LOCUS       pSR300.3                4590 bp ds-DNA     circular     27-SEP-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2810..2829
                     /label="UhpB binding site (inverted repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4126..4148
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2787..2806
                     /label="UhpB binding site (inverted repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2830..2835
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1559..1583
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1918..2508
                     /label="UhpA (NarL-FixJ; Eco)"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    2862..2862
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1651..1651)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2949..2970
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2849..2854
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2866..2883
                     /label="sRBS (813)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3790..3824
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3859..4485
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3825..3858
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      820..925
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1863..1863
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1828..1833
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1640..1645)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2755..2766
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    2650..2754
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    32..53
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3616..3744
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2521..2649
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2767..2865
                     /label="PuhpT99"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1809..1882
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      4486..4590
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    complement(1633..1633)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1681..1686)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1658..1663)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1664..1669)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1730..1762
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             942..1601
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(133..815)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2884..3603
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3928..3951
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1851..1856
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1883..1917
                     /label="sRBS (1,039)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 CCTAGGCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
       61 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
      121 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      181 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      241 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      301 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG
      361 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      421 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      481 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      541 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
      601 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      661 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
      721 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
      781 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGACTAGT GCTTGGATTC TCACCAATAA
      841 AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA
      901 CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC
      961 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG
     1021 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
     1081 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
     1141 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
