LOCUS       pMinDis-X513-iNicSNFR3a 6832 bp ds-DNA     circular     29-MAR-2019
DEFINITION  .
ACCESSION   urn:local:.:1506620492729.20                                       
FEATURES             Location/Qualifiers
     misc_feature    931..933
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    799..799
                     /label="MCS"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    545..549
                     /label="CAAAT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     modified_base   1975..1977
                     /label="E174F"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1391..1391
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1882..1884
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1777..2367
                     /label="X513OpuB 107-305"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1030..1041
                     /label="polylinker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    578..584
                     /label="TATA box"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     modified_base   931..933
                     /label="modified_base"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             1330..1764
                     /label="cpsfGFP 1-146"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1987..1988
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1866..1899
                     /label="motif"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     modified_base   1825..1827
                     /label="modified_base"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2..597
                     /label="hCMV promoter/enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2368..2373
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     modified_base   2302..2304
                     /label="modified_base"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      complement(2925..3607)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1765..1773
                     /label="polylinker"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             1042..1311
                     /label="cpsfGFP 149-238"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     modified_base   2272..2274
                     /label="modified_base"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1315..1320
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     modified_base   1987..1989
                     /label="modified_base"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    800..803
                     /label="ligation"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    805..1029
                     /label="X513OpuB 30-106"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     modified_base   1006..1008
                     /label="modified_base"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2167..2169
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(5385..6044)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    848..849
                     /label="Geneious type: Editing History Insertion"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    799..804
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     modified_base   2110..2112
                     /label="modified_base"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1008..1008
                     /label="Geneious type: Editing History Insertion"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2581..2598)
                     /label="r.0.401"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2413..2562
                     /label="PDGFR 513-561"
                     /ApEinfo_revcolor=#a58d2a
                     /ApEinfo_fwdcolor=#a58d2a
     misc_feature    2369..2372
                     /label="ligation"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1005..1006
                     /label="Geneious type: Editing History Deletion"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     637..656
                     /label="T7 promoter/priming site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     modified_base   1030..1032
                     /label="modified_base"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2111..2112
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     modified_base   1792..1794
                     /label="modified_base"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    800..804
                     /label="misc_feature"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     modified_base   2167..2169
                     /label="modified_base"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     modified_base   1390..1392
                     /label="modified_base"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    846..847
                     /label="Geneious type: Editing History Deletion"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    736..798
                     /label="Ig K-Chain"
                     /ApEinfo_revcolor=#845300
                     /ApEinfo_fwdcolor=#845300
     modified_base   2017..2019
                     /label="V188L"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(6374..6830)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2380..2409
                     /label="myc_epi"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    681..686
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     modified_base   1777..1779
                     /label="modified_base"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     modified_base   1882..1884
                     /label="modified_base"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    663..668
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     modified_base   847..849
                     /label="modified_base"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1312..1329
                     /label="Linker"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     modified_base   832..834
                     /label="K39I"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct
      601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
      721 ttggggatat ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt
      781 ccaggttcca ctggtgacag atctgcgaac gacaccgtag ttgtgggctc gatcaacttt
      841 acagaaggga ttatcgtcgc aaacatggtg gcagagatga ttgaggcgca tacagacctt
      901 aaggtggttc gcaaactgaa ccttggcggg gagaacgtta actttgaagc cattaaacgc
      961 ggaggtgcga ataatggtat tgacatttac gtggagtaca ctgggcacgg tcttgtggat
     1021 attctggggt tcccggagcc gaacgtctat atcaccgccg acaagcagaa gaacggcatc
     1081 aaggcgaact tcaagatccg ccacaacatg gaggacggca gcgtgcagct cgccgaccac
     1141 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1201 agcacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1261 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1321 ggagggagca tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1381 ctggacggcg gcgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     1441 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1501 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1561 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1621 atcagcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
     1681 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1741 gggcacaagc tggagtacaa ctttccgccg cccagctcta ctgatccaga aggtgcatac
     1801 gaaaccgtga agaaggagta caaacgtaaa tggaatattg tatggctcaa accactggga
     1861 ttcaacaata cgtatacgct taccgttaaa gacgaactgg cgaaacagta taaccttaaa
     1921 accttcagtg acttagcgaa aatctcggat aagctgattc tgggtgcaac gatgttcttt
     1981 ttagaagggc ccgatggtta cccaggcctg caaaaactgt acaatttcaa attcaagcac
     2041 accaaaagca tggacatggg tattcgctat accgccattg ataataacga agttcaggta
     2101 attgatgcct gggccactga tggcttgctg gtgagccaca aattaaaaat tctggaggat
     2161 gataaagcgt tcttcccgcc gtattatgct gcccccatca tccgtcagga tgtcttagat
     2221 aagcatcctg aactgaagga cgtgctgaac aaactcgcga atcaaatttc actggaagaa
     2281 atgcagaaac tgaattacaa ggtggacggt gagggtcagg acccagcgaa agtagctaag
     2341 gagtttttga aagagaaagg tttaattctg caggtcgacg aacaaaaact catctcagaa
     2401 gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc acactccttg
     2461 ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac catcatctcc
     2521 cttatcatcc tcatcatgct ttggcagaag aagccacgtt aggcggccgc tcgagatcag
     2581 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2641 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2701 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2761 aggattggga agacaatagc aggcgtgctg gggatgcggt gggctctatg gcttctgagg
     2821 cggaaagaac cagtggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2881 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2941 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3001 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3061 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3121 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3181 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3241 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3301 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3361 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     3421 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3541 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3601 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3661 aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc cgccccgacg
     3721 ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga aaaggaagaa
     3781 acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     3841 gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt
     3901 attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc
     3961 cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     4021 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     4081 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc
     4141 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     4201 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     4261 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     4321 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     4381 cgagatcctc gccgtcgggc ttgctcgcct tgagcctggc gaacagttcg gctggcgcga
     4441 gcccctgatg ctcttgatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     4501 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     4561 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     4621 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     4681 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     4741 cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     4801 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     4861 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     4921 caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa gcctaggcct
     4981 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggaggc ggcctcggcc
     5041 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     5101 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     5161 ttgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     5221 taattgagat gcttgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     5281 cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc ctttttcaat
     5341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6361 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6421 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6601 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6721 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6781 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cg
//