LOCUS       pKD285                  4694 bp ds-DNA     circular     15-FEB-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1968..1987
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2953..2964
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    2719..2847
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3075..3092
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2170..2172
                     /label="D51"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3047..3052
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3825..3953
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2965..3074
                     /label="PpsdA110"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1988..2019
                     /label="sRBS (1,000)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2392..2706
                     /label="yvcP/psdR"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
     CDS             4068..4694
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3999..4033
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2988..2995
                     /label="YvcP/PsdR binding site (inverted repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2976..2983
                     /label="YvcP/PsdR binding site (inverted repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2848..2952
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3020..3025
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2020..2391
                     /label="SO_4623"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3093..3812
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3059..3059
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    4034..4067
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1914..1967
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGACGTCTA AGAAACCATT ATTATCATGA
     1861 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGTCCCTAT
     1921 CAGTGATAGA GATTGACATC CCTATCAGTG ATAGAGATAC TGAGCACATC AGCAGGACGC
     1981 ACTGACCCTT AAATCGGCCC CTCATAAGTC CCTTTTCACa tgttggtatt actggttgaa
     2041 gacaatcggc ttctctctaa caacatcatt caatatcttg aattgagtgg catagagtgc
     2101 gattacgcgt tcaacctagc gcaagcagaa atgttgatca gccagcagca atttgacgcc
     2161 atcatccttg atcttaattt accggatggt gatggcattg aggcgtgtga acgctggaaa
     2221 gcgcaatgtt ttagttcacc tatcatcatg ctaaccgccc gtagcagttt gaatgagcgc
     2281 ttggctggtt ttgctgttgg cgctgatgat tatttaataa aaccctttgc gatggaggag
     2341 ctggtcgcgc ggctgaaggt ggttgcccag cgtcgtcctg cgccccaaag gGTGGAGCTT
     2401 GGCGGGCTGA CGATTTATCC TGATCAGAAT GAGGCCGAAT GGAACAGCGT CCGGATTTTG
     2461 TTTTCCCAAA AAGAATTCCA GCTTCTCTCG ATTTTTGTGA GGGAGCATAA AAAAATTGTC
     2521 TCTCGTGATG AACTATTGGA GGCGTTATGG GATGACGTTG ATTTTGTCGA TGACAATACG
     2581 TTAACGGTCA ATGTCAATCG GCTGCGGAGA AAACTGGAGA ATGCAGGACT GACAGATTGC
     2641 ATTTCCACAA TCAGAGGACA AGGTTATCAA TTTCAAGTCA ATCGGAAGGA TGAAGCGGAA
     2701 TGTTAATAAT AATCTAGACC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG
     2761 CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCTA CTAGAGTCAC ACTGGCTCAC
     2821 CTTCGGGTGG GCCTTTCTGC GTTTATAGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC
     2881 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC
     2941 TGAAATGAGC TGGTTAGCTA GTCACACCCT CGTGAATGTG ACAGCATTGT AAGATTGGGG
     3001 AGCGGAATTG CAAGAAAGTT CGATGGGAGG ATGCTGACTT CCTTTTTATA ATAAAGAAAA
     3061 AGGAGGAGCA GAACAAAGAG GAGAAATACT AGATGCGTAA AGGCGAAGAG CTGTTCACTG
     3121 GTGTCGTCCC TATTCTGGTG GAACTGGATG GTGATGTCAA CGGTCATAAG TTTTCCGTGC
     3181 GTGGCGAGGG TGAAGGTGAC GCAACTAATG GTAAACTGAC GCTGAAGTTC ATCTGTACTA
     3241 CTGGTAAACT GCCGGTACCT TGGCCGACTC TGGTAACGAC GCTGACTTAT GGTGTTCAGT
     3301 GCTTTGCTCG TTATCCGGAC CATATGAAGC AGCATGACTT CTTCAAGTCC GCCATGCCGG
     3361 AAGGCTATGT GCAGGAACGC ACGATTTCCT TTAAGGATGA CGGCACGTAC AAAACGCGTG
     3421 CGGAAGTGAA ATTTGAAGGC GATACCCTGG TAAACCGCAT TGAGCTGAAA GGCATTGACT
     3481 TTAAAGAAGA CGGCAATATC CTGGGCCATA AGCTGGAATA CAATTTTAAC AGCCACAATG
     3541 TTTACATCAC CGCCGATAAA CAAAAAAATG GCATTAAAGC GAATTTTAAA ATTCGCCACA
     3601 ACGTGGAGGA TGGCAGCGTG CAGCTGGCTG ATCACTACCA GCAAAACACT CCAATCGGTG
     3661 ATGGTCCTGT TCTGCTGCCA GACAATCACT ATCTGAGCAC GCAAAGCGTT CTGTCTAAAG
     3721 ATCCGAACGA GAAACGCGAT CATATGGTTC TGCTGGAGTT CGTAACCGCA GCGGGCATCA
     3781 CGCATGGTAT GGATGAACTG TACAAATGAT GATAATAATC TAGACCAGGC ATCAAATAAA
     3841 ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC
     3901 TCTCTACTAG AGTCACACTG GCTCACCTTC GGGTGGGCCT TTCTGCGTTT ATAGGTACCC
     3961 GTGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTTT TACGGCTAGC TCAGTCCTAG
     4021 GTATAGTGCT AGCCCAGCCA GAGAAACACT CTTTAACAGG GGGTAGTATG ATGTCTCGTT
     4081 TAGATAAAAG TAAAGTGATT AACAGCGCAT TAGAGCTGCT TAATGAGGTC GGAATCGAAG
     4141 GTTTAACAAC CCGTAAACTC GCCCAGAAGC TAGGTGTAGA GCAGCCTACA TTGTATTGGC
     4201 ATGTAAAAAA TAAGCGGGCT TTGCTCGACG CCTTAGCCAT TGAGATGTTA GATAGGCACC
     4261 ATACTCACTT TTGCCCTTTA GAAGGGGAAA GCTGGCAAGA TTTTTTACGT AATAACGCTA
     4321 AAAGTTTTAG ATGTGCTTTA CTAAGTCATC GCGATGGAGC AAAAGTACAT TTAGGTACAC
     4381 GGCCTACAGA AAAACAGTAT GAAACTCTCG AAAATCAATT AGCCTTTTTA TGCCAACAAG
     4441 GTTTTTCACT AGAGAATGCA TTATATGCAC TCAGCGCAGT GGGGCATTTT ACTTTAGGTT
     4501 GCGTATTGGA AGATCAAGAG CATCAAGTCG CTAAAGAAGA AAGGGAAACA CCTACTACTG
     4561 ATAGTATGCC GCCATTATTA CGACAAGCTA TCGAATTATT TGATCACCAA GGTGCAGAGC
     4621 CAGCCTTCTT ATTCGGCCTT GAATTGATCA TATGCGGATT AGAAAAACAA CTTAAATGTG
     4681 AAAGTGGGTC TTAA
//