LOCUS       pKD282                  4838 bp ds-DNA     circular     25-JUL-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3364..3398
                     /label="sRBS (1,000)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3399..4655
                     /label="SO_4427"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3302..3363
                     /label="tac promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    149..940
                     /label="SpecR"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     rep_origin      complement(1168..1855)
                     /label="p15A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4665..4793
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     terminator      3039..3143
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     promoter        1..148
                     /label="specR_promoter_from_pKD13"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2079..3038
                     /label="LacI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_binding    3334..3356
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      complement(948..1053)
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    3197..3301
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4050..4052
                     /label="His"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1956..3038
                     /label="lacI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA
       61 AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGATCTGA
      121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GAGGGAAGCG GTGATCGCCG AAGTATCGAC
      181 TCAACTATCA GAGGTAGTTG GCGTCATCGA GCGCCATCTC GAACCGACGT TGCTGGCCGT
      241 ACATTTGTAC GGCTCCGCAG TGGATGGCGG CCTGAAGCCA CACAGTGATA TTGATTTGCT
      301 GGTTACGGTG ACCGTAAGGC TTGATGAAAC AACGCGGCGA GCTTTGATCA ACGACCTTTT
      361 GGAAACTTCG GCTTCCCCTG GAGAGAGCGA GATTCTCCGC GCTGTAGAAG TCACCATTGT
      421 TGTGCACGAC GACATCATTC CGTGGCGTTA TCCAGCTAAG CGCGAACTGC AATTTGGAGA
      481 ATGGCAGCGC AATGACATTC TTGCAGGTAT CTTCGAGCCA GCCACGATCG ACATTGATCT
      541 GGCTATCTTG CTGACAAAAG CAAGAGAACA TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA
      601 GGAACTCTTT GATCCGGTTC CTGAACAGGA TCTATTTGAG GCGCTAAATG AAACCTTAAC
      661 GCTATGGAAC TCGCCGCCCG ACTGGGCTGG CGATGAGCGA AATGTAGTGC TTACGTTGTC
      721 CCGCATTTGG TACAGCGCAG TAACCGGCAA AATCGCGCCG AAGGATGTCG CTGCCGACTG
      781 GGCAATGGAG CGCCTGCCGG CCCAGTATCA GCCCGTCATA CTTGAAGCTA GACAGGCTTA
      841 TCTTGGACAA GAAGAAGATC GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG AATTTGTCCA
      901 CTACGTGAAA GGCGAGATCA CCAAGGTAGT CGGCAAATAA GAGCTCGCTT GGACTCCTGT
      961 TGATAGATCC AGTAATGACC TCAGAACTCC ATCTGGATTT GTTCAGAACG CTCGGTTGCC
     1021 GCCGGGCGTT TTTTATTGGT GAGAATCCAA GCACTAGTAA CAACTTATAT CGTATGGGGC
     1081 TGACTTCAGG TGCTACATTT GAAGAGATAA ATTGCACTGA AATCTAGTAA TATTTTATCT
     1141 GATTAATAAG ATGATCTTCT TGAGATCGTT TTGGTCTGCG CGTAATCTCT TGCTCTGAAA
     1201 ACGAAAAAAC CGCCTTGCAG GGCGGTTTTT CGAAGGTTCT CTGAGCTACC AACTCTTTGA
     1261 ACCGAGGTAA CTGGCTTGGA GGAGCGCAGT CACCAAAACT TGTCCTTTCA GTTTAGCCTT
     1321 AACCGGCGCA TGACTTCAAG ACTAACTCCT CTAAATCAAT TACCAGTGGC TGCTGCCAGT
     1381 GGTGCTTTTG CATGTCTTTC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     1441 CGGTCGGACT GAACGGGGGG TTCGTGCATA CAGTCCAGCT TGGAGCGAAC TGCCTACCCG
     1501 GAACTGAGTG TCAGGCGTGG AATGAGACAA ACGCGGCCAT AACAGCGGAA TGACACCGGT
     1561 AAACCGAAAG GCAGGAACAG GAGAGCGCAC GAGGGAGCCG CCAGGGGGAA ACGCCTGGTA
     1621 TCTTTATAGT CCTGTCGGGT TTCGCCACCA CTGATTTGAG CGTCAGATTT CGTGATGCTT
     1681 GTCAGGGGGG CGGAGCCTAT GGAAAAACGG CTTTGCCGCG GCCCTCTCAC TTCCCTGTTA
     1741 AGTATCTTCC TGGCATCTTC CAGGAAATCT CCGCCCCGTT CGTAAGCCAT TTCCGCTCGC
