LOCUS       pSR357.24               4677 bp ds-DNA     circular     05-JUL-2016
DEFINITION  .
KEYWORDS    "Status:Template"                                                  
FEATURES             Location/Qualifiers
     misc_feature    complement(1681..1686)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1658..1663)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2920..2925
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2394..2579
                     /label="ydfI (NarL-FixJ; Bsu)"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    2863..2882
                     /label="YdfI binding site (inverted repeat)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      4573..4677
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2838..2952
                     /label="PydfJ115"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             942..1601
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2721..2825
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1809..1882
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3912..3945
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2936..2936
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      820..925
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2953..2970
                     /label="sRBS (4,296)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2592..2720
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2897..2902
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1664..1669)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2905..2910
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3703..3831
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2917..2917
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2883..2888
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3036..3057
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1863..1863
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1730..1762
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3877..3911
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1883..1916
                     /label="sRBS 918.46"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4015..4038
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3946..4572
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            1917..2393
                     /label="NarL (MG1655)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1559..1583
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1828..1833
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1640..1645)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2826..2837
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    32..53
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(133..815)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1633..1633)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1651..1651)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1851..1856
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4213..4235
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2971..3690
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 CCTAGGCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
       61 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
      121 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      181 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      241 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      301 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG
      361 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      421 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      481 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      541 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
      601 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      661 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
      721 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
      781 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGACTAGT GCTTGGATTC TCACCAATAA
      841 AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA
      901 CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC
      961 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG
     1021 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
     1081 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
     1141 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
     1201 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
     1261 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
     1321 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
     1381 CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
     1441 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
     1501 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
     1561 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
     1621 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
     1681 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA
     1741 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC
     1801 ACCTCGAGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
     1861 ACATCAGCAG GACGCACTGA CCTAAGGATT TTTTCTAGAA GAATTATATA CAACAAatga
     1921 gtaatcagga accggctact atcctgctga ttgacgatca cccgatgctg cgaactggcg
     1981 taaaacagct tatcagtatg gcaccagata tcaccgtggt tggcgaagcg agtaatggcg
     2041 aacagggtat tgaactggcg gagtctcttg atcccgatct gatcctgtta gatctcaata
     2101 tgcccggcat gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctcagggc
     2161 gcattgtggt attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg
     2221 gcgcggatgg ctatctgtta aaagatatgg aaccggaaga tctgctgaaa gcattgcatc
     2281 aggcagctgc tggcgaaatg gtattaagcg aagcattaac gcctgttctg gccgccagct
     2341 tgcgcgctaa ccgtgccact actgagcgcg atgttaacca gttaacccca cgcGAAGTCA
     2401 TTGTTCTAAA AGCAATTGCT AAAGGTCTTA AAAGCAAAGC GATTGCCTTT GATTTGGGCG
     2461 TCTCTGAGCG AACAGTAAAG TCCAGATTAA CGTCCATTTA CAATAAATTA GGCGCGAATT
     2521 CAAGAACTGA GGCAGTAACG ATTGCCATGC AAAAAGGTAT TCTGACAATA GACAACTAAT
     2581 AATAATCTAG ACCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG
     2641 TTTTATCTGT TGTTTGTCGG TGAACGCTCT CTACTAGAGT CACACTGGCT CACCTTCGGG
     2701 TGGGCCTTTC TGCGTTTATA GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC
     2761 CGTTCTGGAT AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG
     2821 AGCTGGTTAG CTAGTCAACT GCATATTTGA AAATTGCCCA AACGTACATG CCCGAATGTA
     2881 CGTTTTTTTC ATTTCATTGT CAACTACAAT GAGAAAGAAT GTGATCAAGC AATGTGTTGA
     2941 AAGGAGATTA TCAAAGAGGA GAAATACTAG ATGCGTAAAG GCGAAGAGCT GTTCACTGGT
     3001 GTCGTCCCTA TTCTGGTGGA ACTGGATGGT GATGTCAACG GTCATAAGTT TTCCGTGCGT
     3061 GGCGAGGGTG AAGGTGACGC AACTAATGGT AAACTGACGC TGAAGTTCAT CTGTACTACT
     3121 GGTAAACTGC CGGTACCTTG GCCGACTCTG GTAACGACGC TGACTTATGG TGTTCAGTGC
     3181 TTTGCTCGTT ATCCGGACCA TATGAAGCAG CATGACTTCT TCAAGTCCGC CATGCCGGAA
     3241 GGCTATGTGC AGGAACGCAC GATTTCCTTT AAGGATGACG GCACGTACAA AACGCGTGCG
     3301 GAAGTGAAAT TTGAAGGCGA TACCCTGGTA AACCGCATTG AGCTGAAAGG CATTGACTTT
     3361 AAAGAAGACG GCAATATCCT GGGCCATAAG CTGGAATACA ATTTTAACAG CCACAATGTT
     3421 TACATCACCG CCGATAAACA AAAAAATGGC ATTAAAGCGA ATTTTAAAAT TCGCCACAAC
     3481 GTGGAGGATG GCAGCGTGCA GCTGGCTGAT CACTACCAGC AAAACACTCC AATCGGTGAT
     3541 GGTCCTGTTC TGCTGCCAGA CAATCACTAT CTGAGCACGC AAAGCGTTCT GTCTAAAGAT
     3601 CCGAACGAGA AACGCGATCA TATGGTTCTG CTGGAGTTCG TAACCGCAGC GGGCATCACG
     3661 CATGGTATGG ATGAACTGTA CAAATGATGA TAATAATCTA GACCAGGCAT CAAATAAAAC
     3721 GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC
     3781 TCTACTAGAG TCACACTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTTAT AGGTACCCGT
     3841 GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTTTTA CGGCTAGCTC AGTCCTAGGT
     3901 ATAGTGCTAG CCCAGCCAGA GAAACACTCT TTAACAGGGG GTAGTATGAT GTCTCGTTTA
     3961 GATAAAAGTA AAGTGATTAA CAGCGCATTA GAGCTGCTTA ATGAGGTCGG AATCGAAGGT
     4021 TTAACAACCC GTAAACTCGC CCAGAAGCTA GGTGTAGAGC AGCCTACATT GTATTGGCAT
     4081 GTAAAAAATA AGCGGGCTTT GCTCGACGCC TTAGCCATTG AGATGTTAGA TAGGCACCAT
     4141 ACTCACTTTT GCCCTTTAGA AGGGGAAAGC TGGCAAGATT TTTTACGTAA TAACGCTAAA
     4201 AGTTTTAGAT GTGCTTTACT AAGTCATCGC GATGGAGCAA AAGTACATTT AGGTACACGG
     4261 CCTACAGAAA AACAGTATGA AACTCTCGAA AATCAATTAG CCTTTTTATG CCAACAAGGT
     4321 TTTTCACTAG AGAATGCATT ATATGCACTC AGCGCAGTGG GGCATTTTAC TTTAGGTTGC
     4381 GTATTGGAAG ATCAAGAGCA TCAAGTCGCT AAAGAAGAAA GGGAAACACC TACTACTGAT
     4441 AGTATGCCGC CATTATTACG ACAAGCTATC GAATTATTTG ATCACCAAGG TGCAGAGCCA
     4501 GCCTTCTTAT TCGGCCTTGA ATTGATCATA TGCGGATTAG AAAAACAACT TAAATGTGAA
     4561 AGTGGGTCTT AAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT
     4621 TTATCTGTTG TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG CCCTAGA
//