LOCUS       pSR322.4                4624 bp ds-DNA     circular     29-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1620..1625
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3929..3951
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1652..1686
                     /label="sRBS (1,039)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2110..2295
                     /label="ydfI (NarL-FixJ; Bsu)"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    complement(1420..1420)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2752..2773
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1409..1414)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2599..2604
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1632..1632
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3662..4288
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2621..2626
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2579..2598
                     /label="YdfI binding site (inverted repeat)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2542..2553
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    2308..2436
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2554..2668
                     /label="PydfJ115"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1402..1402)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2633..2633
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1597..1602
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2669..2686
                     /label="sRBS (4,296)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             711..1370
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1328..1352
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2636..2641
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3731..3754
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4425..4446
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(4526..584)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1578..1651
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      4289..4393
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    3419..3547
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3628..3661
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2687..3406
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1687..2109
                     /label="UhpA (NarL-FixJ; Eco)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3593..3627
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1450..1455)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1499..1531
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1427..1432)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2437..2541
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      589..694
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2652..2652
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1433..1438)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2613..2618
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
       61 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC
      121 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
      181 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
      241 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
      301 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
      361 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
      421 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
      481 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
      541 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGACTAGTG CTTGGATTCT
      601 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
      661 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCGAGCT CGATATCAAA TTACGCCCCG
      721 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA
      781 TCACAGACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA
      841 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA
      901 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC
      961 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT
     1021 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC
     1081 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC
     1141 ATTGCCATAC GAAATTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC
