LOCUS Exported 6100 bp ds-DNA circular SYN 23-MAR-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6100) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Mar 28, 2019 from SnapGene Viewer 4.3.4 https://www.snapgene.com FEATURES Location/Qualifiers source 1..6100 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..130 /label=ITR misc_feature 151..708 /label=CMV promoter misc_feature 753..769 /label=T7 promoter primer misc_feature 779..784 /label=HindIII misc_feature 794..820 /label=HA tag misc_feature 821..2056 /label=beta2-AR misc_feature 863..865 /label=G>R misc_feature 896..898 /label=E>Q misc_feature 897..898 /label=Mutation misc_feature 2057..2092 /label=NNES misc_feature 2099..2104 /label=NheI misc_feature 2105..2614 /label=Nanoluc misc_feature 2615..2659 /label=15 aa linker misc_feature 2660..3091 /label=LOV2 (LOVTRAP) misc_feature 2696 /label=V416L misc_feature 3092..3097 /label=EcoRI misc_feature 3104..3335 /label=PolyA misc_feature 3364..3504 /label=ITR /label=ITR(1) ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgttgtaca tagtaatcaa ttacggggtc attagttcat 181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 301 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta 361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact 721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa 781 gcttgccacc atgtatccgt acgacgtacc agactacgca gggcaacccg ggaacggcag 841 cgccttcttg ctggcaccca atagaagcca tgcgccggac cacgacgtca cgcagcagag 901 ggacgaggtg tgggtggtgg gcatgggcat cgtcatgtct ctcatcgtcc tggccatcgt 961 gtttggcaat gtgctggtca tcacagccat tgccaagttc gagcgtctgc agacggtcac 1021 caactacttc atcacttcac tggcctgtgc tgatctggtc atgggcctgg cagtggtgcc 1081 ctttggggcc gcccatattc ttatgaaaat gtggactttt ggcaacttct ggtgcgagtt 1141 ttggacttcc attgatgtgc tgtgcgtcac ggccagcatt gagaccctgt gcgtgatcgc 1201 agtggatcgc tactttgcca ttacttcacc tttcaagtac cagagcctgc tgaccaagaa 1261 taaggcccgg gtgatcattc tgatggtgtg gattgtgtca ggccttacct ccttcttgcc 1321 cattcagatg cactggtacc gggccaccca ccaggaagcc atcaactgct atgccaatga 1381 gacctgctgt gacttcttca cgaaccaagc ctatgccatt gcctcttcca tcgtgtcctt 1441 ctacgttccc ctggtgatca tggtcttcgt ctactccagg gtctttcagg aggccaaaag 1501 gcagctccag aagattgaca aatctgaggg ccgcttccat gtccagaacc ttagccaggt 1561 ggagcaggat gggcggacgg ggcatggact ccgcagatct tccaagttct gcttgaagga 1621 gcacaaagcc ctcaagacgt taggcatcat catgggcact ttcaccctct gctggctgcc 1681 cttcttcatc gttaacattg tgcatgtgat ccaggataac ctcatccgta aggaagttta 1741 catcctccta aattggatag gctatgtcaa ttctggtttc aatcccctta tctactgccg 1801 gagcccagat ttcaggattg ccttccagga gcttctgtgc ctgcgcaggt cttctttgaa 1861 ggcctatggg aatggctact ccagcaacgg caacacaggg gagcagagtg gatatcacgt 1921 ggaacaggag aaagaaaata aactgctgtg tgaagacctc ccaggcacgg aagactttgt 1981 gggccatcaa ggtactgtgc ctagcgataa cattgattca caagggagga attgtagtac 2041 aaatgactca ctgctggagc tggccgagaa gctggccggc ctggacatca acggtggagc 2101 tagcgtcttc acactcgaag atttcgttgg ggactggcga cagacagccg gctacaacct 2161 ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt 2221 aactccgatc caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt 2281 catcatcccg tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa 2341 ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg gcacactggt 2401 aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg aaggcatcgc 2461 cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca acaaaattat 2521 cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca tcaacggagt 2581 gaccggctgg cggctgtgcg aacgcattct ggcgggcgac ccactagtcc agtgtggtgg 2641 aattgccgga tccgccacct tggctactac acttgaacgt attgagaaga actttctcat 2701 tactgaccca agattgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt 2761 gacagaatat agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga 2821 aactgatcgc gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac 2881 tgttcagctg attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca 2941 gcctatgcga gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac 3001 tgagcatgtc cgagatgctg ccgagagaga gggagtcatg ctgattaaga aaactgcaga 3061 aaatattgat gaggcggcaa aagaacttta agaattcctc gagctagagc tcgctgatca 3121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 3181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 3241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 3301 gaggattggg aagacaatag caggcatgct ggggaggtaa ccacgtgcgg accgagcggc 3361 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg 3421 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg 3481 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt 3541 gcggtattca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta 3601 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3661 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 3721 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 3781 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt 3841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 3901 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc 3961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 4021 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 4081 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 4141 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 4201 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat 4261 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 4321 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 4381 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 4441 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 4501 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 4561 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 4621 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 4681 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 4741 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 4801 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 4861 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 4921 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 4981 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 5041 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 5101 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg 5161 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 5221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 5281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 5341 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 5401 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 5461 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 5521 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 5581 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 5641 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 5701 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 5761 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 5821 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5881 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 6001 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 6061 ttacggttcc tggccttttg ctggcctttt gctcacatgt //