LOCUS       Exported                2925 bp ds-DNA     circular SYN 22-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 22, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA [LOCUS]: Exported [ACCESSION]: 
            . [SOURCE]: 1..2926 [CDS]: complement(1287..2160)
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3..8
                     /label=US BsaI site
                     /note="label=US BsaI site - 3..8"
     rep_origin      14..1271
                     /label=C2
                     /note="label=C2;KmARS/CEN5 - note=""ARS/CEN from chromosome
                     5 of Kluyveromyces marxianus homologous to - ARS1 from 
                     strain ATCC 12424"" - 14..1271"
     misc_feature    complement(1277..1282)
                     /label=DS BsaI site
                     /note="label=DS BsaI site - complement(1278..1283)"
     CDS             complement(1286..2159)
                     /label=CamR
                     /note="label=CamR - complement(1287..2160)"
     rep_origin      2160..2923
                     /label=ColE1
                     /note="label=ColE1 - 2161..2924"
ORIGIN
        1 tcggtctcag agtgagctcc tttcatttct gataaaagta aggtttctct atttatcttt
       61 tcacccacat tatccttcga agtacgtata caatattagt tcaacgtaaa aacaaaactt
      121 actgtaaata tgcgtaaaaa aaatctatta aattcatagc agtttcaagg aaagagaacc
      181 attatggtct ggtcacgtgt gtataaatta ttaattttaa cactatataa tttattattt
      241 ttttattttg aagtttagag taattttagt agtattttat attttaaata aatatatttt
      301 aaatttttac ttaatatttt attatttttt aatacaatgt ttttatttaa aacaaaatta
      361 taaattaaaa tgttgttccg aaagtaaaat atattttatg gtttttacaa aaataaatta
      421 tttttaatgt atttttttaa ttatattttt gtatgtaatt atatccacag gtattatgtt
      481 gaatttagct gttttagttt acctgtgtgg tactatgagt tttttgcctc tcaaaagcta
      541 ttttttagaa ctctctctcc tcttagaaat aggtggtgtt gcggttgact tttaacgata
      601 tatcattttc aatttattta ttttaaagtg acatagagag attcctttta attttttaat
      661 ttttattttc aataatttta aaaatggggg acttttagat tggaacaaaa tgaaaaatat
      721 ctgttatacg tgcaactgaa ttttactgac cttaaaggac tatctcgaac ttggttcgga
      781 aatccttgaa atgattgata ttttggtgga ttttctctga ttttcaaaca agtagtattt
      841 tatttaatat ttattatatt ttttacattt ttttatattt ttttattgtt tggaagggaa
      901 agcaacaatt actttcaaaa tatataaatt aaactgaaat acttaataag agacaaataa
      961 cattcaagaa tcaaatactg ggttattaat caaaagatct ctctacatgc acccaaattc
     1021 actatttaaa tttactatac cactgacaga atatatgaac ccagattaag tagccagagg
     1081 ctcttccact atattgagta tatagcctta catattttct gcgcataatt ttctggatgt
     1141 aaaataaaca aaaatagtta gtttgtagtt atgaaaaaag gcttttggaa aatgcggaat
     1201 acgtgttatt taaggttaat caacaaaacg catatccata gtggatagtt ggataaaact
     1261 tcaattgatc cccgatgaga cctagaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1321 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1381 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1441 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1501 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1561 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
     1621 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1681 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1741 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1801 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1861 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1921 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1981 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     2041 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     2101 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
     2161 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2221 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2341 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2821 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2881 catgttcttt cctgcgttat cccctgattc tgtggataac cgtag
//