     1201 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA
     1261 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA
     1321 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC
     1381 TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT TATTTCATTA
     1441 TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC ATTTTCGCCA GATATCGACG
     1501 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
     1561 TTCGTCTTCA CCTCGAGTCC CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG
     1621 ATACTGAGCA CATCAGCAGG ACGCACTGAC CAGTTCTAAG ACGTAAAAAA GTAGATAGGA
     1681 CGCCATATGA TCACCGTTGC CCTTATAGAC GATCACCTCA TCGTCCGCTC CGGCTTTGCG
     1741 CAGCTGCTGG GGCTGGAACC TGATTTGCAG GTAGTTGCCG AGTTTGGTTC GGGGCGCGAG
     1801 GCGCTGGCGG GGCTGCCGGG GCGCGGTGTG CAGGTGTGTA TTTGCGATAT CTCCATGCCC
     1861 GATATCTCCG GTCTGGAGCT GCTAAGCCAG CTGCCGAAAG GTATGGCGAC GATTATGCTC
     1921 TCCGTTCACG ACAGTCCTGC GCTGGTTGAG CAGGCGCTTA ACGCGGGGGC ACGCGGCTTT
     1981 CTTTCCAAAC GCTGTAGCCC GGATGAACTC ATTGCTGCGG TGCATACGGT TGCCACGGGC
     2041 GGCTGTTATC TGACGCCGGA TATTGCCATT AAACTGGCAT CCGGTCGTCA GGACCCGCTA
     2101 ACCAAACGTG AAGTCATTGT TCTAAAAGCA ATTGCTAAAG GTCTTAAAAG CAAAGCGATT
     2161 GCCTTTGATT TGGGCGTCTC TGAGCGAACA GTAAAGTCCA GATTAACGTC CATTTACAAT
     2221 AAATTAGGCG CGAATTCAAG AACTGAGGCA GTAACGATTG CCATGCAAAA AGGTATTCTG
     2281 ACAATAGACA ACTAATAATA ATCTAGACCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA
     2341 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA
     2401 CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATAGTAA ATCACTGCAT AATTCGTGTC
     2461 GCTCAAGGCG CACTCCCGTT CTGGATAATG TTTTTTGCGC CGACATCATA ACGGTTCTGG
     2521 CAAATATTCT GAAATGAGCT GGTTAGCTAG TCAACTGCAT ATTTGAAAAT TGCCCAAACG
     2581 TACATGCCCG AATGTACGTT TTTTTCATTT CATTGTCAAC TACAATGAGA AAGAATGTGA
     2641 TCAAGCAATG TGTTGAAAGG AGATTATCAA AGAGGAGAAA TACTAGATGC GTAAAGGCGA
     2701 AGAGCTGTTC ACTGGTGTCG TCCCTATTCT GGTGGAACTG GATGGTGATG TCAACGGTCA
     2761 TAAGTTTTCC GTGCGTGGCG AGGGTGAAGG TGACGCAACT AATGGTAAAC TGACGCTGAA
     2821 GTTCATCTGT ACTACTGGTA AACTGCCGGT ACCTTGGCCG ACTCTGGTAA CGACGCTGAC
     2881 TTATGGTGTT CAGTGCTTTG CTCGTTATCC GGACCATATG AAGCAGCATG ACTTCTTCAA
     2941 GTCCGCCATG CCGGAAGGCT ATGTGCAGGA ACGCACGATT TCCTTTAAGG ATGACGGCAC
     3001 GTACAAAACG CGTGCGGAAG TGAAATTTGA AGGCGATACC CTGGTAAACC GCATTGAGCT
     3061 GAAAGGCATT GACTTTAAAG AAGACGGCAA TATCCTGGGC CATAAGCTGG AATACAATTT
     3121 TAACAGCCAC AATGTTTACA TCACCGCCGA TAAACAAAAA AATGGCATTA AAGCGAATTT
     3181 TAAAATTCGC CACAACGTGG AGGATGGCAG CGTGCAGCTG GCTGATCACT ACCAGCAAAA
     3241 CACTCCAATC GGTGATGGTC CTGTTCTGCT GCCAGACAAT CACTATCTGA GCACGCAAAG
     3301 CGTTCTGTCT AAAGATCCGA ACGAGAAACG CGATCATATG GTTCTGCTGG AGTTCGTAAC
     3361 CGCAGCGGGC ATCACGCATG GTATGGATGA ACTGTACAAA TGATGATAAT AATCTAGACC
     3421 AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT
     3481 TTGTCGGTGA ACGCTCTCTA CTAGAGTCAC ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC
     3541 GTTTATAGGT ACCCGTGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTTTTACGGC
     3601 TAGCTCAGTC CTAGGTATAG TGCTAGCCCA GCCAGAGAAA CACTCTTTAA CAGGGGGTAG
     3661 TATGATGTCT CGTTTAGATA AAAGTAAAGT GATTAACAGC GCATTAGAGC TGCTTAATGA
     3721 GGTCGGAATC GAAGGTTTAA CAACCCGTAA ACTCGCCCAG AAGCTAGGTG TAGAGCAGCC
     3781 TACATTGTAT TGGCATGTAA AAAATAAGCG GGCTTTGCTC GACGCCTTAG CCATTGAGAT
     3841 GTTAGATAGG CACCATACTC ACTTTTGCCC TTTAGAAGGG GAAAGCTGGC AAGATTTTTT
     3901 ACGTAATAAC GCTAAAAGTT TTAGATGTGC TTTACTAAGT CATCGCGATG GAGCAAAAGT
     3961 ACATTTAGGT ACACGGCCTA CAGAAAAACA GTATGAAACT CTCGAAAATC AATTAGCCTT
     4021 TTTATGCCAA CAAGGTTTTT CACTAGAGAA TGCATTATAT GCACTCAGCG CAGTGGGGCA
     4081 TTTTACTTTA GGTTGCGTAT TGGAAGATCA AGAGCATCAA GTCGCTAAAG AAGAAAGGGA
     4141 AACACCTACT ACTGATAGTA TGCCGCCATT ATTACGACAA GCTATCGAAT TATTTGATCA
     4201 CCAAGGTGCA GAGCCAGCCT TCTTATTCGG CCTTGAATTG ATCATATGCG GATTAGAAAA
     4261 ACAACTTAAA TGTGAAAGTG GGTCTTAAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA
     4321 GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT
     4381 CCGCCGCCCT AGACCTAGGC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
     4441 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
     4501 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
     4561 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
     4621 TATA
//