LOCUS       TEV_SH        6536 bp    DNA     circular     27-MAR-2019
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12744963|
COMMENT     VNTDBDATE|-12744976|
COMMENT     LSOWNER|
COMMENT     VNTNAME|TEV_SH|
COMMENT     VNTAUTHORNAME|Martin Moche|
COMMENT     VNTAUTHORTEL|0852486843|
COMMENT     VNTAUTHOREML|Martin.Moche@ki.se|
COMMENT     VNTOAUTHORNAME|Susanne van den Berg|
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FEATURES             Location/Qualifiers
     misc_feature    905..946
                     /vntifkey="21"
                     /label=V5\epitope
     misc_feature    956..973
                     /vntifkey="21"
                     /label=6XHis
     misc_recomb     2521..3194
                     /vntifkey="86"
                     /label=pBR322\ori
     misc_feature    complement(5068..6150)
                     /vntifkey="21"
                     /label=lacI
     misc_feature    complement(3565..3756)
                     /vntifkey="21"
                     /label=ROP
     misc_feature    1516..2376
                     /vntifkey="21"
                     /label=amp(R)
     misc_feature    1044..1091
                     /vntifkey="21"
                     /label=T7\term
     misc_feature    89..91
                     /vntifkey="21"
                     /label=ATG
     misc_feature    89..976
                     /vntifkey="21"
                     /label=TEVSH
     promoter        1..17
                     /vntifkey="30"
                     /label=T7
     misc_feature    20..44
                     /vntifkey="21"
                     /label=Lac\operator
BASE COUNT     1586 a      1682 c      1737 g      1531 t 
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt 
       61 tgtttaactt taagaaggag atatacatat gcgttcatca acaagtttgt acaaaaaagc 
      121 aggctcggga gaaagcttgt ttaagggacc acgtgattat aacccgatat cgagctccat 
      181 ttgtcatttg acgaatgaat ctgatgggca cacaacatcg ttgtatggta ttggatttgg 
      241 tcccttcatc attacaaaca agcacttgtt tagaagaaat aatggaacac tgttggtcca 
      301 atcactacat ggtgtattca aggtcaagga caccacgact ttgcaacaac acctcgttga 
      361 tgggagggac atgataatta ttcgcatgcc taaggatttc ccaccatttc ctcaaaagct 
      421 gaaatttaga gagccacaaa gggaagagcg catatgtctt gtgacaacca acttccaaac 
      481 taagagtatg tctagcatgg tgtcagacac tagttgcaca ttcccttcat ctgatggcat 
      541 attctggaag cattggattc aaaccaagga tgggcagtgt ggcagtccat tagtatcaac 
      601 tagagatggg ttcattgttg gtatacactc agcatcgaat ttcaccaaca caaacaatta 
      661 tttcacaagc gtgccgaaaa acttcatgga attgttgaca aatcaggagg cgcagcagtg 
      721 ggttagtggt tggcgattaa atgctgactc agtattgtgg gggggccata aagttttcat 
      781 gaacaaacct gaagagcctt ttcagccagt taaggaagcg actcaactca tgaatgaatt 
      841 ggtgtactcg caagacccag ctttcttgta caaagtggtt gatctggagg gcccgcggtt 
      901 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca 
      961 tcaccatcac cattgagttt gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg 
     1021 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga 
     1081 ggggtttttt gctgaaagga ggaactatat ccggatatcc cgcaagaggc ccggcagtac 
     1141 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag 
     1201 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact 
     1261 accgcattaa agcttatcga tgataagctg tcaaacatga gaattaattc ttgaagacga 
     1321 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 
     1381 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 
     1441 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 
     1501 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 
     1561 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 
     1621 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 
     1681 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 
     1741 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat 
     1801 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 
     1861 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 
     1921 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 
     1981 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 
     2041 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa 
     2101 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 
     2161 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 
     2221 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 
     2281 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 
     2341 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 
     2401 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 
     2461 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 
     2521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 
     2581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 
     2641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 
     2701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 
     2761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 
     2821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 
     2881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 
     2941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 
     3001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 
     3061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 
     3121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 
     3181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 
     3241 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 
     3301 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 
     3361 tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 
     3421 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac 
     3481 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 
     3541 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 
     3601 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc 
     3661 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg 
     3721 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt 
     3781 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac 
     3841 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat 
     3901 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt 
     3961 aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca 
     4021 gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt 
     4081 tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg 
     4141 tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag 
     4201 gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg 
     4261 gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac 
     4321 agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg 
     4381 ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg 
     4441 aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc 
     4501 gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga 
     4561 gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg 
     4621 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc 
     4681 atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg 
     4741 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga 
     4801 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag 
     4861 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc 
     4921 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag 
     4981 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg 
     5041 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     5101 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     5161 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg 
     5221 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg 
     5281 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac 
     5341 taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag 
     5401 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg 
     5461 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg 
     5521 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga 
     5581 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac 
     5641 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga 
     5701 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg 
     5761 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac 
     5821 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc 
     5881 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag 
     5941 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg 
     6001 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga 
     6061 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc 
     6121 tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg 
     6181 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac 
     6241 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga 
     6301 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca 
     6361 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc 
     6421 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg 
     6481 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat 
//
