LOCUS       pSR40-25                4789 bp ds-DNA     circular     07-APR-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2986..2997
                     /label="Stop region"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4163..4789
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2023..2352
                     /label="ompR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4094..4128
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2881..2985
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1988..2022
                     /label="sRBS (500)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1664..1688
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2752..2880
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1968..1987
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4232..4255
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3188..3907
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1914..1967
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4129..4162
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2353..2739
                     /label="ccaR"
                     /ApEinfo_revcolor=#09ac0e
                     /ApEinfo_fwdcolor=#09ac0e
     misc_feature    137..158
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4430..4452
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(2998..3169)
                     /label="cpcG2 upstream region short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2047..2065
                     /label="Sequencing primer rev SR132"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3253..3274
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3920..4048
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGACGTCTA AGAAACCATT ATTATCATGA
     1861 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGTCCCTAT
     1921 CAGTGATAGA GATTGACATC CCTATCAGTG ATAGAGATAC TGAGCACATC AGCAGGACGC
     1981 ACTGACCGCA TAAAGGACTT AGCAAAGCCC AATTTTAAAC AAATGCAAGA GAACTACAAG
     2041 ATTCTGGTGG TCGATGACGA CATGCGCCTG CGTGCGCTGC TGGAACGTTA TCTCACCGAA
     2101 CAAGGCTTCC AGGTTCGAAG CGTCGCTAAT GCAGAACAGA TGGATCGCCT GCTGACTCGT
     2161 GAATCTTTCC ATCTTATGGT ACTGGATTTA ATGTTACCTG GTGAAGATGG CTTGTCGATT
     2221 TGCCGACGTC TTCGTAGTCA GAGCAACCCG ATGCCGATCA TTATGGTGAC GGCGAAAGGG
     2281 GAAGAAGTGG ACCGTATCGT AGGCCTGGAG ATTGGCGCTG ACGACTACAT TCCAAAACCG
     2341 TTTAACCCGC GTGAGTTATT TGCCAGGGTG CGAGCTTTGT TGCGTCGGGG TTGTGCAACG
     2401 TGCCAACCAG TTTTAGAGTG GGGGCCAATC AGGTTGGATC CAAGCACCTA TGAAGTTAGT
     2461 TATGACAATG AGGTTTTGTC TTTGACCCGC AAGGAATACA GCATTCTGGA ATTACTACTC
     2521 CGCAATGGCC GTCGGGTGCT AAGTCGGAGC ATGATTATCG ATAGTATCTG GAAGTTGGAG
     2581 AGTCCCCCAG AGGAAGATAC GGTTAAGGTG CATGTGCGGA GTTTGCGACA AAAATTAAAA
     2641 AGTGCCGGTT TATCAGCAGA TGCCATTGAA ACGGTCCATG GCATTGGGTA TCGTCTGGCC
     2701 AATTTAACGG AAAAATCTTT GTGCCAAGGG AAAAACTAGT AATAATCTAG ACCAGGCATC
     2761 AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG
     2821 TGAACGCTCT CTACTAGAGT CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA
     2881 GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT AATGTTTTTT
     2941 GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG AGCTGGTTAG CTAGTCAAGC
     3001 CCATTGTGCT TTTCTCTATC AACCTCAGCT TACCTGAAGG GGTGAACAGG TCTGGGTTAA
     3061 TTCATGTTGC GAAATGTAAC AGTTTTAGTC GCATCAGCTA ACTTTCCGAT TTCTTTACGA
     3121 TTTTCTCCCC CTTTTCTTCA ATTTTACTTT GTTAGGATCG CATTTTTAAA AAGAGGAGAA
     3181 ATACTAGATG CGTAAAGGCG AAGAGCTGTT CACTGGTGTC GTCCCTATTC TGGTGGAACT
     3241 GGATGGTGAT GTCAACGGTC ATAAGTTTTC CGTGCGTGGC GAGGGTGAAG GTGACGCAAC
     3301 TAATGGTAAA CTGACGCTGA AGTTCATCTG TACTACTGGT AAACTGCCGG TACCTTGGCC
     3361 GACTCTGGTA ACGACGCTGA CTTATGGTGT TCAGTGCTTT GCTCGTTATC CGGACCATAT
     3421 GAAGCAGCAT GACTTCTTCA AGTCCGCCAT GCCGGAAGGC TATGTGCAGG AACGCACGAT
     3481 TTCCTTTAAG GATGACGGCA CGTACAAAAC GCGTGCGGAA GTGAAATTTG AAGGCGATAC
     3541 CCTGGTAAAC CGCATTGAGC TGAAAGGCAT TGACTTTAAA GAAGACGGCA ATATCCTGGG
     3601 CCATAAGCTG GAATACAATT TTAACAGCCA CAATGTTTAC ATCACCGCCG ATAAACAAAA
     3661 AAATGGCATT AAAGCGAATT TTAAAATTCG CCACAACGTG GAGGATGGCA GCGTGCAGCT
     3721 GGCTGATCAC TACCAGCAAA ACACTCCAAT CGGTGATGGT CCTGTTCTGC TGCCAGACAA
     3781 TCACTATCTG AGCACGCAAA GCGTTCTGTC TAAAGATCCG AACGAGAAAC GCGATCATAT
     3841 GGTTCTGCTG GAGTTCGTAA CCGCAGCGGG CATCACGCAT GGTATGGATG AACTGTACAA
     3901 ATGATGATAA TAATCTAGAC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG
     3961 GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCT ACTAGAGTCA CACTGGCTCA
     4021 CCTTCGGGTG GGCCTTTCTG CGTTTATAGG TACCCGTGGA TCCTCTAGAG TCGACCTGCA
     4081 GGCATGCAAG CTTTTTACGG CTAGCTCAGT CCTAGGTATA GTGCTAGCCC AGCCAGAGAA
     4141 ACACTCTTTA ACAGGGGGTA GTATGATGTC TCGTTTAGAT AAAAGTAAAG TGATTAACAG
     4201 CGCATTAGAG CTGCTTAATG AGGTCGGAAT CGAAGGTTTA ACAACCCGTA AACTCGCCCA
     4261 GAAGCTAGGT GTAGAGCAGC CTACATTGTA TTGGCATGTA AAAAATAAGC GGGCTTTGCT
     4321 CGACGCCTTA GCCATTGAGA TGTTAGATAG GCACCATACT CACTTTTGCC CTTTAGAAGG
     4381 GGAAAGCTGG CAAGATTTTT TACGTAATAA CGCTAAAAGT TTTAGATGTG CTTTACTAAG
     4441 TCATCGCGAT GGAGCAAAAG TACATTTAGG TACACGGCCT ACAGAAAAAC AGTATGAAAC
     4501 TCTCGAAAAT CAATTAGCCT TTTTATGCCA ACAAGGTTTT TCACTAGAGA ATGCATTATA
     4561 TGCACTCAGC GCAGTGGGGC ATTTTACTTT AGGTTGCGTA TTGGAAGATC AAGAGCATCA
     4621 AGTCGCTAAA GAAGAAAGGG AAACACCTAC TACTGATAGT ATGCCGCCAT TATTACGACA
     4681 AGCTATCGAA TTATTTGATC ACCAAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT
     4741 GATCATATGC GGATTAGAAA AACAACTTAA ATGTGAAAGT GGGTCTTAA
//