     1801 CGCAGTCGAA CGACCGAGCG TAGCGAGTCA GTGAGCGAGG AAGCGGAATA TATCCCGAAG
     1861 CGGCATGCAT TTACGTTGAC ACCATCGAAT GGTGCAAAAC CTTTCGCGGT ATGGCATGAT
     1921 AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG TTATACGATG
     1981 TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC
     2041 ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC
     2101 CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT
     2161 CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC
     2221 AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG
     2281 CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG
     2341 ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG
     2401 ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC GGTACGCGAC
     2461 TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT
     2521 TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC
     2581 AAATTCAGCC GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA
     2641 CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA
     2701 TGGCGCTGGG CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGATATCT
     2761 CGGTAGTGGG ATACGACGAT ACCGAAGACA GCTCATGTTA TATCCCGCCG TCAACCACCA
     2821 TCAAACAGGA TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC
     2881 AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA
     2941 CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC
     3001 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGG CATCAAATAA AACGAAAGGC
     3061 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG
     3121 TAGGACAAAT CCGCCGCCCT AGACCTAGGC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
     3181 TCAAAGGCGG TAATACGTAA ATCACTGCAT AATTCGTGTC GCTCAAGGCG CACTCCCGTT
     3241 CTGGATAATG TTTTTTGCGC CGACATCATA ACGGTTCTGG CAAATATTCT GAAATGAGCT
     3301 GTTGACAATT AATCATCGGC TCGTATAATG TGTGGAATTG TGAGCGGATA ACAATTTCAC
     3361 ACAACCCTAT TTTATTCTTA CTTCCCTTAG AGAAAGAAat gactgtgatc ataggcgctt
     3421 tgctgtttgc tctctatcgg caattgatta tcgagcagca gtatcaagtc actcaatatc
     3481 ttgagtcaga aactcagcgt tatcagcagc ttgcgcttac agtcgatcgc cgcagttttg
     3541 cggcacaaat tcgtgccgcc gatccgcaaa ccgcgcttat cgcttggcgc aatagttacg
     3601 atatggtcgg tgctttaagc tttatgcccg aaggcatgcc catgctgcca caaacgcggg
     3661 attttccgat attaaccggt gggccagata agctgcatat tcttaccggt ggtctggtaa
     3721 tgacccgcta tggccccgtc ttgattgcca ctcgtaccga taacttagct actcttatcg
     3781 ataaatttat cagcgcggca gccactgccg tgatgttgac tatcgtgctg accttggcgt
     3841 tgggctattt gttttctaag gcaattttac gccgtttagt gcaatacaac cgtttaagtg
     3901 agcaaattga gcgcggacat tatgacacgc gtttgccact cagttggcgt caggacgagt
     3961 tcgatatgtt agcgctgcaa tttaataacg tgctggatat tctcgaaaat aacctgatgg
     4021 cggtacgtgg tgtgacagat aatatcgccc acgatttacg cacaccctta tcccatatcc
     4081 ggattggtat tgaggagttg gcgaccaagt cccccgatga aattagcgaa ggttgtgcga
     4141 ttttgaccga agaactcgat cactgtcttg cgacttttga tgccatgctc tccctcacac
     4201 ggattgagga gggtcagcaa accttagatt tacaagagct gagtttggcc cagctctgca
     4261 cggatttgct ggaaatggcc gatgcggtag ctgagtcgaa tgagcaaacc ttgagtctgt
     4321 cgctacttgc tgatcataaa gttcatggcg ataaatattt gttattccaa gccttatata
     4381 atttggttga caatgctatg aagtactcag gccaaggcgc tcgtatcgat attattcagt
     4441 ccggcgctca aattaagatt tgcgataatg gcccagggat ccccgatgac agcaaagaaa
     4501 gagtgtttga gcgtttagtt cgcctcgacc caagccgcca tttgcaaggc acaggtttag
     4561 gattatctat ggtcaaagcc attctatcgc ggcacaatgc gcagattact ctttcggata
     4621 accatcctgg ccttgtggtc actatccagt tttaaTAATC TAGACCAGGC ATCAAATAAA
     4681 ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC
     4741 TCTCTACTAG AGTCACACTG GCTCACCTTC GGGTGGGCCT TTCTGCGTTT ATAGGTACCC
     4801 GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTT
//