2161 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 2221 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 2281 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 2341 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 2401 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 2461 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 2521 tcctgcgtta tcccctgatt ctgtggataa ccgtag //