LOCUS       pKD278                  5741 bp ds-DNA     circular     13-MAR-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2079..3038
                     /label="LacI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4893..5084
                     /label="HisKA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3302..3363
                     /label="tac promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4671..4730
                     /label="TM"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    3334..3356
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1956..3038
                     /label="lacI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3364..3398
                     /label="sRBS (1,000)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      complement(948..1053)
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     terminator      3039..3143
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    5399..5404
                     /label="BsaI out"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        1..148
                     /label="specR_promoter_from_pKD13"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3432..3488
                     /label="TM"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3399..5558
                     /label="SO_2192"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    149..940
                     /label="SpecR"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    3531..4313
                     /label="CBM9"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5217..5555
                     /label="HATPase_c"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4920..4922
                     /label="H508"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(1168..1855)
                     /label="p15A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3197..3301
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5568..5696
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    4671..4874
                     /label="HAMP"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA
       61 AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGATCTGA
      121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GAGGGAAGCG GTGATCGCCG AAGTATCGAC
      181 TCAACTATCA GAGGTAGTTG GCGTCATCGA GCGCCATCTC GAACCGACGT TGCTGGCCGT
      241 ACATTTGTAC GGCTCCGCAG TGGATGGCGG CCTGAAGCCA CACAGTGATA TTGATTTGCT
      301 GGTTACGGTG ACCGTAAGGC TTGATGAAAC AACGCGGCGA GCTTTGATCA ACGACCTTTT
      361 GGAAACTTCG GCTTCCCCTG GAGAGAGCGA GATTCTCCGC GCTGTAGAAG TCACCATTGT
      421 TGTGCACGAC GACATCATTC CGTGGCGTTA TCCAGCTAAG CGCGAACTGC AATTTGGAGA
      481 ATGGCAGCGC AATGACATTC TTGCAGGTAT CTTCGAGCCA GCCACGATCG ACATTGATCT
      541 GGCTATCTTG CTGACAAAAG CAAGAGAACA TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA
      601 GGAACTCTTT GATCCGGTTC CTGAACAGGA TCTATTTGAG GCGCTAAATG AAACCTTAAC
      661 GCTATGGAAC TCGCCGCCCG ACTGGGCTGG CGATGAGCGA AATGTAGTGC TTACGTTGTC
      721 CCGCATTTGG TACAGCGCAG TAACCGGCAA AATCGCGCCG AAGGATGTCG CTGCCGACTG
      781 GGCAATGGAG CGCCTGCCGG CCCAGTATCA GCCCGTCATA CTTGAAGCTA GACAGGCTTA
      841 TCTTGGACAA GAAGAAGATC GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG AATTTGTCCA
      901 CTACGTGAAA GGCGAGATCA CCAAGGTAGT CGGCAAATAA GAGCTCGCTT GGACTCCTGT
      961 TGATAGATCC AGTAATGACC TCAGAACTCC ATCTGGATTT GTTCAGAACG CTCGGTTGCC
     1021 GCCGGGCGTT TTTTATTGGT GAGAATCCAA GCACTAGTAA CAACTTATAT CGTATGGGGC
     1081 TGACTTCAGG TGCTACATTT GAAGAGATAA ATTGCACTGA AATCTAGTAA TATTTTATCT
     1141 GATTAATAAG ATGATCTTCT TGAGATCGTT TTGGTCTGCG CGTAATCTCT TGCTCTGAAA
     1201 ACGAAAAAAC CGCCTTGCAG GGCGGTTTTT CGAAGGTTCT CTGAGCTACC AACTCTTTGA
     1261 ACCGAGGTAA CTGGCTTGGA GGAGCGCAGT CACCAAAACT TGTCCTTTCA GTTTAGCCTT
     1321 AACCGGCGCA TGACTTCAAG ACTAACTCCT CTAAATCAAT TACCAGTGGC TGCTGCCAGT
     1381 GGTGCTTTTG CATGTCTTTC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     1441 CGGTCGGACT GAACGGGGGG TTCGTGCATA CAGTCCAGCT TGGAGCGAAC TGCCTACCCG
     1501 GAACTGAGTG TCAGGCGTGG AATGAGACAA ACGCGGCCAT AACAGCGGAA TGACACCGGT
     1561 AAACCGAAAG GCAGGAACAG GAGAGCGCAC GAGGGAGCCG CCAGGGGGAA ACGCCTGGTA
     1621 TCTTTATAGT CCTGTCGGGT TTCGCCACCA CTGATTTGAG CGTCAGATTT CGTGATGCTT
     1681 GTCAGGGGGG CGGAGCCTAT GGAAAAACGG CTTTGCCGCG GCCCTCTCAC TTCCCTGTTA
     1741 AGTATCTTCC TGGCATCTTC CAGGAAATCT CCGCCCCGTT CGTAAGCCAT TTCCGCTCGC
     1801 CGCAGTCGAA CGACCGAGCG TAGCGAGTCA GTGAGCGAGG AAGCGGAATA TATCCCGAAG
     1861 CGGCATGCAT TTACGTTGAC ACCATCGAAT GGTGCAAAAC CTTTCGCGGT ATGGCATGAT
     1921 AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG TTATACGATG
     1981 TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC
