LOCUS       pSR358                  4671 bp ds-DNA     circular     29-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2837..2842
                     /label="LiaR binding site (direct repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(133..815)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1828..1833
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2890..2895
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2310..2567
                     /label="liaR (NarL-FixJ; Bsu)"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    2855..2860
                     /label="LiaR binding site (direct repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1658..1663)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2580..2708
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     gene            1917..2309
                     /label="NarL (MG1655)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1664..1669)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1809..1882
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3030..3051
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1681..1686)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      820..925
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    3697..3825
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1883..1916
                     /label="sRBS 918.46"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1730..1762
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1633..1633)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2909..2914
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4009..4032
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1851..1856
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2922..2922
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1651..1651)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2947..2964
                     /label="sRBS (2,393)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1559..1583
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4207..4229
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             942..1601
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    32..53
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3906..3939
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2814..2825
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    3871..3905
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3940..4566
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1863..1863
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1640..1645)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2826..2946
                     /label="PliaI121"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2709..2813
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2965..3684
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      4567..4671
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
ORIGIN
        1 CCTAGGCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
       61 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
      121 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      181 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      241 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      301 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG
      361 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      421 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      481 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      541 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
      601 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      661 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
      721 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
      781 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGACTAGT GCTTGGATTC TCACCAATAA
      841 AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA
      901 CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC
      961 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG
     1021 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
     1081 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
     1141 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
