LOCUS       pKD265                  4727 bp ds-DNA     circular     05-OCT-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    4067..4100
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3858..3986
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3053..3058
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3092..3092
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1968..1987
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     CDS             4101..4727
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2425..2739
                     /label="yvcP/psdR"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2881..2985
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3009..3016
                     /label="YvcP/PsdR binding site (inverted repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2158..2160
                     /label="P in Nissle"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3021..3028
                     /label="YvcP/PsdR binding site (inverted repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2023..2424
                     /label="TorR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3108..3125
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4032..4066
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2998..3107
                     /label="PpsdA110"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2986..2997
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    2752..2880
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1988..2022
                     /label="sRBS (683.79)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3126..3845
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1914..1967
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3080..3085
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGACGTCTA AGAAACCATT ATTATCATGA
     1861 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGTCCCTAT
     1921 CAGTGATAGA GATTGACATC CCTATCAGTG ATAGAGATAC TGAGCACATC AGCAGGACGC
     1981 ACTGACCTAA GCGTAAAATA AGTAAATTAC ACCTATACAA GTATGCCACA TCACATTGTT
     2041 ATTGTTGAAG ATGAGCCGGT TACCCAGGCG CGATTACAAT CCTACTTCAC TCAGGAGGGG
     2101 TATACCGTTT CCGTTACGGC GAGCGGTGCT GGGCTACGGG AAATTATGCA GAATCAGTCG
     2161 GTAGATTTAA TTCTGCTGGA TATCAACTTA CCCGATGAAA ATGGCCTGAT GTTAACCCGC
     2221 GCCCTGCGAG AACGCTCAAC GGTGGGGATT ATTCTGGTTA CCGGACGCAG CGATCGGATT
     2281 GACCGTATTG TTGGGCTGGA AATGGGCGCA GACGATTACG TCACCAAACC GCTGGAACTG
     2341 CGCGAACTGG TAGTACGGGT GAAAAATCTG CTCTGGCGAA TCGACCTCGC GCGACAAGCT
     2401 CAACCGCACA CTCAGGACAA CTGCGTGGAG CTTGGCGGGC TGACGATTTA TCCTGATCAG
     2461 AATGAGGCCG AATGGAACAG CGTCCGGATT TTGTTTTCCC AAAAAGAATT CCAGCTTCTC
     2521 TCGATTTTTG TGAGGGAGCA TAAAAAAATT GTCTCTCGTG ATGAACTATT GGAGGCGTTA
     2581 TGGGATGACG TTGATTTTGT CGATGACAAT ACGTTAACGG TCAATGTCAA TCGGCTGCGG
     2641 AGAAAACTGG AGAATGCAGG ACTGACAGAT TGCATTTCCA CAATCAGAGG ACAAGGTTAT
     2701 CAATTTCAAG TCAATCGGAA GGATGAAGCG GAATGTTAAT AATAATCTAG ACCAGGCATC
     2761 AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG
     2821 TGAACGCTCT CTACTAGAGT CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA
     2881 GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT AATGTTTTTT
     2941 GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG AGCTGGTTAG CTAGTCACAC
     3001 CCTCGTGAAT GTGACAGCAT TGTAAGATTG GGGAGCGGAA TTGCAAGAAA GTTCGATGGG
     3061 AGGATGCTGA CTTCCTTTTT ATAATAAAGA AAAAGGAGGA GCAGAACAAA GAGGAGAAAT
     3121 ACTAGATGCG TAAAGGCGAA GAGCTGTTCA CTGGTGTCGT CCCTATTCTG GTGGAACTGG
     3181 ATGGTGATGT CAACGGTCAT AAGTTTTCCG TGCGTGGCGA GGGTGAAGGT GACGCAACTA
     3241 ATGGTAAACT GACGCTGAAG TTCATCTGTA CTACTGGTAA ACTGCCGGTA CCTTGGCCGA
     3301 CTCTGGTAAC GACGCTGACT TATGGTGTTC AGTGCTTTGC TCGTTATCCG GACCATATGA
     3361 AGCAGCATGA CTTCTTCAAG TCCGCCATGC CGGAAGGCTA TGTGCAGGAA CGCACGATTT
     3421 CCTTTAAGGA TGACGGCACG TACAAAACGC GTGCGGAAGT GAAATTTGAA GGCGATACCC
     3481 TGGTAAACCG CATTGAGCTG AAAGGCATTG ACTTTAAAGA AGACGGCAAT ATCCTGGGCC
     3541 ATAAGCTGGA ATACAATTTT AACAGCCACA ATGTTTACAT CACCGCCGAT AAACAAAAAA
     3601 ATGGCATTAA AGCGAATTTT AAAATTCGCC ACAACGTGGA GGATGGCAGC GTGCAGCTGG
     3661 CTGATCACTA CCAGCAAAAC ACTCCAATCG GTGATGGTCC TGTTCTGCTG CCAGACAATC
     3721 ACTATCTGAG CACGCAAAGC GTTCTGTCTA AAGATCCGAA CGAGAAACGC GATCATATGG
     3781 TTCTGCTGGA GTTCGTAACC GCAGCGGGCA TCACGCATGG TATGGATGAA CTGTACAAAT
     3841 GATGATAATA ATCTAGACCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC
     3901 CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA CTGGCTCACC
     3961 TTCGGGTGGG CCTTTCTGCG TTTATAGGTA CCCGTGGATC CTCTAGAGTC GACCTGCAGG
     4021 CATGCAAGCT TTTTACGGCT AGCTCAGTCC TAGGTATAGT GCTAGCCCAG CCAGAGAAAC
     4081 ACTCTTTAAC AGGGGGTAGT ATGATGTCTC GTTTAGATAA AAGTAAAGTG ATTAACAGCG
     4141 CATTAGAGCT GCTTAATGAG GTCGGAATCG AAGGTTTAAC AACCCGTAAA CTCGCCCAGA
     4201 AGCTAGGTGT AGAGCAGCCT ACATTGTATT GGCATGTAAA AAATAAGCGG GCTTTGCTCG
     4261 ACGCCTTAGC CATTGAGATG TTAGATAGGC ACCATACTCA CTTTTGCCCT TTAGAAGGGG
     4321 AAAGCTGGCA AGATTTTTTA CGTAATAACG CTAAAAGTTT TAGATGTGCT TTACTAAGTC
     4381 ATCGCGATGG AGCAAAAGTA CATTTAGGTA CACGGCCTAC AGAAAAACAG TATGAAACTC
     4441 TCGAAAATCA ATTAGCCTTT TTATGCCAAC AAGGTTTTTC ACTAGAGAAT GCATTATATG
     4501 CACTCAGCGC AGTGGGGCAT TTTACTTTAG GTTGCGTATT GGAAGATCAA GAGCATCAAG
     4561 TCGCTAAAGA AGAAAGGGAA ACACCTACTA CTGATAGTAT GCCGCCATTA TTACGACAAG
     4621 CTATCGAATT ATTTGATCAC CAAGGTGCAG AGCCAGCCTT CTTATTCGGC CTTGAATTGA
     4681 TCATATGCGG ATTAGAAAAA CAACTTAAAT GTGAAAGTGG GTCTTAA
//