LOCUS       pKD263                  4801 bp ds-DNA     circular     30-JUN-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    4141..4174
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1914..1967
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1988..2022
                     /label="sRBS (683.79)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2893..2997
                     /label="Ptrp Upstream Fragment"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1968..1987
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    3200..3919
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3932..4060
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             4175..4801
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3182..3199
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2158..2160
                     /label="P in Nissle"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4106..4140
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2425..2751
                     /label="ccaR"
                     /ApEinfo_revcolor=#09ac0e
                     /ApEinfo_fwdcolor=#09ac0e
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2023..2424
                     /label="TorR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2764..2843
                     /label="B0010"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2844..2851
                     /label="BB Scar"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      2764..2892
                     /label="B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2852..2892
                     /label="B0012"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3140..3140
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3128..3133
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3165..3170
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3114..3135
                     /label="G box"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3177..3177
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3010..3181
                     /label="cpcG2 Upstream Region"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3144..3149
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3101..3106
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3173..3173
                     /label="Transcription start site Syn (Kopf et al., 2014)"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGACGTCTA AGAAACCATT ATTATCATGA
     1861 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGTCCCTAT
     1921 CAGTGATAGA GATTGACATC CCTATCAGTG ATAGAGATAC TGAGCACATC AGCAGGACGC
     1981 ACTGACCTAA GCGTAAAATA AGTAAATTAC ACCTATACAA GTATGCCACA TCACATTGTT
     2041 ATTGTTGAAG ATGAGCCGGT TACCCAGGCG CGATTACAAT CCTACTTCAC TCAGGAGGGG
     2101 TATACCGTTT CCGTTACGGC GAGCGGTGCT GGGCTACGGG AAATTATGCA GAATCAGTCG
     2161 GTAGATTTAA TTCTGCTGGA TATCAACTTA CCCGATGAAA ATGGCCTGAT GTTAACCCGC
     2221 GCCCTGCGAG AACGCTCAAC GGTGGGGATT ATTCTGGTTA CCGGACGCAG CGATCGGATT
     2281 GACCGTATTG TTGGGCTGGA AATGGGCGCA GACGATTACG TCACCAAACC GCTGGAACTG
     2341 CGCGAACTGG TAGTACGGGT GAAAAATCTG CTCTGGCGAA TCGACCTCGC GCGACAAGCT
