LOCUS       pSR322.3                4651 bp ds-DNA     circular     29-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3655..3688
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1433..1438)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1597..1602
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2606..2625
                     /label="YdfI binding site (inverted repeat)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2640..2645
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2050..2322
                     /label="ydfI (NarL-FixJ; Bsu)"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    1328..1352
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1687..2049
                     /label="UhpA (NarL-FixJ; Eco)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(4553..584)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      589..694
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2464..2568
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             711..1370
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1402..1402)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2127..2127
                     /label="G->T Silent"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2648..2653
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1620..1625
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1499..1531
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4316..4420
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    complement(1409..1414)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2696..2713
                     /label="sRBS (4,296)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2679..2679
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2581..2695
                     /label="PydfJ115"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3446..3574
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2335..2463
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2663..2668
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3758..3781
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3620..3654
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1427..1432)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3689..4315
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1450..1455)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2714..3433
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4452..4473
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2569..2580
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    1632..1632
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3956..3978
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2779..2800
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2660..2660
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1652..1686
                     /label="sRBS (1,039)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1578..1651
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1420..1420)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2626..2631
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
       61 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC
      121 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
      181 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
      241 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
      301 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
      361 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
      421 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
      481 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
      541 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGACTAGTG CTTGGATTCT
      601 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
      661 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCGAGCT CGATATCAAA TTACGCCCCG
      721 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA
      781 TCACAGACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA
      841 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA
      901 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC
      961 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT
     1021 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC
     1081 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC
     1141 ATTGCCATAC GAAATTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC
