LOCUS Double_UP 6734 bp ds-DNA circular 16-OCT-2019 DEFINITION . FEATURES Location/Qualifiers CDS complement(5746..6405) /label="AmpR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS 2963..3655 /label="mScarlet" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 1736..1769 /label="LoxP" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin complement(4966..5648) /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 3769..4200 /label="rb_glob_pA terminator" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 4..1675 /label="CAG Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 1773..2477 /label="mNeonGreen" /ApEinfo_revcolor=#008040 /ApEinfo_fwdcolor=#008040 misc_feature 2488..2919 /label="rb_glob_pA terminator" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS 3671..3727 /label="P2A" /ApEinfo_revcolor=#008080 /ApEinfo_fwdcolor=#008080 misc_feature 2920..2953 /label="LoxP" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 ORIGIN 1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC 421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA 481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG 541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT 601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC 661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC 721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC 781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG 841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG 901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG 961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC 1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG 1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC 1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTaG CGGGGCGTGG 1201 CGCGGGGCTC GCCGTGCCGG GCGGGGGGTG GCGGCAGGTG GGGGTGCCGG GCGGGGCGGG 1261 GCCGCCTCGG GCCGGGGAGG GCTCGGGGGA GGGGCGCGGC GGcCCCCGGA GCGCCGGCGG 1321 CTGTCGAGGC GCGGCGAGCC GCAGCCATTG CCTTTTATGG TAATCGTGCG AGAGGGCGCA 1381 GGGACTTCCT TTGTCCCAAA TCTGtGCGGA GCCGAAATCT GGGAGGCGCC GCCGCACCCC 1441 CTCTAGCGGG CGCGGGgCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG GCGGGGAGGG 1501 CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC CAGCCTCGGG GCTGTCCGCG 1561 GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC 1621 CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT 1681 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTGC TGCAGATAAC 1741 TTCGTATAAT GTATGCTATA CGAAGTTATA TGGTGAGCAA GGGCGAGGAG GATAACATGG 1801 CCTCTCTCCC AGCGACACAT GAGTTACACA TCTTTGGCTC CATCAACGGT GTGGACTTTG 1861 ACATGGTGGG TCAGGGCACC GGCAATCCAA ATGATGGTTA TGAGGAGTTA AACCTGAAGT 1921 CCACCAAGGG TGACCTCCAG TTCTCCCCCT GGATTCTGGT CCCTCATATC GGGTATGGCT 1981 TCCATCAGTA CCTGCCCTAC CCTGACGGGA TGTCGCCTTT CCAGGCCGCC ATGGTAGATG 2041 GCTCCGGCTA CCAAGTCCAT CGCACAATGC AGTTTGAAGA TGGTGCCTCC CTTACTGTTA 2101 ACTACCGCTA CACCTACGAG GGAAGCCACA TCAAAGGAGA GGCCCAGGTG AAGGGGACTG 2161 GTTTCCCTGC TGACGGTCCT GTGATGACCA ACTCGCTGAC CGCTGCGGAC TGGTGCAGGT 2221 CGAAGAAGAC TTACCCCAAC GACAAAACCA TCATCAGTAC CTTTAAGTGG AGTTACACCA 2281 CTGGAAATGG CAAGCGCTAC CGGAGCACTG CGCGGACCAC CTACACCTTT GCCAAGCCAA 2341 TGGCGGCTAA CTATCTGAAG AACCAGCCGA TGTACGTGTT CCGTAAGACG GAGCTCAAGC 2401 ACTCCAAGAC CGAGCTCAAC TTCAAGGAGT GGCAAAAGGC CTTTACCGAT GTGATGGGCA 2461 TGGACGAGCT GTACAAGTAA AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG 2521 AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG 2581 TGTTGGAATT TTTTGTGTCT CTCACTCGGA AGGACATATG GGAGGGCAAA TCATTTAAAA 2641 CATCAGAATG AGTATTTGGT TTAGAGTTTG GCAACATATG CCCATATGCT GGCTGCCATG 2701 AACAAAGGTT GGCTATAAAG AGGTCATCAG TATATGAAAC AGCCCCCTGC TGTCCATTCC 2761 TTATTCCATA GAAAAGCCTT GACTTGAGGT TAGATTTTTT TTATATTTTG TTTTGTGTTA 2821 TTTTTTTCTT TAACATCCCT AAAATTTTCC TTACATGTTT TACTAGCCAG ATTTTTCCTC 2881 CTCTCCTGAC TACTCCCAGT CATAGCTGTC CCTCTTCTCA TAACTTCGTA TAATGTATGC 2941 TATACGAAGT TATGGTACCA TGgtgagcaa gggcgaggca gtgatcaagg agttcatgcg 3001 gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg 3061 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 3121 cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcat 3181 caagcacccc gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg 3241 ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct 3301 ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg 3361 ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga 3421 ggacggcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggAcgcta 3481 cctggcggac ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta 3541 caacgtcgac cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca 3601 gtacgaacgc tccgagggcc gccactccac cggcggcatg gacgagctgt acaagTTCGA 3661 Aggcagcggc gctactaact tcagcctgct gaagcaggct ggagacgtgg aagagaatcc 3721 tggacctATC GATACCGGTC AATTGGCGGC CGCGCATGCG CTAGCtaaTT TCCCTCTGCC 3781 AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA 3841 TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT 3901 GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT 3961 GCCCATATGC TGGCTGCCAT GAACAAAGGT TGGCTATAAA GAGGTCATCA GTATATGAAA 4021 CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG TTAGATTTTT 4081 TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC CTTACATGTT 4141 TTACTAGCCA GATTTTTCCT CCTCTCCTGA CTACTCCCAG TCATAGCTGT CCCTCTTCTC 4201 TTATGGAGAT CCCTCGACAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA 4261 TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 4321 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA 4381 GTCGGGAAAC CTGTCGTGCC AGCGGATCCG CATCTCAATT AGTCAGCAAC CATAGTCCCG 4441 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 4501 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 4561 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT AACTTGTTTA 4621 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 4681 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 4741 GGATCCGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 4801 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 4861 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 4921 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 4981 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 5041 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 5101 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 5161 AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 5221 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 5281 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 5341 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 5401 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 5461 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 5521 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 5581 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 5641 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 5701 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 5761 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 5821 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 5881 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 5941 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 6001 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 6061 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 6121 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 6181 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 6241 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 6301 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 6361 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 6421 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 6481 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 6541 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 6601 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 6661 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 6721 AAAAGTGCCA CCTG //