LOCUS       pKD287                  4704 bp ds-DNA     circular     15-FEB-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2998..3005
                     /label="YvcP/PsdR binding site (inverted repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2975..3084
                     /label="PpsdA110"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2858..2962
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    3057..3062
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2963..2974
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     promoter        1968..1987
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4009..4043
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3069..3069
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2021..2401
                     /label="SO_4637"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3085..3102
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1914..1967
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2986..2993
                     /label="YvcP/PsdR binding site (inverted repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2729..2857
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2171..2173
                     /label="D51"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4044..4077
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3103..3822
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1988..2020
                     /label="sRBS (1,000)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3030..3035
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3835..3963
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             4078..4704
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2402..2716
                     /label="yvcP/psdR"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGACGTCTA AGAAACCATT ATTATCATGA
     1861 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGTCCCTAT
     1921 CAGTGATAGA GATTGACATC CCTATCAGTG ATAGAGATAC TGAGCACATC AGCAGGACGC
     1981 ACTGACCCGA GCGACCCGTA TACCCTTTTA GCACGTATAT atgaaagtat taattgttga
     2041 tgacaatcat gatgttatag aaacaatcat ggactattta accctcgaag gcattattgc
     2101 tgactgcgcc tatcacggtg aaagcgccat caatttaata cagcaaaacc attatgacgt
     2161 gatcattatg gatattatga tgcccaaatt agacggtatc cgtacggtca aaaaactgcg
     2221 cgaagagctg ttttgtaata cgccgatttt gtttttaacc gccagagata gcctgcagga
     2281 caaaattgac tcatttacct cgggcggtga tgacttttta aataaaccct tcgccatgga
     2341 agaactgtgt ctgcgtctac ggtcactgca taatcgcggg ccgcgtttag atgtaggcat
     2401 aGTGGAGCTT GGCGGGCTGA CGATTTATCC TGATCAGAAT GAGGCCGAAT GGAACAGCGT
     2461 CCGGATTTTG TTTTCCCAAA AAGAATTCCA GCTTCTCTCG ATTTTTGTGA GGGAGCATAA
     2521 AAAAATTGTC TCTCGTGATG AACTATTGGA GGCGTTATGG GATGACGTTG ATTTTGTCGA
     2581 TGACAATACG TTAACGGTCA ATGTCAATCG GCTGCGGAGA AAACTGGAGA ATGCAGGACT
     2641 GACAGATTGC ATTTCCACAA TCAGAGGACA AGGTTATCAA TTTCAAGTCA ATCGGAAGGA
     2701 TGAAGCGGAA TGTTAATAAT AATCTAGACC AGGCATCAAA TAAAACGAAA GGCTCAGTCG
     2761 AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCTA CTAGAGTCAC
     2821 ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC GTTTATAGTA AATCACTGCA TAATTCGTGT
     2881 CGCTCAAGGC GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG
     2941 GCAAATATTC TGAAATGAGC TGGTTAGCTA GTCACACCCT CGTGAATGTG ACAGCATTGT
     3001 AAGATTGGGG AGCGGAATTG CAAGAAAGTT CGATGGGAGG ATGCTGACTT CCTTTTTATA
     3061 ATAAAGAAAA AGGAGGAGCA GAACAAAGAG GAGAAATACT AGATGCGTAA AGGCGAAGAG
     3121 CTGTTCACTG GTGTCGTCCC TATTCTGGTG GAACTGGATG GTGATGTCAA CGGTCATAAG
     3181 TTTTCCGTGC GTGGCGAGGG TGAAGGTGAC GCAACTAATG GTAAACTGAC GCTGAAGTTC
     3241 ATCTGTACTA CTGGTAAACT GCCGGTACCT TGGCCGACTC TGGTAACGAC GCTGACTTAT
     3301 GGTGTTCAGT GCTTTGCTCG TTATCCGGAC CATATGAAGC AGCATGACTT CTTCAAGTCC
     3361 GCCATGCCGG AAGGCTATGT GCAGGAACGC ACGATTTCCT TTAAGGATGA CGGCACGTAC
     3421 AAAACGCGTG CGGAAGTGAA ATTTGAAGGC GATACCCTGG TAAACCGCAT TGAGCTGAAA
     3481 GGCATTGACT TTAAAGAAGA CGGCAATATC CTGGGCCATA AGCTGGAATA CAATTTTAAC
     3541 AGCCACAATG TTTACATCAC CGCCGATAAA CAAAAAAATG GCATTAAAGC GAATTTTAAA
     3601 ATTCGCCACA ACGTGGAGGA TGGCAGCGTG CAGCTGGCTG ATCACTACCA GCAAAACACT
     3661 CCAATCGGTG ATGGTCCTGT TCTGCTGCCA GACAATCACT ATCTGAGCAC GCAAAGCGTT
     3721 CTGTCTAAAG ATCCGAACGA GAAACGCGAT CATATGGTTC TGCTGGAGTT CGTAACCGCA
     3781 GCGGGCATCA CGCATGGTAT GGATGAACTG TACAAATGAT GATAATAATC TAGACCAGGC
     3841 ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT
     3901 CGGTGAACGC TCTCTACTAG AGTCACACTG GCTCACCTTC GGGTGGGCCT TTCTGCGTTT
     3961 ATAGGTACCC GTGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTTT TACGGCTAGC
     4021 TCAGTCCTAG GTATAGTGCT AGCCCAGCCA GAGAAACACT CTTTAACAGG GGGTAGTATG
     4081 ATGTCTCGTT TAGATAAAAG TAAAGTGATT AACAGCGCAT TAGAGCTGCT TAATGAGGTC
     4141 GGAATCGAAG GTTTAACAAC CCGTAAACTC GCCCAGAAGC TAGGTGTAGA GCAGCCTACA
     4201 TTGTATTGGC ATGTAAAAAA TAAGCGGGCT TTGCTCGACG CCTTAGCCAT TGAGATGTTA
     4261 GATAGGCACC ATACTCACTT TTGCCCTTTA GAAGGGGAAA GCTGGCAAGA TTTTTTACGT
     4321 AATAACGCTA AAAGTTTTAG ATGTGCTTTA CTAAGTCATC GCGATGGAGC AAAAGTACAT
     4381 TTAGGTACAC GGCCTACAGA AAAACAGTAT GAAACTCTCG AAAATCAATT AGCCTTTTTA
     4441 TGCCAACAAG GTTTTTCACT AGAGAATGCA TTATATGCAC TCAGCGCAGT GGGGCATTTT
     4501 ACTTTAGGTT GCGTATTGGA AGATCAAGAG CATCAAGTCG CTAAAGAAGA AAGGGAAACA
     4561 CCTACTACTG ATAGTATGCC GCCATTATTA CGACAAGCTA TCGAATTATT TGATCACCAA
     4621 GGTGCAGAGC CAGCCTTCTT ATTCGGCCTT GAATTGATCA TATGCGGATT AGAAAAACAA
     4681 CTTAAATGTG AAAGTGGGTC TTAA
//