LOCUS       pSR276.4                4736 bp ds-DNA     circular     05-OCT-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2284..2643
                     /label="PsdR DBD"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2996..2996
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1658..1663)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      820..925
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     CDS             4005..4631
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1851..1856
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1633..1633)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4272..4294
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3030..3749
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1730..1762
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1809..1882
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1828..1833
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(133..815)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2785..2889
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1559..1583
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             942..1601
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2902..3011
                     /label="PpsdA110"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3012..3029
                     /label="sRBS (4,296)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1863..1863
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3762..3890
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3971..4004
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2925..2932
                     /label="YvcP/PsdR binding site (inverted repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1651..1651)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1681..1686)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1918..2283
                     /label="ompR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    32..53
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4074..4097
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3936..3970
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1664..1669)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1640..1645)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2984..2989
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2957..2962
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2890..2901
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    2913..2920
                     /label="YvcP/PsdR binding site (inverted repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1942..1960
                     /label="Sequencing primer rev SR132"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3095..3116
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2656..2784
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1883..1917
                     /label="sRBS (500)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      4632..4736
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
ORIGIN
        1 CCTAGGCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
       61 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
      121 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      181 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      241 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      301 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG
      361 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      421 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      481 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      541 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
      601 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      661 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
      721 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
      781 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGACTAGT GCTTGGATTC TCACCAATAA
      841 AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA
      901 CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC
      961 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG
     1021 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
     1081 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
     1141 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