     1201 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
     1261 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
     1321 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
     1381 CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
     1441 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
     1501 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
     1561 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
     1621 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
     1681 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA
     1741 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC
     1801 ACCTCGAGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
     1861 ACATCAGCAG GACGCACTGA CCAGTTCTAA GACGTAAAAA AGTAGATAGG ACGCCATATG
     1921 ATCACCGTTG CCCTTATAGA CGATCACCTC ATCGTCCGCT CCGGCTTTGC GCAGCTGCTG
     1981 GGGCTGGAAC CTGATTTGCA GGTAGTTGCC GAGTTTGGTT CGGGGCGCGA GGCGCTGGCG
     2041 GGGCTGCCGG GGCGCGGTGT GCAGGTGTGT ATTTGCGATA TCTCCATGCC CGATATCTCC
     2101 GGTCTGGAGC TGCTAAGCCA GCTGCCGAAA GGTATGGCGA CGATTATGCT CTCCGTTCAC
     2161 GACAGTCCTG CGCTGGTTGA GCAGGCGCTT AACGCGGGGG CACGCGGCTT TCTTTCCAAA
     2221 CGCTGTAGCC CGGATGAACT CATTGCTGCG GTGCATACGG TTGCCACGGG CGGCTGTTAT
     2281 CTGACGCCGG ATATTGCCAT TAAACTGGCA TCCGGTCGTC AGGACCCGCT AACCAAACGT
     2341 GAACGCCAGG TGGCGGAAAA ACTGGCGCAA GGAATGGCGG TGAAAGAGAT TGCCGCCGAA
     2401 CTGGGCTTGT CACCGAAAAC GGTACACGTC CATCGCGCCA ATCTGATGGA AAAACTGGGC
     2461 GTCAGTAACG ACGTAGAGCT GGCGCGCCGC ATGTTTGATG GCTGGTGATA ATAATCTAGA
     2521 CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT
     2581 GTTTGTCGGT GAACGCTCTC TACTAGAGTC ACACTGGCTC ACCTTCGGGT GGGCCTTTCT
     2641 GCGTTTATAG TAAATCACTG CATAATTCGT GTCGCTCAAG GCGCACTCCC GTTCTGGATA
     2701 ATGTTTTTTG CGCCGACATC ATAACGGTTC TGGCAAATAT TCTGAAATGA GCTGGTTAGC
     2761 TAGTCATTTC ACTTCATATC CGGCAAAACT AAGAAATTTT CCAGGTTTTG CCTGGACGCT
     2821 ATCTCAGGCC TGATTTGCTG CTGATTTTTA CAATGCATGC CTCACAAAGA GGAGAAATAC
     2881 TAGATGCGTA AAGGCGAAGA GCTGTTCACT GGTGTCGTCC CTATTCTGGT GGAACTGGAT
     2941 GGTGATGTCA ACGGTCATAA GTTTTCCGTG CGTGGCGAGG GTGAAGGTGA CGCAACTAAT
     3001 GGTAAACTGA CGCTGAAGTT CATCTGTACT ACTGGTAAAC TGCCGGTACC TTGGCCGACT
     3061 CTGGTAACGA CGCTGACTTA TGGTGTTCAG TGCTTTGCTC GTTATCCGGA CCATATGAAG
     3121 CAGCATGACT TCTTCAAGTC CGCCATGCCG GAAGGCTATG TGCAGGAACG CACGATTTCC
     3181 TTTAAGGATG ACGGCACGTA CAAAACGCGT GCGGAAGTGA AATTTGAAGG CGATACCCTG
     3241 GTAAACCGCA TTGAGCTGAA AGGCATTGAC TTTAAAGAAG ACGGCAATAT CCTGGGCCAT
     3301 AAGCTGGAAT ACAATTTTAA CAGCCACAAT GTTTACATCA CCGCCGATAA ACAAAAAAAT
     3361 GGCATTAAAG CGAATTTTAA AATTCGCCAC AACGTGGAGG ATGGCAGCGT GCAGCTGGCT
     3421 GATCACTACC AGCAAAACAC TCCAATCGGT GATGGTCCTG TTCTGCTGCC AGACAATCAC
     3481 TATCTGAGCA CGCAAAGCGT TCTGTCTAAA GATCCGAACG AGAAACGCGA TCATATGGTT
     3541 CTGCTGGAGT TCGTAACCGC AGCGGGCATC ACGCATGGTA TGGATGAACT GTACAAATGA
     3601 TGATAATAAT CTAGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT
     3661 TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT
     3721 CGGGTGGGCC TTTCTGCGTT TATAGGTACC CGTGGATCCT CTAGAGTCGA CCTGCAGGCA
     3781 TGCAAGCTTT TTACGGCTAG CTCAGTCCTA GGTATAGTGC TAGCCCAGCC AGAGAAACAC
     3841 TCTTTAACAG GGGGTAGTAT GATGTCTCGT TTAGATAAAA GTAAAGTGAT TAACAGCGCA
     3901 TTAGAGCTGC TTAATGAGGT CGGAATCGAA GGTTTAACAA CCCGTAAACT CGCCCAGAAG
     3961 CTAGGTGTAG AGCAGCCTAC ATTGTATTGG CATGTAAAAA ATAAGCGGGC TTTGCTCGAC
     4021 GCCTTAGCCA TTGAGATGTT AGATAGGCAC CATACTCACT TTTGCCCTTT AGAAGGGGAA
     4081 AGCTGGCAAG ATTTTTTACG TAATAACGCT AAAAGTTTTA GATGTGCTTT ACTAAGTCAT
     4141 CGCGATGGAG CAAAAGTACA TTTAGGTACA CGGCCTACAG AAAAACAGTA TGAAACTCTC
     4201 GAAAATCAAT TAGCCTTTTT ATGCCAACAA GGTTTTTCAC TAGAGAATGC ATTATATGCA
     4261 CTCAGCGCAG TGGGGCATTT TACTTTAGGT TGCGTATTGG AAGATCAAGA GCATCAAGTC
     4321 GCTAAAGAAG AAAGGGAAAC ACCTACTACT GATAGTATGC CGCCATTATT ACGACAAGCT
     4381 ATCGAATTAT TTGATCACCA AGGTGCAGAG CCAGCCTTCT TATTCGGCCT TGAATTGATC
     4441 ATATGCGGAT TAGAAAAACA ACTTAAATGT GAAAGTGGGT CTTAAGGCAT CAAATAAAAC
     4501 GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC
     4561 TCCTGAGTAG GACAAATCCG CCGCCCTAGA
//