LOCUS       Exported                6103 bp ds-DNA     circular SYN 23-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 28, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /label=ITR
     misc_feature    151..708
                     /label=CMV promoter
     misc_feature    753..769
                     /label=T7 promoter primer
     misc_feature    779..784
                     /label=HindIII
     misc_feature    791..841
                     /label=gLuc signal peptide
     misc_feature    842..1351
                     /label=Nanoluc
     misc_feature    1352..1378
                     /label=HA tag
     misc_feature    1379..2614
                     /label=beta2-AR
     misc_feature    1421..1423
                     /label=G>R
     misc_feature    1454..1456
                     /label=E>Q
     misc_feature    1455..1456
                     /label=Mutation
     misc_feature    2615..2650
                     /label=NNES
     misc_feature    2657..2662
                     /label=NheI
     misc_feature    2663..3094
                     /label=LOV2 (LOVTRAP)
     misc_feature    2699..2701
                     /label=V416L
     misc_feature    3095..3100
                     /label=EcoRI
     misc_feature    3107..3338
                     /label=PolyA
     misc_feature    3367..3507
                     /label=ITR
                     /label=ITR(1)
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca tagtaatcaa ttacggggtc attagttcat
      181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      301 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
      361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      781 gcttgccacc atgggagtca aagttctgtt tgccctgatc tgcatcgctg tggccgaggc
      841 cgtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga
      901 ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac
      961 tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat
     1021 catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt
     1081 ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat
     1141 cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt
     1201 gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga
     1261 cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac
     1321 cggctggcgg ctgtgcgaac gcattctggc gtatccgtac gacgtaccag actacgcagg
     1381 gcaacccggg aacggcagcg ccttcttgct ggcacccaat agaagccatg cgccggacca
     1441 cgacgtcacg cagcagaggg acgaggtgtg ggtggtgggc atgggcatcg tcatgtctct
     1501 catcgtcctg gccatcgtgt ttggcaatgt gctggtcatc acagccattg ccaagttcga
     1561 gcgtctgcag acggtcacca actacttcat cacttcactg gcctgtgctg atctggtcat
     1621 gggcctggca gtggtgccct ttggggccgc ccatattctt atgaaaatgt ggacttttgg
     1681 caacttctgg tgcgagtttt ggacttccat tgatgtgctg tgcgtcacgg ccagcattga
     1741 gaccctgtgc gtgatcgcag tggatcgcta ctttgccatt acttcacctt tcaagtacca
     1801 gagcctgctg accaagaata aggcccgggt gatcattctg atggtgtgga ttgtgtcagg
     1861 ccttacctcc ttcttgccca ttcagatgca ctggtaccgg gccacccacc aggaagccat
     1921 caactgctat gccaatgaga cctgctgtga cttcttcacg aaccaagcct atgccattgc
     1981 ctcttccatc gtgtccttct acgttcccct ggtgatcatg gtcttcgtct actccagggt
     2041 ctttcaggag gccaaaaggc agctccagaa gattgacaaa tctgagggcc gcttccatgt
     2101 ccagaacctt agccaggtgg agcaggatgg gcggacgggg catggactcc gcagatcttc
     2161 caagttctgc ttgaaggagc acaaagccct caagacgtta ggcatcatca tgggcacttt
     2221 caccctctgc tggctgccct tcttcatcgt taacattgtg catgtgatcc aggataacct
     2281 catccgtaag gaagtttaca tcctcctaaa ttggataggc tatgtcaatt ctggtttcaa
     2341 tccccttatc tactgccgga gcccagattt caggattgcc ttccaggagc ttctgtgcct
     2401 gcgcaggtct tctttgaagg cctatgggaa tggctactcc agcaacggca acacagggga
     2461 gcagagtgga tatcacgtgg aacaggagaa agaaaataaa ctgctgtgtg aagacctccc
     2521 aggcacggaa gactttgtgg gccatcaagg tactgtgcct agcgataaca ttgattcaca
     2581 agggaggaat tgtagtacaa atgactcact gctggagctg gccgagaagc tggccggcct
     2641 ggacatcaac ggtggagcta gcttggctac tacacttgaa cgtattgaga agaactttct
     2701 cattactgac ccaagattgc cagataatcc cattatattc gcgtccgata gtttcttgca
     2761 gttgacagaa tatagccgtg aagaaatttt gggaagaaac tgcaggtttc tacaaggtcc
     2821 tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc atagataacc aaacagaggt
     2881 cactgttcag ctgattaatt atacaaagag tggtaaaaag ttctggaacc tctttcactt
     2941 gcagcctatg cgagatcaga agggagatgt ccagtacttt attggggttc agttggatgg
     3001 aactgagcat gtccgagatg ctgccgagag agagggagtc atgctgatta agaaaactgc
     3061 agaaaatatt gatgaggcgg caaaagaact ttaagaattc ctcgagctag agctcgctga
     3121 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3181 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3241 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3301 ggggaggatt gggaagacaa tagcaggcat gctggggagg taaccacgtg cggaccgagc
     3361 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     3421 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     3481 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     3541 tgtgcggtat tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     3601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3781 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3841 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3901 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     3961 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4021 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4081 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     4141 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     4201 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4261 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4321 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4381 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4441 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4501 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4561 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4621 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4681 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4741 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4801 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4861 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4921 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4981 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5041 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5101 tggtttattg ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca
     5161 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5221 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5281 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5581 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