     1201 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
     1261 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
     1321 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
     1381 CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
     1441 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
     1501 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
     1561 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
     1621 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
     1681 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA
     1741 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC
     1801 ACCTCGAGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
     1861 ACATCAGCAG GACGCACTGA CCGCATAAAG GACTTAGCAA AGCCCAATTT TAAACAAATG
     1921 CAAGAGAACT ACAAGATTCT GGTGGTCGAT GACGACATGC GCCTGCGTGC GCTGCTGGAA
     1981 CGTTATCTCA CCGAACAAGG CTTCCAGGTT CGAAGCGTCG CTAATGCAGA ACAGATGGAT
     2041 CGCCTGCTGA CTCGTGAATC TTTCCATCTT ATGGTACTGG ATTTAATGTT ACCTGGTGAA
     2101 GATGGCTTGT CGATTTGCCG ACGTCTTCGT AGTCAGAGCA ACCCGATGCC GATCATTATG
     2161 GTGACGGCGA AAGGGGAAGA AGTGGACCGT ATCGTAGGCC TGGAGATTGG CGCTGACGAC
     2221 TACATTCCAA AACCGTTTAA CCCGCGTGAA CTGCTGGCCC GTATCCGTGC GGTGCTGCGT
     2281 CGTACATATG GTGAATATTC GCCCTCTTTG CCGCAGGAAT CTAGAATAGT GGAGCTTGGC
     2341 GGGCTGACGA TTTATCCTGA TCAGAATGAG GCCGAATGGA ACAGCGTCCG GATTTTGTTT
     2401 TCCCAAAAAG AATTCCAGCT TCTCTCGATT TTTGTGAGGG AGCATAAAAA AATTGTCTCT
     2461 CGTGATGAAC TATTGGAGGC GTTATGGGAT GACGTTGATT TTGTCGATGA CAATACGTTA
     2521 ACGGTCAATG TCAATCGGCT GCGGAGAAAA CTGGAGAATG CAGGACTGAC AGATTGCATT
     2581 TCCACAATCA GAGGACAAGG TTATCAATTT CAAGTCAATC GGAAGGATGA AGCGGAATGT
     2641 TAATAATAAT CTAGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT
     2701 TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT
     2761 CGGGTGGGCC TTTCTGCGTT TATAGTAAAT CACTGCATAA TTCGTGTCGC TCAAGGCGCA
     2821 CTCCCGTTCT GGATAATGTT TTTTGCGCCG ACATCATAAC GGTTCTGGCA AATATTCTGA
     2881 AATGAGCTGG TTAGCTAGTC ACACCCTCGT GAATGTGACA GCATTGTAAG ATTGGGGAGC
     2941 GGAATTGCAA GAAAGTTCGA TGGGAGGATG CTGACTTCCT TTTTATAATA AAGAAAAAGG
     3001 AGGAGCAGAA CAAAGAGGAG AAATACTAGA TGCGTAAAGG CGAAGAGCTG TTCACTGGTG
     3061 TCGTCCCTAT TCTGGTGGAA CTGGATGGTG ATGTCAACGG TCATAAGTTT TCCGTGCGTG
     3121 GCGAGGGTGA AGGTGACGCA ACTAATGGTA AACTGACGCT GAAGTTCATC TGTACTACTG
     3181 GTAAACTGCC GGTACCTTGG CCGACTCTGG TAACGACGCT GACTTATGGT GTTCAGTGCT
     3241 TTGCTCGTTA TCCGGACCAT ATGAAGCAGC ATGACTTCTT CAAGTCCGCC ATGCCGGAAG
     3301 GCTATGTGCA GGAACGCACG ATTTCCTTTA AGGATGACGG CACGTACAAA ACGCGTGCGG
     3361 AAGTGAAATT TGAAGGCGAT ACCCTGGTAA ACCGCATTGA GCTGAAAGGC ATTGACTTTA
     3421 AAGAAGACGG CAATATCCTG GGCCATAAGC TGGAATACAA TTTTAACAGC CACAATGTTT
     3481 ACATCACCGC CGATAAACAA AAAAATGGCA TTAAAGCGAA TTTTAAAATT CGCCACAACG
     3541 TGGAGGATGG CAGCGTGCAG CTGGCTGATC ACTACCAGCA AAACACTCCA ATCGGTGATG
     3601 GTCCTGTTCT GCTGCCAGAC AATCACTATC TGAGCACGCA AAGCGTTCTG TCTAAAGATC
     3661 CGAACGAGAA ACGCGATCAT ATGGTTCTGC TGGAGTTCGT AACCGCAGCG GGCATCACGC
     3721 ATGGTATGGA TGAACTGTAC AAATGATGAT AATAATCTAG ACCAGGCATC AAATAAAACG
     3781 AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT
     3841 CTACTAGAGT CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA GGTACCCGTG
     3901 GATCCTCTAG AGTCGACCTG CAGGCATGCA AGCTTTTTAC GGCTAGCTCA GTCCTAGGTA
     3961 TAGTGCTAGC CCAGCCAGAG AAACACTCTT TAACAGGGGG TAGTATGATG TCTCGTTTAG
     4021 ATAAAAGTAA AGTGATTAAC AGCGCATTAG AGCTGCTTAA TGAGGTCGGA ATCGAAGGTT
     4081 TAACAACCCG TAAACTCGCC CAGAAGCTAG GTGTAGAGCA GCCTACATTG TATTGGCATG
     4141 TAAAAAATAA GCGGGCTTTG CTCGACGCCT TAGCCATTGA GATGTTAGAT AGGCACCATA
     4201 CTCACTTTTG CCCTTTAGAA GGGGAAAGCT GGCAAGATTT TTTACGTAAT AACGCTAAAA
     4261 GTTTTAGATG TGCTTTACTA AGTCATCGCG ATGGAGCAAA AGTACATTTA GGTACACGGC
     4321 CTACAGAAAA ACAGTATGAA ACTCTCGAAA ATCAATTAGC CTTTTTATGC CAACAAGGTT
     4381 TTTCACTAGA GAATGCATTA TATGCACTCA GCGCAGTGGG GCATTTTACT TTAGGTTGCG
     4441 TATTGGAAGA TCAAGAGCAT CAAGTCGCTA AAGAAGAAAG GGAAACACCT ACTACTGATA
     4501 GTATGCCGCC ATTATTACGA CAAGCTATCG AATTATTTGA TCACCAAGGT GCAGAGCCAG
     4561 CCTTCTTATT CGGCCTTGAA TTGATCATAT GCGGATTAGA AAAACAACTT AAATGTGAAA
     4621 GTGGGTCTTA AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT
     4681 TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGA
//