LOCUS       pSR40-28                4810 bp ds-DNA     circular     07-APR-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             4184..4810
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4115..4149
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3007..3018
                     /label="Stop region"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3274..3295
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2023..2436
                     /label="ompR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        1968..1987
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    3941..4069
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    4253..4276
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2902..3006
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1914..1967
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    137..158
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(3019..3190)
                     /label="cpcG2 upstream region short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4150..4183
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2773..2901
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             2437..2760
                     /label="ccaR"
                     /ApEinfo_revcolor=#09ac0e
                     /ApEinfo_fwdcolor=#09ac0e
     misc_feature    4451..4473
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3209..3928
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1988..2022
                     /label="sRBS (500)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1664..1688
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2047..2065
                     /label="Sequencing primer rev SR132"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGACGTCTA AGAAACCATT ATTATCATGA
     1861 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGTCCCTAT
     1921 CAGTGATAGA GATTGACATC CCTATCAGTG ATAGAGATAC TGAGCACATC AGCAGGACGC
     1981 ACTGACCGCA TAAAGGACTT AGCAAAGCCC AATTTTAAAC AAATGCAAGA GAACTACAAG
     2041 ATTCTGGTGG TCGATGACGA CATGCGCCTG CGTGCGCTGC TGGAACGTTA TCTCACCGAA
     2101 CAAGGCTTCC AGGTTCGAAG CGTCGCTAAT GCAGAACAGA TGGATCGCCT GCTGACTCGT
     2161 GAATCTTTCC ATCTTATGGT ACTGGATTTA ATGTTACCTG GTGAAGATGG CTTGTCGATT
     2221 TGCCGACGTC TTCGTAGTCA GAGCAACCCG ATGCCGATCA TTATGGTGAC GGCGAAAGGG
     2281 GAAGAAGTGG ACCGTATCGT AGGCCTGGAG ATTGGCGCTG ACGACTACAT TCCAAAACCG
     2341 TTTAACCCGC GTGAACTGCT GGCCCGTATC CGTGCGGTGC TGCGTCGTCA GGCGAACGAA
     2401 CTGCCAGGCG CACCGTCACA GGAAGAGGCG GTAATTGAGT GGGGGCCAAT CAGGTTGGAT
     2461 CCAAGCACCT ATGAAGTTAG TTATGACAAT GAGGTTTTGT CTTTGACCCG CAAGGAATAC
     2521 AGCATTCTGG AATTACTACT CCGCAATGGC CGTCGGGTGC TAAGTCGGAG CATGATTATC
     2581 GATAGTATCT GGAAGTTGGA GAGTCCCCCA GAGGAAGATA CGGTTAAGGT GCATGTGCGG