     1201 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA
     1261 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA
     1321 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC
     1381 TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT TATTTCATTA
     1441 TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC ATTTTCGCCA GATATCGACG
     1501 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
     1561 TTCGTCTTCA CCTCGAGTCC CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG
     1621 ATACTGAGCA CATCAGCAGG ACGCACTGAC CAGTTCTAAG ACGTAAAAAA GTAGATAGGA
     1681 CGCCATATGA TCACCGTTGC CCTTATAGAC GATCACCTCA TCGTCCGCTC CGGCTTTGCG
     1741 CAGCTGCTGG GGCTGGAACC TGATTTGCAG GTAGTTGCCG AGTTTGGTTC GGGGCGCGAG
     1801 GCGCTGGCGG GGCTGCCGGG GCGCGGTGTG CAGGTGTGTA TTTGCGATAT CTCCATGCCC
     1861 GATATCTCCG GTCTGGAGCT GCTAAGCCAG CTGCCGAAAG GTATGGCGAC GATTATGCTC
     1921 TCCGTTCACG ACAGTCCTGC GCTGGTTGAG CAGGCGCTTA ACGCGGGGGC ACGCGGCTTT
     1981 CTTTCCAAAC GCTGTAGCCC GGATGAACTC ATTGCTGCGG TGCATACGGT TGCCACGGGC
     2041 GGCTGTTATT TGCAGCCTGA TATCCTTAAA CGTCTGCAAG AAATCCAATT TGAGCGGATG
     2101 AAAAAGCAGC GCAATGAGAC GCAGCTGACA GAAAAGGAAG TCATTGTTCT AAAAGCAATT
     2161 GCTAAAGGTC TTAAAAGCAA AGCGATTGCC TTTGATTTGG GCGTCTCTGA GCGAACAGTA
     2221 AAGTCCAGAT TAACGTCCAT TTACAATAAA TTAGGCGCGA ATTCAAGAAC TGAGGCAGTA
     2281 ACGATTGCCA TGCAAAAAGG TATTCTGACA ATAGACAACT AATAATAATC TAGACCAGGC
     2341 ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT
     2401 CGGTGAACGC TCTCTACTAG AGTCACACTG GCTCACCTTC GGGTGGGCCT TTCTGCGTTT
     2461 ATAGTAAATC ACTGCATAAT TCGTGTCGCT CAAGGCGCAC TCCCGTTCTG GATAATGTTT
     2521 TTTGCGCCGA CATCATAACG GTTCTGGCAA ATATTCTGAA ATGAGCTGGT TAGCTAGTCA
     2581 ACTGCATATT TGAAAATTGC CCAAACGTAC ATGCCCGAAT GTACGTTTTT TTCATTTCAT
     2641 TGTCAACTAC AATGAGAAAG AATGTGATCA AGCAATGTGT TGAAAGGAGA TTATCAAAGA
     2701 GGAGAAATAC TAGATGCGTA AAGGCGAAGA GCTGTTCACT GGTGTCGTCC CTATTCTGGT
     2761 GGAACTGGAT GGTGATGTCA ACGGTCATAA GTTTTCCGTG CGTGGCGAGG GTGAAGGTGA
     2821 CGCAACTAAT GGTAAACTGA CGCTGAAGTT CATCTGTACT ACTGGTAAAC TGCCGGTACC
     2881 TTGGCCGACT CTGGTAACGA CGCTGACTTA TGGTGTTCAG TGCTTTGCTC GTTATCCGGA
     2941 CCATATGAAG CAGCATGACT TCTTCAAGTC CGCCATGCCG GAAGGCTATG TGCAGGAACG
     3001 CACGATTTCC TTTAAGGATG ACGGCACGTA CAAAACGCGT GCGGAAGTGA AATTTGAAGG
     3061 CGATACCCTG GTAAACCGCA TTGAGCTGAA AGGCATTGAC TTTAAAGAAG ACGGCAATAT
     3121 CCTGGGCCAT AAGCTGGAAT ACAATTTTAA CAGCCACAAT GTTTACATCA CCGCCGATAA
     3181 ACAAAAAAAT GGCATTAAAG CGAATTTTAA AATTCGCCAC AACGTGGAGG ATGGCAGCGT
     3241 GCAGCTGGCT GATCACTACC AGCAAAACAC TCCAATCGGT GATGGTCCTG TTCTGCTGCC
     3301 AGACAATCAC TATCTGAGCA CGCAAAGCGT TCTGTCTAAA GATCCGAACG AGAAACGCGA
     3361 TCATATGGTT CTGCTGGAGT TCGTAACCGC AGCGGGCATC ACGCATGGTA TGGATGAACT
     3421 GTACAAATGA TGATAATAAT CTAGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA
     3481 GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT
     3541 GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATAGGTACC CGTGGATCCT CTAGAGTCGA
     3601 CCTGCAGGCA TGCAAGCTTT TTACGGCTAG CTCAGTCCTA GGTATAGTGC TAGCCCAGCC
     3661 AGAGAAACAC TCTTTAACAG GGGGTAGTAT GATGTCTCGT TTAGATAAAA GTAAAGTGAT
     3721 TAACAGCGCA TTAGAGCTGC TTAATGAGGT CGGAATCGAA GGTTTAACAA CCCGTAAACT
     3781 CGCCCAGAAG CTAGGTGTAG AGCAGCCTAC ATTGTATTGG CATGTAAAAA ATAAGCGGGC
     3841 TTTGCTCGAC GCCTTAGCCA TTGAGATGTT AGATAGGCAC CATACTCACT TTTGCCCTTT
     3901 AGAAGGGGAA AGCTGGCAAG ATTTTTTACG TAATAACGCT AAAAGTTTTA GATGTGCTTT
     3961 ACTAAGTCAT CGCGATGGAG CAAAAGTACA TTTAGGTACA CGGCCTACAG AAAAACAGTA
     4021 TGAAACTCTC GAAAATCAAT TAGCCTTTTT ATGCCAACAA GGTTTTTCAC TAGAGAATGC
     4081 ATTATATGCA CTCAGCGCAG TGGGGCATTT TACTTTAGGT TGCGTATTGG AAGATCAAGA
     4141 GCATCAAGTC GCTAAAGAAG AAAGGGAAAC ACCTACTACT GATAGTATGC CGCCATTATT
     4201 ACGACAAGCT ATCGAATTAT TTGATCACCA AGGTGCAGAG CCAGCCTTCT TATTCGGCCT
     4261 TGAATTGATC ATATGCGGAT TAGAAAAACA ACTTAAATGT GAAAGTGGGT CTTAAGGCAT
     4321 CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG
     4381 GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGCCCTAGA CCTAGGCGTT CGGCTGCGGC
     4441 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG
     4501 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
     4561 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
     4621 GTCAGAGGTG GCGAAACCCG ACAGGACTAT A
//