LOCUS Exported 4519 bp ds-DNA circular SYN 26-MAR-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4519) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Mar 27, 2019 from SnapGene Viewer 4.3.4 https://www.snapgene.com FEATURES Location/Qualifiers source 1..4519 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(236..864) /direction=LEFT /label=ColE1 origin CDS complement(1016..1675) /label=AmpR promoter complement(1915..1943) /label=Amp prom rep_origin 2014..2454 /direction=RIGHT /label=F1 ori CDS complement(2461..2529) /label=LacZ alpha primer_bind 2599..2617 /label=M13-fwd primer_bind 2627..2646 /label=T7 misc_feature 2681..2750 /label=attB misc_feature 2778..2877 /label=SA misc_feature 2878 /label=Ph2-5' CDS 2879..2917 /codon_start=1 /label=(GGS)4-BHI linker Phase2 /translation="SGGSGGSGGSGGS" CDS 2918..2971 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 2972..3709 /codon_start=1 /gene="lexA" /product="E. coli DNA-binding protein that represses genes involved in the SOS response to DNA damage" /label=LexA /note="The N-terminal DNA-binding domain recognizes the 16-bp palindromic sequence 5'-CTGTATATATATACAG-3'." /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWLGSPADALDDFDLDMLPADAL DDFDLDMLPADALDDFDLDMLPG" 3'UTR 3710..4036 /label=hsp70 3'UTR CDS 4043..4072 /codon_start=1 /label=(GGS)4 linker Phase2 /translation="GGSGGSGGSG" misc_feature 4073..4074 /label=Ph2-3' misc_feature 4075..4195 /label=SD misc_feature complement(4223..4292) /label=attB primer_bind complement(4330..4349) /label=T3 primer_bind complement(4367..4385) /label=M13-rev misc_binding complement(4393..4415) /label=LacO promoter complement(4420..4449) /label=lac ORIGIN 1 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 61 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 661 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 1021 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 1081 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 1141 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 1201 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 1261 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 1321 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 1381 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 1441 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 1501 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 1561 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 1621 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 1681 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 1741 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 1801 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 1861 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 1921 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 1981 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt 2041 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 2101 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 2161 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 2221 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 2281 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 2341 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 2401 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 2461 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 2521 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 2581 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact 2641 atagggcgaa ttggagctcg ctagcgatgt aggtcacggt ctcgaagccg cggtgcgggt 2701 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca 2761 tgatggtcga ctctagaagt cgatccaaca tggcgacttg tcccatcccc ggcatgttta 2821 aatatactaa ttattcttga actaatttta atcaaccgat ttatctctct tccgcaggtc 2881 gggaggttcc ggtggaagcg gaggtagcgg cggatccgaa ggacgcggaa gcctgttgac 2941 gtgcggagat gtggaagaga atccaggacc gatgaaagcg ttaacggcca ggcaacaaga 3001 ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga 3061 aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct 3121 ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca 3181 ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cacttctggc 3241 gcaacagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc cgaatgctga 3301 tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg atggtgactt 3361 gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg cacgtattga 3421 tgacgaggtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac tgttgccaga 3481 aaatagcgag tttaaaccaa ttgtcgtaga tcttcgtcag cagagcttca ccattgaagg 3541 gctggcggtt ggggttattc gcaacggcga ctggctggga tctccggccg acgccctgga 3601 cgacttcgac ctggacatgc tgccggccga cgccctggac gacttcgacc tggacatgct 3661 gccggccgac gccctggacg acttcgacct ggacatgctg ccggggtaac taagtaagga 3721 tctagacatg ataagataca ttgatgagtt tggacaaagc ttatcgatac cgtcgactaa 3781 agccaaatag aaaattattc agttcctggc ttaagttttt aaaagtgata ttatttattt 3841 ggttgtaacc aaccaaaaga atgtaaataa ctaatacata attatgttag ttttaagtta 3901 gcaacaaatt gattttagct atattagcta cttggttaat aaatagaata tatttattta 3961 aagataattg cgtttttatt gtcagggagt gagtttgctt aaaaactcgt ttagatccac 4021 tagttctaga gcggccggat ccggaggtag cggtggaagc ggaggttccg gcaggtaagt 4081 tattgaacaa tggcatcaaa tgccttcatc atcactaccc tttagccctt aagaccccac 4141 aatgacctta cccactcaga gaaaaaagta aatatgaaag cccatttgaa cttctaagct 4201 tgtcgaccat catgatggac cagatgggtg aggtggagta cgcgcccggg gagcccaagg 4261 gcacgccctg gcacccgcac cgcggcttcg agaccgtgac ctacatcatg catggtaccc 4321 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg 4381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 4441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 4501 ctgcccgctt tccagtcgg //