LOCUS       pSR284                  4757 bp ds-DNA     circular     20-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3091..3121
                     /label="Direct repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2749..2877
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    complement(1738..1738)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2389..2442
                     /label="Linker"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2995..3137
                     /label="PmntCAB143"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1988..2022
                     /label="sRBS (500)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3086..3091
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4200..4223
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1786..1791)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2983..2994
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    4398..4420
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3065..3070
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3078..3083
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1933..1938
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2389..2736
                     /label="ManR DBD"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4131..4757
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4062..4096
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1756..1756)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2497..2724
                     /label="DNA-binding domain"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1914..1987
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1769..1774)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1956..1961
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2047..2065
                     /label="Sequencing primer rev SR132"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2878..2982
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3104..3109
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1664..1688
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3138..3155
                     /label="sRBS (4,296)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1968..1968
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1763..1768)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2023..2388
                     /label="ompR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3156..3875
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3098..3098
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1835..1867
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3888..4016
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3117..3117
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    137..158
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1745..1750)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    3221..3242
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4097..4130
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGACGTCTA AGAAACCATT ATTATCATGA
     1861 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGTCCCTAT
     1921 CAGTGATAGA GATTGACATC CCTATCAGTG ATAGAGATAC TGAGCACATC AGCAGGACGC
     1981 ACTGACCGCA TAAAGGACTT AGCAAAGCCC AATTTTAAAC AAATGCAAGA GAACTACAAG
     2041 ATTCTGGTGG TCGATGACGA CATGCGCCTG CGTGCGCTGC TGGAACGTTA TCTCACCGAA
     2101 CAAGGCTTCC AGGTTCGAAG CGTCGCTAAT GCAGAACAGA TGGATCGCCT GCTGACTCGT
     2161 GAATCTTTCC ATCTTATGGT ACTGGATTTA ATGTTACCTG GTGAAGATGG CTTGTCGATT
     2221 TGCCGACGTC TTCGTAGTCA GAGCAACCCG ATGCCGATCA TTATGGTGAC GGCGAAAGGG
     2281 GAAGAAGTGG ACCGTATCGT AGGCCTGGAG ATTGGCGCTG ACGACTACAT TCCAAAACCG
     2341 TTTAACCCGC GTGAACTGCT GGCCCGTATC CGTGCGGTGC TGCGTCGTCA AAGCCACGGA
     2401 GAAACCATCA CGGAAACCCT GGGTGCAGTC AAAAATAATT TGCTCAGCGT CAACAACGTT
     2461 AGCCTAGATG TGGCCAATCA GGTGGCCTAT TGCCAGGGTC AACGCATTGC CCTTTCGGAA
     2521 AAGGAAGTGG CGCTGTTAAC CCTATTCTTA CAAGCTCCCG GACAAATTCT CAGTCACGAA
     2581 GAGATCTATT CCCACCTTTG GCCCGGCGAA AGCCCCCCCA GTAGTAACGT GCTGGCGGCC
     2641 CTGGTGCGAT TACTGCGGCG CAAAATTGAA CAGCCCAATG CCCCCCGCCT GATTAATTCC
     2701 GTTTACGGCA AGGGGTACTG TTTTGAGGCC AATTAGTAAT AATCTAGACC AGGCATCAAA
     2761 TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
     2821 ACGCTCTCTA CTAGAGTCAC ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC GTTTATAGTA
     2881 AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT TCTGGATAAT GTTTTTTGCG
     2941 CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC TGGTTAGCTA GTCAGTAAAA
     3001 AGCAGAAAGT TTTAGTGGTC AGAAAAAGCC CACGAGCTTA CCAGTTGGTA AAAGTTAAGA
     3061 AATTTTAACT CCCCGTTTTC ACTGCAATTA TGAGAAGATA ATGAAAAATG AGATGATCAT
     3121 GAAAGACCAT TATCACTAAA GAGGAGAAAT ACTAGATGCG TAAAGGCGAA GAGCTGTTCA
     3181 CTGGTGTCGT CCCTATTCTG GTGGAACTGG ATGGTGATGT CAACGGTCAT AAGTTTTCCG
     3241 TGCGTGGCGA GGGTGAAGGT GACGCAACTA ATGGTAAACT GACGCTGAAG TTCATCTGTA
     3301 CTACTGGTAA ACTGCCGGTA CCTTGGCCGA CTCTGGTAAC GACGCTGACT TATGGTGTTC
     3361 AGTGCTTTGC TCGTTATCCG GACCATATGA AGCAGCATGA CTTCTTCAAG TCCGCCATGC
     3421 CGGAAGGCTA TGTGCAGGAA CGCACGATTT CCTTTAAGGA TGACGGCACG TACAAAACGC
     3481 GTGCGGAAGT GAAATTTGAA GGCGATACCC TGGTAAACCG CATTGAGCTG AAAGGCATTG
     3541 ACTTTAAAGA AGACGGCAAT ATCCTGGGCC ATAAGCTGGA ATACAATTTT AACAGCCACA
     3601 ATGTTTACAT CACCGCCGAT AAACAAAAAA ATGGCATTAA AGCGAATTTT AAAATTCGCC
     3661 ACAACGTGGA GGATGGCAGC GTGCAGCTGG CTGATCACTA CCAGCAAAAC ACTCCAATCG
     3721 GTGATGGTCC TGTTCTGCTG CCAGACAATC ACTATCTGAG CACGCAAAGC GTTCTGTCTA
     3781 AAGATCCGAA CGAGAAACGC GATCATATGG TTCTGCTGGA GTTCGTAACC GCAGCGGGCA
     3841 TCACGCATGG TATGGATGAA CTGTACAAAT GATGATAATA ATCTAGACCA GGCATCAAAT
     3901 AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA
     3961 CGCTCTCTAC TAGAGTCACA CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATAGGTA
     4021 CCCGTGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT TTTTACGGCT AGCTCAGTCC
     4081 TAGGTATAGT GCTAGCCCAG CCAGAGAAAC ACTCTTTAAC AGGGGGTAGT ATGATGTCTC
     4141 GTTTAGATAA AAGTAAAGTG ATTAACAGCG CATTAGAGCT GCTTAATGAG GTCGGAATCG
     4201 AAGGTTTAAC AACCCGTAAA CTCGCCCAGA AGCTAGGTGT AGAGCAGCCT ACATTGTATT
     4261 GGCATGTAAA AAATAAGCGG GCTTTGCTCG ACGCCTTAGC CATTGAGATG TTAGATAGGC
     4321 ACCATACTCA CTTTTGCCCT TTAGAAGGGG AAAGCTGGCA AGATTTTTTA CGTAATAACG
     4381 CTAAAAGTTT TAGATGTGCT TTACTAAGTC ATCGCGATGG AGCAAAAGTA CATTTAGGTA
     4441 CACGGCCTAC AGAAAAACAG TATGAAACTC TCGAAAATCA ATTAGCCTTT TTATGCCAAC
     4501 AAGGTTTTTC ACTAGAGAAT GCATTATATG CACTCAGCGC AGTGGGGCAT TTTACTTTAG
     4561 GTTGCGTATT GGAAGATCAA GAGCATCAAG TCGCTAAAGA AGAAAGGGAA ACACCTACTA
     4621 CTGATAGTAT GCCGCCATTA TTACGACAAG CTATCGAATT ATTTGATCAC CAAGGTGCAG
     4681 AGCCAGCCTT CTTATTCGGC CTTGAATTGA TCATATGCGG ATTAGAAAAA CAACTTAAAT
     4741 GTGAAAGTGG GTCTTAA
//