LOCUS       pKD281                  4706 bp ds-DNA     circular     02-AUG-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2977..3086
                     /label="PpsdA110"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4011..4045
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3000..3007
                     /label="YvcP/PsdR binding site (inverted repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1968..1987
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    1914..1967
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     CDS             4080..4706
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3059..3064
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3105..3824
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2965..2976
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    2731..2859
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    3087..3104
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3032..3037
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2173..2175
                     /label="D51"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2860..2964
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4046..4079
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2023..2403
                     /label="SO_4428"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3071..3071
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2988..2995
                     /label="YvcP/PsdR binding site (inverted repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2404..2718
                     /label="yvcP/psdR"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
     misc_feature    1988..2022
                     /label="sRBS (1,000)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3837..3965
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGACGTCTA AGAAACCATT ATTATCATGA
     1861 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGTCCCTAT
     1921 CAGTGATAGA GATTGACATC CCTATCAGTG ATAGAGATAC TGAGCACATC AGCAGGACGC
     1981 ACTGACCTAC CCAGCCCAGT AAGTACATAT TAGAAAGGGT TAatgaaaat acttttagtt
     2041 gaagatgatg cgacaacgat tgattacatc gttaaaggat ttcttgagca gggacataat
     2101 attgaaaccg ccagtgatgg tcaccaagga ctactgcaag ccacgagtgg ccagtatgat
     2161 ctgttgatcc tcgaccgcat gttgcctcaa ctcgatggtt taaaactctt ggccgccctc
     2221 agggctactg gcaatcaaac cccagtattg atcctgtcgg cgcttgccca tgtggatgaa
     2281 cgggtaaaag gtctgcgcgc aggcggcgat gattatatga caaagccctt tgctttttcg
     2341 gagctgttag tgcgtgccga aaagctgatg caacggggcc aatccacgcc cattaccaca
     2401 gatGTGGAGC TTGGCGGGCT GACGATTTAT CCTGATCAGA ATGAGGCCGA ATGGAACAGC
     2461 GTCCGGATTT TGTTTTCCCA AAAAGAATTC CAGCTTCTCT CGATTTTTGT GAGGGAGCAT
     2521 AAAAAAATTG TCTCTCGTGA TGAACTATTG GAGGCGTTAT GGGATGACGT TGATTTTGTC
     2581 GATGACAATA CGTTAACGGT CAATGTCAAT CGGCTGCGGA GAAAACTGGA GAATGCAGGA
     2641 CTGACAGATT GCATTTCCAC AATCAGAGGA CAAGGTTATC AATTTCAAGT CAATCGGAAG
     2701 GATGAAGCGG AATGTTAATA ATAATCTAGA CCAGGCATCA AATAAAACGA AAGGCTCAGT
     2761 CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC TACTAGAGTC
     2821 ACACTGGCTC ACCTTCGGGT GGGCCTTTCT GCGTTTATAG TAAATCACTG CATAATTCGT
     2881 GTCGCTCAAG GCGCACTCCC GTTCTGGATA ATGTTTTTTG CGCCGACATC ATAACGGTTC
     2941 TGGCAAATAT TCTGAAATGA GCTGGTTAGC TAGTCACACC CTCGTGAATG TGACAGCATT
     3001 GTAAGATTGG GGAGCGGAAT TGCAAGAAAG TTCGATGGGA GGATGCTGAC TTCCTTTTTA
     3061 TAATAAAGAA AAAGGAGGAG CAGAACAAAG AGGAGAAATA CTAGATGCGT AAAGGCGAAG
     3121 AGCTGTTCAC TGGTGTCGTC CCTATTCTGG TGGAACTGGA TGGTGATGTC AACGGTCATA
     3181 AGTTTTCCGT GCGTGGCGAG GGTGAAGGTG ACGCAACTAA TGGTAAACTG ACGCTGAAGT
     3241 TCATCTGTAC TACTGGTAAA CTGCCGGTAC CTTGGCCGAC TCTGGTAACG ACGCTGACTT
     3301 ATGGTGTTCA GTGCTTTGCT CGTTATCCGG ACCATATGAA GCAGCATGAC TTCTTCAAGT
     3361 CCGCCATGCC GGAAGGCTAT GTGCAGGAAC GCACGATTTC CTTTAAGGAT GACGGCACGT
     3421 ACAAAACGCG TGCGGAAGTG AAATTTGAAG GCGATACCCT GGTAAACCGC ATTGAGCTGA
     3481 AAGGCATTGA CTTTAAAGAA GACGGCAATA TCCTGGGCCA TAAGCTGGAA TACAATTTTA
     3541 ACAGCCACAA TGTTTACATC ACCGCCGATA AACAAAAAAA TGGCATTAAA GCGAATTTTA
     3601 AAATTCGCCA CAACGTGGAG GATGGCAGCG TGCAGCTGGC TGATCACTAC CAGCAAAACA
     3661 CTCCAATCGG TGATGGTCCT GTTCTGCTGC CAGACAATCA CTATCTGAGC ACGCAAAGCG
     3721 TTCTGTCTAA AGATCCGAAC GAGAAACGCG ATCATATGGT TCTGCTGGAG TTCGTAACCG
     3781 CAGCGGGCAT CACGCATGGT ATGGATGAAC TGTACAAATG ATGATAATAA TCTAGACCAG
     3841 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT
     3901 GTCGGTGAAC GCTCTCTACT AGAGTCACAC TGGCTCACCT TCGGGTGGGC CTTTCTGCGT
     3961 TTATAGGTAC CCGTGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT TTTACGGCTA
     4021 GCTCAGTCCT AGGTATAGTG CTAGCCCAGC CAGAGAAACA CTCTTTAACA GGGGGTAGTA
     4081 TGATGTCTCG TTTAGATAAA AGTAAAGTGA TTAACAGCGC ATTAGAGCTG CTTAATGAGG
     4141 TCGGAATCGA AGGTTTAACA ACCCGTAAAC TCGCCCAGAA GCTAGGTGTA GAGCAGCCTA
     4201 CATTGTATTG GCATGTAAAA AATAAGCGGG CTTTGCTCGA CGCCTTAGCC ATTGAGATGT
     4261 TAGATAGGCA CCATACTCAC TTTTGCCCTT TAGAAGGGGA AAGCTGGCAA GATTTTTTAC
     4321 GTAATAACGC TAAAAGTTTT AGATGTGCTT TACTAAGTCA TCGCGATGGA GCAAAAGTAC
     4381 ATTTAGGTAC ACGGCCTACA GAAAAACAGT ATGAAACTCT CGAAAATCAA TTAGCCTTTT
     4441 TATGCCAACA AGGTTTTTCA CTAGAGAATG CATTATATGC ACTCAGCGCA GTGGGGCATT
     4501 TTACTTTAGG TTGCGTATTG GAAGATCAAG AGCATCAAGT CGCTAAAGAA GAAAGGGAAA
     4561 CACCTACTAC TGATAGTATG CCGCCATTAT TACGACAAGC TATCGAATTA TTTGATCACC
     4621 AAGGTGCAGA GCCAGCCTTC TTATTCGGCC TTGAATTGAT CATATGCGGA TTAGAAAAAC
     4681 AACTTAAATG TGAAAGTGGG TCTTAA
//