LOCUS       Exported                8120 bp ds-DNA     circular SYN 25-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 25, 2019 from SnapGene 4.3.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    263..826
                     /label=pU6 (pMJ177) 5' CMV promoter IE
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    complement(766..775)
                     /label=GC Box
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    844..1024
                     /label=HIV-1_5_LTR
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    1541..1542
                     /label=deletion of cg here
                     /note="/ApEinfo_fwdcolor=#f58a5e
                     /ApEinfo_revcolor=#f58a5e"
     misc_feature    complement(1599..1605)
                     /label=TATA box
                     /note="/ApEinfo_fwdcolor=#ff9ccd
                     /ApEinfo_revcolor=#ff9ccd"
     misc_feature    1693..1926
                     /label=RRE
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     misc_feature    2457..2471
                     /label=cPPT
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    2692..3653
                     /label=EF1Alpha
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    3655..3809
                     /label=EF1Alpha(1)
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    3875..3877
                     /label=start
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     CDS             3878..4582
                     /codon_start=1
                     /label=mCherry
                     /note="/ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    4583..4639
                     /label=P2A
                     /note="/ApEinfo_fwdcolor=#faac61
                     /ApEinfo_revcolor=#faac61"
     CDS             4640..4900
                     /codon_start=1
                     /label=LmoAcrIIA4
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
                     /translation="NINDLIREIKNKDYTVKLSGTDSNSITQLIIRVNNDGNEYVISES
                     ENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELN"
     misc_recomb     4920..4953
                     /label=LoxP recombination site(1)
                     /note="/ApEinfo_fwdcolor=#4682b4
                     /ApEinfo_revcolor=#4682b4"
     misc_feature    4999..5599
                     /label=wPRE
                     /note="/ApEinfo_fwdcolor=#0060ff
                     /ApEinfo_revcolor=#0060ff"
     misc_feature    5789..5803
                     /label=cPPT(1)
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    6126..6306
                     /label=HIV-1_5_LTR(1)
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     rep_origin      complement(6350..7032)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="/ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080"
     misc_feature    complement(6353..6972)
                     /label=pBR322_origin
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     misc_feature    complement(6377..6386)
                     /label=GC Box(1)
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     CDS             complement(7130..7789)
                     /label=AmpR
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    complement(8029..8057)
                     /label=AmpR promoter
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     1561 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1621 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1681 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1741 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1801 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1861 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1921 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1981 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     2041 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2101 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2161 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2221 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2281 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2341 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2401 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2461 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2521 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2581 acagggacag cagagatcca gtttggttag taccgggccc gctctagaga tagtctcgag
     2641 tactaggatc cattaggcgg ccgcgtggat aaccgtatta ccgccatgca tgtgcccgtc
     2701 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2761 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2821 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2881 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2941 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     3001 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     3061 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     3121 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     3181 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     3241 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     3301 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     3361 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     3421 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     3481 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3541 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3601 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgcgctttt
     3661 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3721 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3781 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3841 ttcttccatt tcaggtgtcg tgagctagcc caccatggtg agcaaaggcg aggaggataa
     3901 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     3961 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     4021 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     4081 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     4141 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4201 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4261 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4321 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4381 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     4441 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     4501 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     4561 cggcatggac gagctgtaca aggctactaa cttctccctg ctgaaacagg caggagacgt
     4621 ggaggagaac cctggaccaa acatcaacga cctgatccgg gagatcaaga acaaggacta
     4681 caccgtgaag ctgagcggca ccgatagcaa cagcatcacc cagctgatta tccgcgtgaa
     4741 caacgacggc aacgagtacg tgatctccga gagcgagaac gagagcatcg tggagaagtt
     4801 catcagcgcc ttcaagaacg gttggaacca ggagtacgag gacgaggagg agttctacaa
     4861 cgacatgcag accatcaccc tgaagagcga gctgaactaa gaattcgtcg agggacctaa
     4921 taacttcgta tagcatacat tatacgaagt tatacatgtt taagggttcc ggttccacta
     4981 ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     5041 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5101 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5161 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5221 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5281 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5341 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5401 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5461 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5521 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5581 ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag
     5641 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     5701 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     5761 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5821 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5881 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     5941 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     6001 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     6061 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     6121 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6181 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6241 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6301 ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6781 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7141 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7201 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7261 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7321 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7381 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7441 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7501 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7561 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7621 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7681 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7741 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7801 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7861 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7921 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7981 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8041 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8101 cccgaaaagt gccacctgac
//