     1201 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
     1261 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
     1321 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
     1381 CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
     1441 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
     1501 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
     1561 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
     1621 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
     1681 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA
     1741 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC
     1801 ACCTCGAGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
     1861 ACATCAGCAG GACGCACTGA CCTAAGGATT TTTTCTAGAA GAATTATATA CAACAAatga
     1921 gtaatcagga accggctact atcctgctga ttgacgatca cccgatgctg cgaactggcg
     1981 taaaacagct tatcagtatg gcaccagata tcaccgtggt tggcgaagcg agtaatggcg
     2041 aacagggtat tgaactggcg gagtctcttg atcccgatct gatcctgtta gatctcaata
     2101 tgcccggcat gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctcagggc
     2161 gcattgtggt attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg
     2221 gcgcggatgg ctatctgtta aaagatatgg aaccggaaga tctgctgaaa gcattgcatc
     2281 aggcagctgc tggcgaaatg gtattaagcT CAAAAGTGGC GGGAAAAGTA TTATCCAGGC
     2341 TGCGCCACTC AGGTGAAAAC GCGCTCCCGC ATGAATCGCT TACAAAACGG GAGCTCGAAA
     2401 TACTCTGCCT GATCGCAGAA GGAAAGACAA ACAAAGAAAT AGGCGAGGAA CTGTTTATTA
     2461 CGATTAAAAC AGTCAAAACA CATATTACGA ATATTTTATC AAAGCTGGAT GTCAGTGACC
     2521 GGACGCAGGC GGCGGTGTAC GCACACCGAA ATCATCTCGT GAATTAGTAA TAATCTAGAC
     2581 CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG
     2641 TTTGTCGGTG AACGCTCTCT ACTAGAGTCA CACTGGCTCA CCTTCGGGTG GGCCTTTCTG
     2701 CGTTTATAGT AAATCACTGC ATAATTCGTG TCGCTCAAGG CGCACTCCCG TTCTGGATAA
     2761 TGTTTTTTGC GCCGACATCA TAACGGTTCT GGCAAATATT CTGAAATGAG CTGGTTAGCT
     2821 AGTCAGTGCC AATGGGTCCG GTGCGAGATA CGACTCCGGT CTTATATAAA AATCAATCTC
     2881 TGATTCGTTT TGCATATCTT CCAACTTGTA TAAGATGAAG ACAAGGAAAA CGAAAGGAGG
     2941 ATCTGCAAAG AGGAGAAATA CTAGATGCGT AAAGGCGAAG AGCTGTTCAC TGGTGTCGTC
     3001 CCTATTCTGG TGGAACTGGA TGGTGATGTC AACGGTCATA AGTTTTCCGT GCGTGGCGAG
     3061 GGTGAAGGTG ACGCAACTAA TGGTAAACTG ACGCTGAAGT TCATCTGTAC TACTGGTAAA
     3121 CTGCCGGTAC CTTGGCCGAC TCTGGTAACG ACGCTGACTT ATGGTGTTCA GTGCTTTGCT
     3181 CGTTATCCGG ACCATATGAA GCAGCATGAC TTCTTCAAGT CCGCCATGCC GGAAGGCTAT
     3241 GTGCAGGAAC GCACGATTTC CTTTAAGGAT GACGGCACGT ACAAAACGCG TGCGGAAGTG
     3301 AAATTTGAAG GCGATACCCT GGTAAACCGC ATTGAGCTGA AAGGCATTGA CTTTAAAGAA
     3361 GACGGCAATA TCCTGGGCCA TAAGCTGGAA TACAATTTTA ACAGCCACAA TGTTTACATC
     3421 ACCGCCGATA AACAAAAAAA TGGCATTAAA GCGAATTTTA AAATTCGCCA CAACGTGGAG
     3481 GATGGCAGCG TGCAGCTGGC TGATCACTAC CAGCAAAACA CTCCAATCGG TGATGGTCCT
     3541 GTTCTGCTGC CAGACAATCA CTATCTGAGC ACGCAAAGCG TTCTGTCTAA AGATCCGAAC
     3601 GAGAAACGCG ATCATATGGT TCTGCTGGAG TTCGTAACCG CAGCGGGCAT CACGCATGGT
     3661 ATGGATGAAC TGTACAAATG ATGATAATAA TCTAGACCAG GCATCAAATA AAACGAAAGG
     3721 CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCTACT
     3781 AGAGTCACAC TGGCTCACCT TCGGGTGGGC CTTTCTGCGT TTATAGGTAC CCGTGGATCC
     3841 TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT TTTACGGCTA GCTCAGTCCT AGGTATAGTG
     3901 CTAGCCCAGC CAGAGAAACA CTCTTTAACA GGGGGTAGTA TGATGTCTCG TTTAGATAAA
     3961 AGTAAAGTGA TTAACAGCGC ATTAGAGCTG CTTAATGAGG TCGGAATCGA AGGTTTAACA
     4021 ACCCGTAAAC TCGCCCAGAA GCTAGGTGTA GAGCAGCCTA CATTGTATTG GCATGTAAAA
     4081 AATAAGCGGG CTTTGCTCGA CGCCTTAGCC ATTGAGATGT TAGATAGGCA CCATACTCAC
     4141 TTTTGCCCTT TAGAAGGGGA AAGCTGGCAA GATTTTTTAC GTAATAACGC TAAAAGTTTT
     4201 AGATGTGCTT TACTAAGTCA TCGCGATGGA GCAAAAGTAC ATTTAGGTAC ACGGCCTACA
     4261 GAAAAACAGT ATGAAACTCT CGAAAATCAA TTAGCCTTTT TATGCCAACA AGGTTTTTCA
     4321 CTAGAGAATG CATTATATGC ACTCAGCGCA GTGGGGCATT TTACTTTAGG TTGCGTATTG
     4381 GAAGATCAAG AGCATCAAGT CGCTAAAGAA GAAAGGGAAA CACCTACTAC TGATAGTATG
     4441 CCGCCATTAT TACGACAAGC TATCGAATTA TTTGATCACC AAGGTGCAGA GCCAGCCTTC
     4501 TTATTCGGCC TTGAATTGAT CATATGCGGA TTAGAAAAAC AACTTAAATG TGAAAGTGGG
     4561 TCTTAAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT
     4621 GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGCCCTAG A
//