     2401 CAACCGCACA CTCAGGACAA CTGCTTAGAG TGGGGGCCAA TCAGGTTGGA TCCAAGCACC
     2461 TATGAAGTTA GTTATGACAA TGAGGTTTTG TCTTTGACCC GCAAGGAATA CAGCATTCTG
     2521 GAATTACTAC TCCGCAATGG CCGTCGGGTG CTAAGTCGGA GCATGATTAT CGATAGTATC
     2581 TGGAAGTTGG AGAGTCCCCC AGAGGAAGAT ACGGTTAAGG TGCATGTGCG GAGTTTGCGA
     2641 CAAAAATTAA AAAGTGCCGG TTTATCAGCA GATGCCATTG AAACGGTCCA TGGCATTGGG
     2701 TATCGTCTGG CCAATTTAAC GGAAAAATCT TTGTGCCAAG GGAAAAACTA GTAATAATCT
     2761 AGACCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT
     2821 GTTGTTTGTC GGTGAACGCT CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT
     2881 TCTGCGTTTA TAGTAAATCA CTGCATAATT CGTGTCGCTC AAGGCGCACT CCCGTTCTGG
     2941 ATAATGTTTT TTGCGCCGAC ATCATAACGG TTCTGGCAAA TATTCTGAAA TGAGCTGGTT
     3001 AGCTAGTCAA GCCCATTGTG CTTTTCTCTA TCAACCTCAG CTTACCTGAA GGGGTGAACA
     3061 GGTCTGGGTT AATTCATGTT GCGAAATGTA ACAGTTTTAG TCGCATCAGC TAACTTTCCG
     3121 ATTTCTTTAC GATTTTCTCC CCCTTTTCTT CAATTTTACT TTGTTAGGAT CGCATTTTTA
     3181 AAAAGAGGAG AAATACTAGA TGCGTAAAGG CGAAGAGCTG TTCACTGGTG TCGTCCCTAT
     3241 TCTGGTGGAA CTGGATGGTG ATGTCAACGG TCATAAGTTT TCCGTGCGTG GCGAGGGTGA
     3301 AGGTGACGCA ACTAATGGTA AACTGACGCT GAAGTTCATC TGTACTACTG GTAAACTGCC
     3361 GGTACCTTGG CCGACTCTGG TAACGACGCT GACTTATGGT GTTCAGTGCT TTGCTCGTTA
     3421 TCCGGACCAT ATGAAGCAGC ATGACTTCTT CAAGTCCGCC ATGCCGGAAG GCTATGTGCA
     3481 GGAACGCACG ATTTCCTTTA AGGATGACGG CACGTACAAA ACGCGTGCGG AAGTGAAATT
     3541 TGAAGGCGAT ACCCTGGTAA ACCGCATTGA GCTGAAAGGC ATTGACTTTA AAGAAGACGG
     3601 CAATATCCTG GGCCATAAGC TGGAATACAA TTTTAACAGC CACAATGTTT ACATCACCGC
     3661 CGATAAACAA AAAAATGGCA TTAAAGCGAA TTTTAAAATT CGCCACAACG TGGAGGATGG
     3721 CAGCGTGCAG CTGGCTGATC ACTACCAGCA AAACACTCCA ATCGGTGATG GTCCTGTTCT
     3781 GCTGCCAGAC AATCACTATC TGAGCACGCA AAGCGTTCTG TCTAAAGATC CGAACGAGAA
     3841 ACGCGATCAT ATGGTTCTGC TGGAGTTCGT AACCGCAGCG GGCATCACGC ATGGTATGGA
     3901 TGAACTGTAC AAATGATGAT AATAATCTAG ACCAGGCATC AAATAAAACG AAAGGCTCAG
     3961 TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CTACTAGAGT
     4021 CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA GGTACCCGTG GATCCTCTAG
     4081 AGTCGACCTG CAGGCATGCA AGCTTTTTAC GGCTAGCTCA GTCCTAGGTA TAGTGCTAGC
     4141 CCAGCCAGAG AAACACTCTT TAACAGGGGG TAGTATGATG TCTCGTTTAG ATAAAAGTAA
     4201 AGTGATTAAC AGCGCATTAG AGCTGCTTAA TGAGGTCGGA ATCGAAGGTT TAACAACCCG
     4261 TAAACTCGCC CAGAAGCTAG GTGTAGAGCA GCCTACATTG TATTGGCATG TAAAAAATAA
     4321 GCGGGCTTTG CTCGACGCCT TAGCCATTGA GATGTTAGAT AGGCACCATA CTCACTTTTG
     4381 CCCTTTAGAA GGGGAAAGCT GGCAAGATTT TTTACGTAAT AACGCTAAAA GTTTTAGATG
     4441 TGCTTTACTA AGTCATCGCG ATGGAGCAAA AGTACATTTA GGTACACGGC CTACAGAAAA
     4501 ACAGTATGAA ACTCTCGAAA ATCAATTAGC CTTTTTATGC CAACAAGGTT TTTCACTAGA
     4561 GAATGCATTA TATGCACTCA GCGCAGTGGG GCATTTTACT TTAGGTTGCG TATTGGAAGA
     4621 TCAAGAGCAT CAAGTCGCTA AAGAAGAAAG GGAAACACCT ACTACTGATA GTATGCCGCC
     4681 ATTATTACGA CAAGCTATCG AATTATTTGA TCACCAAGGT GCAGAGCCAG CCTTCTTATT
     4741 CGGCCTTGAA TTGATCATAT GCGGATTAGA AAAACAACTT AAATGTGAAA GTGGGTCTTA
     4801 A
//