     2041 ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC
     2101 CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT
     2161 CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC
     2221 AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG
     2281 CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG
     2341 ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG
     2401 ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC GGTACGCGAC
     2461 TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT
     2521 TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC
     2581 AAATTCAGCC GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA
     2641 CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA
     2701 TGGCGCTGGG CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGATATCT
     2761 CGGTAGTGGG ATACGACGAT ACCGAAGACA GCTCATGTTA TATCCCGCCG TCAACCACCA
     2821 TCAAACAGGA TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC
     2881 AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA
     2941 CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC
     3001 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGG CATCAAATAA AACGAAAGGC
     3061 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG
     3121 TAGGACAAAT CCGCCGCCCT AGACCTAGGC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
     3181 TCAAAGGCGG TAATACGTAA ATCACTGCAT AATTCGTGTC GCTCAAGGCG CACTCCCGTT
     3241 CTGGATAATG TTTTTTGCGC CGACATCATA ACGGTTCTGG CAAATATTCT GAAATGAGCT
     3301 GTTGACAATT AATCATCGGC TCGTATAATG TGTGGAATTG TGAGCGGATA ACAATTTCAC
     3361 ACACACTATT CTCATCTAAA TTTATCAAAA CGCCTACAat gacacgtttt cctttcggtc
     3421 ttcgcactaa agtgattggc ctgagcctat ttttgctctg cctaccttgg ctggggtacc
     3481 aatatgtgtg ggagatggaa aaatacctgc gccacggcca agaacgtacc ttagagggca
     3541 caacccaagc gctagcgacc gccctgcacg agcggcccaa acttttcgat ggccaagcca
     3601 gttttttaaa acaggttgaa aagagccgtg acctctatgc ttacccctta gccggagcca
     3661 ttctgctcga tggcaaactc aatgaatggc aggaatatca acatcgattt atccaatacg
     3721 gtgaagccaa tcagattatg cgcgccgatc ccagtgcgcc gctcacctta agttttaccc
     3781 atatggtggg ccagtacgat ggctatctgt atgctttttt tcaggtggtg gaccccaatg
     3841 tggtgtttcg tggcaaaaat gccctaagtg tggataggaa cgatcatcta tcaattgcga
     3901 cccttgcccc cgacaataaa tttaagcgtt atatcgttgc caccactcag cccggttgga
     3961 tcagcgcctt tgaactgccc gccaacccga aacaaaccgt gccagtgaca cccgaggtgc
     4021 gcattcaagg acaatgggcc accacagatg tgggctacaa tatcgagctg cgtattcccc
     4081 ttgatatggt gggcagcaaa ctcgggtttg ccattgccga tgttaacgac agcaaatacc
     4141 gcgatgtcgc cgcagtggtt ggcacttccg ccttagatac cgtcgataag ctgggcacag
     4201 tgctggtccc ctcccctgaa attgaaaaca ttatcaaagg gatgagccac tacagctcgc
     4261 gtatttgggt aatagataaa catggccgcg tgttagcaaa atccggcgat atccgcgaca
     4321 gcaacagcgt gtggactcgc acccttgatg atgaaaacag caatggccta tggcagcggt
     4381 ttcagcgcga ttatctgcat ccgctctact ataaaatcct cactaaaccg ccgaaggatt
     4441 ttatcgactc tctgcgggat tcaaccgagc taaatggcag tcatattcaa aaagccctcg
     4501 ccggacaaca gacgtcgaca tggcgcttga cgcctgacaa taaggccgtg gtactcgcgg
     4561 cagccagccc aatttggatt gataacaatg tgatgggcgc ggtagtggct gaagaaacca
     4621 cccacggtat tcgcacccta cgtaatcgcg cgttggaaaa actgtttaac gtgatcctga
     4681 ccattatgag catgggcacc ttagcgctgt ttttcttcgc atcgagtatt tccaatcgca
     4741 ttcgtaagtt aagggacgaa gccgaaaatg ccatcgacag ccaaggtcgg gtgcgtaacc
     4801 atctaaaacc ctcaaaggcg cgggacgaga ttggcgactt atcccgcagt tttgccagca
     4861 ttgtaggacg tttaggccaa tacactcatt atttggagaa tatgtcgtcg cgcttatccc
     4921 atgagctacg tactcctgtt gcagtggtgc gttcatcttt agagcactta aatctgcaaa
     4981 ccttagataa agacacgcaa aagtatgtcg atcgcgccca agaaggcgta agccgtttaa
     5041 gcatgatttt gaataatatg agcgaggcga cgcgcctaga gcagagctta actcaggccg
     5101 atgtgactaa gtttccgcta tcccaagtgg tcagcggctg tatgcaaggc tatcaaatga
     5161 cctatcccga gcagcgtttt agcatcaaag tgcccgatca gccgttgatg atgcagggtg
     5221 tgccagaata catcgcccaa ttgatggata aacttatcgc caatgccatc gaattttgtc
     5281 atcaaggcag ttcgattgag gtcaccctca ctcaagtcgg taaacaagcc acattgctta
     5341 tcagcaactt agggccgcag ttgccggtgg atatgtcgga gcagatcttc gactcaatgg
     5401 tatcggtgag ggataatcaa acccaggaga aaccccacct tggcctaggt ttgtatattg
     5461 cacggctcgt gaccgagttt caccaaggga atatcttagc ccgcaaccgc agcgaaaatg
     5521 atggggtcga tatcctagtc accctgccgc tgtgctaaTA ATCTAGACCA GGCATCAAAT
     5581 AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA
     5641 CGCTCTCTAC TAGAGTCACA CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATAGGTA
     5701 CCCGGGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT T
//