LOCUS       Exported                2530 bp ds-DNA     circular SYN 22-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 22, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA [LOCUS]: Exported [ACCESSION]: 
            . [SOURCE]: 1..2534 [CDS]: complement(895..1768)
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3..8
                     /label=US BsaI site
                     /note="label=US BsaI site - 3..8"
     rep_origin      14..862
                     /label=I4R
                     /note="label=I4R - note=""Right homology arm for site I4"" 
                     - 14..862"
     misc_feature    complement(865..872)
                     /label=AscI site
                     /note="label=AscI site - complement(865..872)"
     misc_feature    complement(882..887)
                     /label=DS BsaI site
                     /note="label=DS BsaI site - complement(886..891)"
     CDS             complement(891..1764)
                     /label=CamR
                     /note="label=CamR - complement(895..1768)"
     rep_origin      1765..2528
                     /label=ColE1
                     /note="label=ColE1 - 1769..2532"
ORIGIN
        1 tcggtctcag agtttcacaa tggagggagt gaaatctaaa agtggaaaaa aaaaggggag
       61 gcgaaaaaca aaaaaaaaaa aaagaacgaa ccgaactatg tccacaggag cagaagcaga
      121 agctgcggca gcagcttctg cggctttgaa agaggaaacg cgtagatgaa agaatgatcc
      181 aagcccggct ttccctctgt gcgcggattg ttattttttt agacttattt ctatgtttgt
      241 tttggaccta atataactta gtcatgaatc ctggttgact cgcgcgcatc tttttccact
      301 ctgcgatgat tcacagtgca gcgtgcagct cagctccagc agcagtacca gcggcagctc
      361 caacagtgga gccttccttt cacgaacgga atagaaacag gaaggaaaaa aatagaccaa
      421 caagaggacc catccgcgac ttctaatttt tggcgcatga accgcgctgt tctacccgcc
      481 cgtccgtcct catccctccc ccccaggaaa acaggaaatt gggaaaccta ggtgcgtgca
      541 ttttcgtttt gggcgatgat tgacaatgag acttagtccg cttggttggg tttcgtttca
      601 aaaacaaggt ttggaagaag atcttgggag agaaagaccg agagatcttg accagggcga
      661 agacgaccag tagcttttgg tatagccggg ataagcttca ggcaccctag ggttgtataa
      721 gagttgtatg caaggatgtg cgcaagggtt tctgaagcag gatggaaatg tggtgtgttt
      781 ttttgtagag caattgcctt aaagtcgggg attacgccga gacagcagct tgacggttcc
      841 gtggtaatat gtgtccggca acttggcgcg cctcagccga tgagacctag accaataaaa
      901 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      961 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1021 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     1081 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1141 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     1201 gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa
     1261 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1321 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1381 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1441 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1501 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1561 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     1621 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     1681 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1741 ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2521 ataaccgtag
//
