LOCUS       Exported                5856 bp ds-DNA     circular SYN 25-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 25, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pCAP054*_pGL3_dpse_peak47(#22) 
                     IF_mhcI_tGFP_CapSeq_cleaned"
     rep_origin      1..456
                     /label=f1 origin
                     /note="/dnas_title=f1 origin"
                     /note="/vntifkey=33"
     polyA_signal    587..740
                     /label=Synthetic (upstream) poly (A)signal
                     /note="/dnas_title=Synthetic (upstream) poly (A)signal"
                     /note="/vntifkey=25"
     primer_bind     689..708
                     /label=RVprimer3 binding region
                     /note="/dnas_title=RVprimer3 binding region"
                     /note="/vntifkey=28"
     misc_feature    748..752
                     /label=MCS
                     /note="/dnas_title=MCS"
                     /note="/vntifkey=21"
     enhancer        754..1675
                     /note="dpse_peak47#22"
     primer_bind     754..779
                     /label=Primer 1
     primer_bind     complement(1651..1675)
                     /label=Primer 2
     misc_feature    1701..1705
     enhancer        1706..1720
                     /note="homology arm IF"
     gene            1835..2494
                     /label=CamR
                     /note="CamR"
     misc_feature    2836..3141
                     /label=ccdB survival
                     /note="ccdB survival"
     enhancer        3183..3197
                     /note="homology arm IF"
     misc_feature    3234..3245
     misc_feature    3241..3311
                     /label=MHC intron 16
                     /note="MHC intron 16"
     misc_feature    3244..3310
                     /label=intron
                     /note="intron"
     misc_feature    3313..3315
                     /label=insert
                     /note="insert"
     misc_feature    3315
                     /label=HindIII
                     /note="HindIII"
     misc_feature    3315
                     /label=AflII
                     /note="AflII"
     misc_feature    3322..3510
                     /label=sgGFP
                     /note="sgGFP"
     misc_feature    complement(3531..3551)
                     /label=nonstandard type: PCR_primer
                     /note="junction_rev"
                     /note="/current=0"
     polyA_signal    3541..3762
                     /label=SV40 late poly (A) signal
                     /note="/dnas_title=SV40 late poly (A) signal"
                     /note="/vntifkey=25"
     misc_feature    complement(3541..3566)
                     /label=nonstandard type: PCR_primer
                     /note="GSP1"
                     /note="/current=0"
     primer_bind     3844..3865
                     /label=RVprimer4 binding site
                     /note="/dnas_title=RVprimer4 binding site"
                     /note="/vntifkey=28"
     rep_origin      4090..4710
                     /label=pBR322 ori_rep
                     /note="pBR322 ori_rep"
     rep_origin      4103
                     /label=ColE1-derived plasmid replication origin
                     /note="/dnas_title=ColE1-derived plasmid replication 
                     origin"
                     /note="/vntifkey=33"
     misc_feature    complement(4865..5725)
                     /label=Beta-lactamase gene (Ampr)
                     /label=nonstandard type: misc_marker
                     /note="/dnas_title=Beta-lactamase gene (Ampr)"
                     /note="/vntifkey=22"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt accctactgg tgtgacaatt taaggtgagt
      781 gcagtgcgcg ttggggtggg gaaaagtgcc tagtcatcgg aaatctatct aggcgagaac
      841 agcagcagtt cttaacccaa tggcctctcc ccgtttgttc attgtttgcg agccataagt
      901 gttcctttcc ccaacgataa gacaatcaat ctgcaatcac cgcaatagta ttcgacgcga
      961 ctgtcgtcgc cattcccgat aacctttgtg gcgactgctc tagttcctcc gtcctattct
     1021 tgttttgcag ttatgcctcc ctctctctct ctctctctgt gttctccagt gcgctttgtt
     1081 ttcgacgctc ttgccgttaa tcgaattaac aatccgacgc ctgcagctga acaaagttaa
     1141 tcagtctctg cttcatcaac cgccccacta tttgcatgac taagccagag ttcatgcgat
     1201 ctggggagca tttgacagcc gatctgttct gctctgctcg ttatcggcat aactatgtat
     1261 atgactgaac ctgactaatt ccaaatgcat gtgtcacata cggtacgggt gtagttttta
     1321 caaacggggt tccttatcgt tggacttatc gctaggttta ccatatagta ccataaccga
     1381 ttcctttgtt ctttgattga tttattttta aacacctctc gaggaaaatc cagcatttct
     1441 cttaaccgtt ctttgtttga caaaaaaacg tcgccattgc tgaaaatatt aaaaacaata
     1501 tcacatacac cacaaatgaa caaccatata atatttgtgt agatgtaccc agatatacaa
     1561 cccatacata catagccaca cacatatgta ttcaagacgt ttccgtgctt atcttttgtt
     1621 gtttttcctc cccccccggt cccactctcc ctagacgtac gtgtatttct gtctgaattc
     1681 ccatcagacg tctggctcga gatcttagag catgcaccgg tgatatccgg ccgcattagg
     1741 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1801 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1861 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1921 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1981 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2041 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2101 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2161 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2221 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2281 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2341 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2401 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2461 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc
     2521 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2581 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2641 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2701 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2761 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2821 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2881 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     2941 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3001 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     3061 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     3121 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggatat
     3181 cgtcgacgaa ttcggcccct gcaggggcat tccggtactg ttggtaaagc caccatggaa
     3241 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
     3301 tttacttcag gccacttaag catggctagc aaaggagaag aactcttcac tggagttgtc
     3361 ccaattcttg ttgaattaga tggtgatgtt aacggccaca agttctctgt cagtggagag
     3421 ggtgaaggtg atgcaacata cggaaaactt accctgaagt tcatctgcac tactggcaaa
     3481 ctgcctgttc cctggccaac actagtctga tctagagtcg gggcggccgg ccgcttcgag
     3541 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3601 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3661 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     3721 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccga
     3781 ccgatgccct caagctttga gaggggcccc cttcaaccca gtcagctccg acgtcttggc
     3841 gcccccgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     3901 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4861 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4921 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4981 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5041 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5101 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5161 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5221 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5281 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5341 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5401 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5461 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5521 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5581 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5641 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5701 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5761 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5821 gggttccgcg cacatttccc cgaaaagtgc cacctg
//