     2641 AGTTTGCGAC AAAAATTAAA AAGTGCCGGT TTATCAGCAG ATGCCATTGA AACGGTCCAT
     2701 GGCATTGGGT ATCGTCTGGC CAATTTAACG GAAAAATCTT TGTGCCAAGG GAAAAACTAG
     2761 TAATAATCTA GACCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC
     2821 GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCTACTAGAG TCACACTGGC TCACCTTCGG
     2881 GTGGGCCTTT CTGCGTTTAT AGTAAATCAC TGCATAATTC GTGTCGCTCA AGGCGCACTC
     2941 CCGTTCTGGA TAATGTTTTT TGCGCCGACA TCATAACGGT TCTGGCAAAT ATTCTGAAAT
     3001 GAGCTGGTTA GCTAGTCAAG CCCATTGTGC TTTTCTCTAT CAACCTCAGC TTACCTGAAG
     3061 GGGTGAACAG GTCTGGGTTA ATTCATGTTG CGAAATGTAA CAGTTTTAGT CGCATCAGCT
     3121 AACTTTCCGA TTTCTTTACG ATTTTCTCCC CCTTTTCTTC AATTTTACTT TGTTAGGATC
     3181 GCATTTTTAA AAAGAGGAGA AATACTAGAT GCGTAAAGGC GAAGAGCTGT TCACTGGTGT
     3241 CGTCCCTATT CTGGTGGAAC TGGATGGTGA TGTCAACGGT CATAAGTTTT CCGTGCGTGG
     3301 CGAGGGTGAA GGTGACGCAA CTAATGGTAA ACTGACGCTG AAGTTCATCT GTACTACTGG
     3361 TAAACTGCCG GTACCTTGGC CGACTCTGGT AACGACGCTG ACTTATGGTG TTCAGTGCTT
     3421 TGCTCGTTAT CCGGACCATA TGAAGCAGCA TGACTTCTTC AAGTCCGCCA TGCCGGAAGG
     3481 CTATGTGCAG GAACGCACGA TTTCCTTTAA GGATGACGGC ACGTACAAAA CGCGTGCGGA
     3541 AGTGAAATTT GAAGGCGATA CCCTGGTAAA CCGCATTGAG CTGAAAGGCA TTGACTTTAA
     3601 AGAAGACGGC AATATCCTGG GCCATAAGCT GGAATACAAT TTTAACAGCC ACAATGTTTA
     3661 CATCACCGCC GATAAACAAA AAAATGGCAT TAAAGCGAAT TTTAAAATTC GCCACAACGT
     3721 GGAGGATGGC AGCGTGCAGC TGGCTGATCA CTACCAGCAA AACACTCCAA TCGGTGATGG
     3781 TCCTGTTCTG CTGCCAGACA ATCACTATCT GAGCACGCAA AGCGTTCTGT CTAAAGATCC
     3841 GAACGAGAAA CGCGATCATA TGGTTCTGCT GGAGTTCGTA ACCGCAGCGG GCATCACGCA
     3901 TGGTATGGAT GAACTGTACA AATGATGATA ATAATCTAGA CCAGGCATCA AATAAAACGA
     3961 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC
     4021 TACTAGAGTC ACACTGGCTC ACCTTCGGGT GGGCCTTTCT GCGTTTATAG GTACCCGTGG
     4081 ATCCTCTAGA GTCGACCTGC AGGCATGCAA GCTTTTTACG GCTAGCTCAG TCCTAGGTAT
     4141 AGTGCTAGCC CAGCCAGAGA AACACTCTTT AACAGGGGGT AGTATGATGT CTCGTTTAGA
     4201 TAAAAGTAAA GTGATTAACA GCGCATTAGA GCTGCTTAAT GAGGTCGGAA TCGAAGGTTT
     4261 AACAACCCGT AAACTCGCCC AGAAGCTAGG TGTAGAGCAG CCTACATTGT ATTGGCATGT
     4321 AAAAAATAAG CGGGCTTTGC TCGACGCCTT AGCCATTGAG ATGTTAGATA GGCACCATAC
     4381 TCACTTTTGC CCTTTAGAAG GGGAAAGCTG GCAAGATTTT TTACGTAATA ACGCTAAAAG
     4441 TTTTAGATGT GCTTTACTAA GTCATCGCGA TGGAGCAAAA GTACATTTAG GTACACGGCC
     4501 TACAGAAAAA CAGTATGAAA CTCTCGAAAA TCAATTAGCC TTTTTATGCC AACAAGGTTT
     4561 TTCACTAGAG AATGCATTAT ATGCACTCAG CGCAGTGGGG CATTTTACTT TAGGTTGCGT
     4621 ATTGGAAGAT CAAGAGCATC AAGTCGCTAA AGAAGAAAGG GAAACACCTA CTACTGATAG
     4681 TATGCCGCCA TTATTACGAC AAGCTATCGA ATTATTTGAT CACCAAGGTG CAGAGCCAGC
     4741 CTTCTTATTC GGCCTTGAAT TGATCATATG CGGATTAGAA AAACAACTTA AATGTGAAAG
     4801 TGGGTCTTAA
//