LOCUS       pSR353                  4627 bp ds-DNA     circular     16-SEP-2016
DEFINITION  .
KEYWORDS    "Status:Template"                                                  
FEATURES             Location/Qualifiers
     CDS             942..1601
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2903..2920
                     /label="sRBS (4,296)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1633..1633)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1828..1833
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1730..1762
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3896..4522
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1809..1882
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1651..1651)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            1917..2567
                     /label="NarL (MG1655)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      4523..4627
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1851..1856
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3965..3988
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1681..1686)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1640..1645)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1559..1583
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2709..2813
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2986..3007
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    32..53
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2814..2825
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    4163..4185
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3653..3781
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3862..3895
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(133..815)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1664..1669)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2921..3640
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1658..1663)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             1883..1916
                     /label="sRBS 918.46"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2580..2708
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     terminator      820..925
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1863..1863
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3827..3861
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2858..2864
                     /label="narL heptamer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2858..2873
                     /label="narL 7-2-7 binding domain"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2866..2871
                     /label="-35 sigma"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2867..2873
                     /label="narL heptamer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2888..2893
                     /label="-10 sigma"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2826..2876
                     /label="narL-protected from DNaseI"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2901..2901
                     /label="sigma 70"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2826..2902
                     /label="PNarL3"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 CCTAGGCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
       61 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
      121 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      181 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      241 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      301 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG
      361 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      421 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      481 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      541 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
      601 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      661 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
      721 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
      781 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGACTAGT GCTTGGATTC TCACCAATAA
      841 AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA
      901 CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC
      961 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG
     1021 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
     1081 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
     1141 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
     1201 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
     1261 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
     1321 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
     1381 CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
     1441 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
     1501 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
     1561 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
     1621 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
     1681 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA
     1741 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC
     1801 ACCTCGAGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
     1861 ACATCAGCAG GACGCACTGA CCTAAGGATT TTTTCTAGAA GAATTATATA CAACAAatga
     1921 gtaatcagga accggctact atcctgctga ttgacgatca cccgatgctg cgaactggcg
     1981 taaaacagct tatcagtatg gcaccagata tcaccgtggt tggcgaagcg agtaatggcg
     2041 aacagggtat tgaactggcg gagtctcttg atcccgatct gatcctgtta gatctcaata
     2101 tgcccggcat gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctcagggc
     2161 gcattgtggt attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg
     2221 gcgcggatgg ctatctgtta aaagatatgg aaccggaaga tctgctgaaa gcattgcatc
     2281 aggcagctgc tggcgaaatg gtattaagcg aagcattaac gcctgttctg gccgccagct
     2341 tgcgcgctaa ccgtgccact actgagcgcg atgttaacca gttaacccca cgcgagcgcg
     2401 atattctcaa gctgattgcc cagggtttgc cgaacaagat gattgcccgc cgcctggata
     2461 tcaccgaaag cacagtaaaa gtgcacgtca agcacatgct gaagaaaatg aagctcaagt
     2521 ctcgcgtgga agcagcggta tgggtgcatc aggagcgcat tttctgaTAA TAATCTAGAC
     2581 CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG
     2641 TTTGTCGGTG AACGCTCTCT ACTAGAGTCA CACTGGCTCA CCTTCGGGTG GGCCTTTCTG
     2701 CGTTTATAGT AAATCACTGC ATAATTCGTG TCGCTCAAGG CGCACTCCCG TTCTGGATAA
     2761 TGTTTTTTGC GCCGACATCA TAACGGTTCT GGCAAATATT CTGAAATGAG CTGGTTAGCT
     2821 AGTCAAGAGT CTCTTCCCCT ATATAGCCAA TCATTCTTAC TCCCTTTGAA TTACCCGCCT
     2881 CATCAGAGAT AATGCTTAAG AAAAAGAGGA GAAATACTAG ATGCGTAAAG GCGAAGAGCT
     2941 GTTCACTGGT GTCGTCCCTA TTCTGGTGGA ACTGGATGGT GATGTCAACG GTCATAAGTT
     3001 TTCCGTGCGT GGCGAGGGTG AAGGTGACGC AACTAATGGT AAACTGACGC TGAAGTTCAT
     3061 CTGTACTACT GGTAAACTGC CGGTACCTTG GCCGACTCTG GTAACGACGC TGACTTATGG
     3121 TGTTCAGTGC TTTGCTCGTT ATCCGGACCA TATGAAGCAG CATGACTTCT TCAAGTCCGC
     3181 CATGCCGGAA GGCTATGTGC AGGAACGCAC GATTTCCTTT AAGGATGACG GCACGTACAA
     3241 AACGCGTGCG GAAGTGAAAT TTGAAGGCGA TACCCTGGTA AACCGCATTG AGCTGAAAGG
     3301 CATTGACTTT AAAGAAGACG GCAATATCCT GGGCCATAAG CTGGAATACA ATTTTAACAG
     3361 CCACAATGTT TACATCACCG CCGATAAACA AAAAAATGGC ATTAAAGCGA ATTTTAAAAT
     3421 TCGCCACAAC GTGGAGGATG GCAGCGTGCA GCTGGCTGAT CACTACCAGC AAAACACTCC
     3481 AATCGGTGAT GGTCCTGTTC TGCTGCCAGA CAATCACTAT CTGAGCACGC AAAGCGTTCT
     3541 GTCTAAAGAT CCGAACGAGA AACGCGATCA TATGGTTCTG CTGGAGTTCG TAACCGCAGC
     3601 GGGCATCACG CATGGTATGG ATGAACTGTA CAAATGATGA TAATAATCTA GACCAGGCAT
     3661 CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG
     3721 GTGAACGCTC TCTACTAGAG TCACACTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTTAT
     3781 AGGTACCCGT GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTTTTA CGGCTAGCTC
     3841 AGTCCTAGGT ATAGTGCTAG CCCAGCCAGA GAAACACTCT TTAACAGGGG GTAGTATGAT
     3901 GTCTCGTTTA GATAAAAGTA AAGTGATTAA CAGCGCATTA GAGCTGCTTA ATGAGGTCGG
     3961 AATCGAAGGT TTAACAACCC GTAAACTCGC CCAGAAGCTA GGTGTAGAGC AGCCTACATT
     4021 GTATTGGCAT GTAAAAAATA AGCGGGCTTT GCTCGACGCC TTAGCCATTG AGATGTTAGA
     4081 TAGGCACCAT ACTCACTTTT GCCCTTTAGA AGGGGAAAGC TGGCAAGATT TTTTACGTAA
     4141 TAACGCTAAA AGTTTTAGAT GTGCTTTACT AAGTCATCGC GATGGAGCAA AAGTACATTT
     4201 AGGTACACGG CCTACAGAAA AACAGTATGA AACTCTCGAA AATCAATTAG CCTTTTTATG
     4261 CCAACAAGGT TTTTCACTAG AGAATGCATT ATATGCACTC AGCGCAGTGG GGCATTTTAC
     4321 TTTAGGTTGC GTATTGGAAG ATCAAGAGCA TCAAGTCGCT AAAGAAGAAA GGGAAACACC
     4381 TACTACTGAT AGTATGCCGC CATTATTACG ACAAGCTATC GAATTATTTG ATCACCAAGG
     4441 TGCAGAGCCA GCCTTCTTAT TCGGCCTTGA ATTGATCATA TGCGGATTAG AAAAACAACT
     4501 TAAATGTGAA AGTGGGTCTT AAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG
     4561 GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG
     4621 CCCTAGA
//