LOCUS       Exported                6100 bp ds-DNA     circular SYN 23-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 28, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /label=ITR
     misc_feature    151..708
                     /label=CMV promoter
     misc_feature    753..769
                     /label=T7 promoter primer
     misc_feature    779..784
                     /label=HindIII
     misc_feature    794..820
                     /label=HA tag
     misc_feature    821..2056
                     /label=beta2-AR
     misc_feature    863..865
                     /label=G>R
     misc_feature    896..898
                     /label=E>Q
     misc_feature    897..898
                     /label=Mutation
     misc_feature    2057..2092
                     /label=NNES
     misc_feature    2099..2104
                     /label=NheI
     misc_feature    2105..2614
                     /label=Nanoluc
     misc_feature    2615..2659
                     /label=15 aa linker
     misc_feature    2660..3091
                     /label=LOV2 (LOVTRAP)
     misc_feature    2696
                     /label=V416L
     misc_feature    3092..3097
                     /label=EcoRI
     misc_feature    3104..3335
                     /label=PolyA
     misc_feature    3364..3504
                     /label=ITR
                     /label=ITR(1)
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca tagtaatcaa ttacggggtc attagttcat
      181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      301 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
      361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      781 gcttgccacc atgtatccgt acgacgtacc agactacgca gggcaacccg ggaacggcag
      841 cgccttcttg ctggcaccca atagaagcca tgcgccggac cacgacgtca cgcagcagag
      901 ggacgaggtg tgggtggtgg gcatgggcat cgtcatgtct ctcatcgtcc tggccatcgt
      961 gtttggcaat gtgctggtca tcacagccat tgccaagttc gagcgtctgc agacggtcac
     1021 caactacttc atcacttcac tggcctgtgc tgatctggtc atgggcctgg cagtggtgcc
     1081 ctttggggcc gcccatattc ttatgaaaat gtggactttt ggcaacttct ggtgcgagtt
     1141 ttggacttcc attgatgtgc tgtgcgtcac ggccagcatt gagaccctgt gcgtgatcgc
     1201 agtggatcgc tactttgcca ttacttcacc tttcaagtac cagagcctgc tgaccaagaa
     1261 taaggcccgg gtgatcattc tgatggtgtg gattgtgtca ggccttacct ccttcttgcc
     1321 cattcagatg cactggtacc gggccaccca ccaggaagcc atcaactgct atgccaatga
     1381 gacctgctgt gacttcttca cgaaccaagc ctatgccatt gcctcttcca tcgtgtcctt
     1441 ctacgttccc ctggtgatca tggtcttcgt ctactccagg gtctttcagg aggccaaaag
     1501 gcagctccag aagattgaca aatctgaggg ccgcttccat gtccagaacc ttagccaggt
     1561 ggagcaggat gggcggacgg ggcatggact ccgcagatct tccaagttct gcttgaagga
     1621 gcacaaagcc ctcaagacgt taggcatcat catgggcact ttcaccctct gctggctgcc
     1681 cttcttcatc gttaacattg tgcatgtgat ccaggataac ctcatccgta aggaagttta
     1741 catcctccta aattggatag gctatgtcaa ttctggtttc aatcccctta tctactgccg
     1801 gagcccagat ttcaggattg ccttccagga gcttctgtgc ctgcgcaggt cttctttgaa
     1861 ggcctatggg aatggctact ccagcaacgg caacacaggg gagcagagtg gatatcacgt
     1921 ggaacaggag aaagaaaata aactgctgtg tgaagacctc ccaggcacgg aagactttgt
     1981 gggccatcaa ggtactgtgc ctagcgataa cattgattca caagggagga attgtagtac
     2041 aaatgactca ctgctggagc tggccgagaa gctggccggc ctggacatca acggtggagc
     2101 tagcgtcttc acactcgaag atttcgttgg ggactggcga cagacagccg gctacaacct
     2161 ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt
     2221 aactccgatc caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt
     2281 catcatcccg tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa
     2341 ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg gcacactggt
     2401 aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg aaggcatcgc
     2461 cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca acaaaattat
     2521 cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca tcaacggagt
     2581 gaccggctgg cggctgtgcg aacgcattct ggcgggcgac ccactagtcc agtgtggtgg
     2641 aattgccgga tccgccacct tggctactac acttgaacgt attgagaaga actttctcat
     2701 tactgaccca agattgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt
     2761 gacagaatat agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga
     2821 aactgatcgc gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac
     2881 tgttcagctg attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca
     2941 gcctatgcga gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac
     3001 tgagcatgtc cgagatgctg ccgagagaga gggagtcatg ctgattaaga aaactgcaga
     3061 aaatattgat gaggcggcaa aagaacttta agaattcctc gagctagagc tcgctgatca
     3121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3301 gaggattggg aagacaatag caggcatgct ggggaggtaa ccacgtgcgg accgagcggc
     3361 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3421 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3481 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3541 gcggtattca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     3601 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3661 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3721 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3781 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3901 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     3961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4021 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4081 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4141 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     4201 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4261 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4321 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4381 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4441 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4501 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4561 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4621 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4681 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4741 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4801 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4861 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4921 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4981 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5041 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5101 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
     5161 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5341 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5401 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5461 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5521 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5581 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5641 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5701 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5761 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5821 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5881 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6001 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6061 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
