LOCUS       pSR359.4                4643 bp ds-DNA     circular     12-SEP-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2937..3656
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1633..1633)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1863..1863
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1828..1833
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2915..2915
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2863..2882
                     /label="UhpB binding site (inverted repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(133..815)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     RBS             1883..1916
                     /label="sRBS 918.46"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3002..3023
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1559..1583
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      820..925
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2808..2819
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    3669..3797
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2703..2807
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3878..3911
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2902..2907
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    32..53
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4179..4201
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            1917..2393
                     /label="NarL (MG1655)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1730..1762
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1640..1645)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4539..4643
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2840..2859
                     /label="UhpB binding site (inverted repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1809..1882
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3981..4004
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3912..4538
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2820..2918
                     /label="PuhpT99"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1651..1651)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2883..2888
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2919..2936
                     /label="sRBS (813)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1658..1663)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2394..2561
                     /label="UhpA (NarL-FixJ; Eco)"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    1851..1856
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1681..1686)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3843..3877
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1664..1669)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2574..2702
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             942..1601
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 CCTAGGCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
       61 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
      121 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      181 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      241 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      301 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG
      361 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      421 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      481 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      541 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
      601 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      661 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
      721 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
      781 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGACTAGT GCTTGGATTC TCACCAATAA
      841 AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA
      901 CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC
      961 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG
     1021 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
     1081 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
     1141 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
     1201 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
     1261 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
     1321 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
     1381 CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
     1441 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
     1501 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
     1561 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
     1621 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
     1681 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA
     1741 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC
     1801 ACCTCGAGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
     1861 ACATCAGCAG GACGCACTGA CCTAAGGATT TTTTCTAGAA GAATTATATA CAACAAatga
     1921 gtaatcagga accggctact atcctgctga ttgacgatca cccgatgctg cgaactggcg
     1981 taaaacagct tatcagtatg gcaccagata tcaccgtggt tggcgaagcg agtaatggcg
     2041 aacagggtat tgaactggcg gagtctcttg atcccgatct gatcctgtta gatctcaata
     2101 tgcccggcat gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctcagggc
     2161 gcattgtggt attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg
     2221 gcgcggatgg ctatctgtta aaagatatgg aaccggaaga tctgctgaaa gcattgcatc
     2281 aggcagctgc tggcgaaatg gtattaagcg aagcattaac gcctgttctg gccgccagct
     2341 tgcgcgctaa ccgtgccact actgagcgcg atgttaacca gttaacccca cgcGAACGCC
     2401 AGGTGGCGGA AAAACTGGCG CAAGGAATGG CGGTGAAAGA GATTGCCGCC GAACTGGGCT
     2461 TGTCACCGAA AACGGTACAC GTCCATCGCG CCAATCTGAT GGAAAAACTG GGCGTCAGTA
     2521 ACGACGTAGA GCTGGCGCGC CGCATGTTTG ATGGCTGGTG ATAATAATCT AGACCAGGCA
     2581 TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC
     2641 GGTGAACGCT CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA
     2701 TAGTAAATCA CTGCATAATT CGTGTCGCTC AAGGCGCACT CCCGTTCTGG ATAATGTTTT
     2761 TTGCGCCGAC ATCATAACGG TTCTGGCAAA TATTCTGAAA TGAGCTGGTT AGCTAGTCAT
     2821 TTCACTTCAT ATCCGGCAAA ACTAAGAAAT TTTCCAGGTT TTGCCTGGAC GCTATCTCAG
     2881 GCCTGATTTG CTGCTGATTT TTACAATGCA TGCCTCACAA AGAGGAGAAA TACTAGATGC
     2941 GTAAAGGCGA AGAGCTGTTC ACTGGTGTCG TCCCTATTCT GGTGGAACTG GATGGTGATG
     3001 TCAACGGTCA TAAGTTTTCC GTGCGTGGCG AGGGTGAAGG TGACGCAACT AATGGTAAAC
     3061 TGACGCTGAA GTTCATCTGT ACTACTGGTA AACTGCCGGT ACCTTGGCCG ACTCTGGTAA
     3121 CGACGCTGAC TTATGGTGTT CAGTGCTTTG CTCGTTATCC GGACCATATG AAGCAGCATG
     3181 ACTTCTTCAA GTCCGCCATG CCGGAAGGCT ATGTGCAGGA ACGCACGATT TCCTTTAAGG
     3241 ATGACGGCAC GTACAAAACG CGTGCGGAAG TGAAATTTGA AGGCGATACC CTGGTAAACC
     3301 GCATTGAGCT GAAAGGCATT GACTTTAAAG AAGACGGCAA TATCCTGGGC CATAAGCTGG
     3361 AATACAATTT TAACAGCCAC AATGTTTACA TCACCGCCGA TAAACAAAAA AATGGCATTA
     3421 AAGCGAATTT TAAAATTCGC CACAACGTGG AGGATGGCAG CGTGCAGCTG GCTGATCACT
     3481 ACCAGCAAAA CACTCCAATC GGTGATGGTC CTGTTCTGCT GCCAGACAAT CACTATCTGA
     3541 GCACGCAAAG CGTTCTGTCT AAAGATCCGA ACGAGAAACG CGATCATATG GTTCTGCTGG
     3601 AGTTCGTAAC CGCAGCGGGC ATCACGCATG GTATGGATGA ACTGTACAAA TGATGATAAT
     3661 AATCTAGACC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT
     3721 TATCTGTTGT TTGTCGGTGA ACGCTCTCTA CTAGAGTCAC ACTGGCTCAC CTTCGGGTGG
     3781 GCCTTTCTGC GTTTATAGGT ACCCGTGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC
     3841 TTTTTACGGC TAGCTCAGTC CTAGGTATAG TGCTAGCCCA GCCAGAGAAA CACTCTTTAA
     3901 CAGGGGGTAG TATGATGTCT CGTTTAGATA AAAGTAAAGT GATTAACAGC GCATTAGAGC
     3961 TGCTTAATGA GGTCGGAATC GAAGGTTTAA CAACCCGTAA ACTCGCCCAG AAGCTAGGTG
     4021 TAGAGCAGCC TACATTGTAT TGGCATGTAA AAAATAAGCG GGCTTTGCTC GACGCCTTAG
     4081 CCATTGAGAT GTTAGATAGG CACCATACTC ACTTTTGCCC TTTAGAAGGG GAAAGCTGGC
     4141 AAGATTTTTT ACGTAATAAC GCTAAAAGTT TTAGATGTGC TTTACTAAGT CATCGCGATG
     4201 GAGCAAAAGT ACATTTAGGT ACACGGCCTA CAGAAAAACA GTATGAAACT CTCGAAAATC
     4261 AATTAGCCTT TTTATGCCAA CAAGGTTTTT CACTAGAGAA TGCATTATAT GCACTCAGCG
     4321 CAGTGGGGCA TTTTACTTTA GGTTGCGTAT TGGAAGATCA AGAGCATCAA GTCGCTAAAG
     4381 AAGAAAGGGA AACACCTACT ACTGATAGTA TGCCGCCATT ATTACGACAA GCTATCGAAT
     4441 TATTTGATCA CCAAGGTGCA GAGCCAGCCT TCTTATTCGG CCTTGAATTG ATCATATGCG
     4501 GATTAGAAAA ACAACTTAAA TGTGAAAGTG GGTCTTAAGG CATCAAATAA AACGAAAGGC
     4561 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG
     4621 TAGGACAAAT CCGCCGCCCT AGA
//