LOCUS P7_AAVCMV_NanoLu 6328 bp ds-DNA circular 23-MAR-2019 SOURCE ORGANISM COMMENT NewMCS:XbaI,BamHI,NheI,AgeI,BstBI,AfeI(blunt),HpaI(blunt),AscI(8cut ter),SbfI,EcoRIOtherpossibleuniquecutters(currentlynosites)BsiWI,Bm tI(samesequenceasNheI)Tochangepromoter cutwith PacIand XbaI COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 3592..3732 /label=ITR /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 753..769 /label=T7 promoter primer /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 785..786 /label=NotI /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="#ff8040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3332..3563 /label=PolyA /ApEinfo_fwdcolor="#ff2549" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 794..1303 /label=Nanoluc /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 151..708 /label=CMV promoter /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1304..1348 /label=15 aa linker /ApEinfo_fwdcolor="#ff80ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3296..3313 /label=S219V-tev in BL21 DNA /ApEinfo_fwdcolor="#a486ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 791..793 /label=Kozak consensus /ApEinfo_fwdcolor="#ffd92b" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 791..793 /label=start codon /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(2843..3294,3296..3313) /label=TEV for yeast /ApEinfo_fwdcolor="#ff51d8" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1..130 /label=ITR(1) /ApEinfo_label="ITR" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2660..2842 /label=S219V-tev in BL21 DNA(1) /ApEinfo_label="S219V-tev in BL21 DNA" /ApEinfo_fwdcolor="#a486ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1349..2575 /label=rat beta arrestin 2 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3320..3325 /label=EcoRI /ApEinfo_fwdcolor="#46ff6e" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2843..3294 /label=S219V-tev in BL21 DNA(2) /ApEinfo_label="S219V-tev in BL21 DNA" /ApEinfo_fwdcolor="#a486ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(2660..2803,2810..2842) /label=TEV for yeast(1) /ApEinfo_label="TEV for yeast" /ApEinfo_fwdcolor="#ff51d8" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2630..2659 /label=GGSGSGGSGSGSGGS linker /ApEinfo_fwdcolor="#fff679" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2576..2602 /label=HA tag /ApEinfo_fwdcolor="#3a23ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1681..1681 /label=T>C silent H /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2029..2029 /label=G>A silent K /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtTGTACA tagtaatcaa ttacggggtc attagttcat 181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 301 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta 361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact 721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa 781 gcttgccacc atgGTCTTCA CACTCGAAGA TTTCGTTGGG GACTGGCGAC AGACAGCCGG 841 CTACAACCTG GACCAAGTCC TTGAACAGGG AGGTGTGTCC AGTTTGTTTC AGAATCTCGG 901 GGTGTCCGTA ACTCCGATCC AAAGGATTGT CCTGAGCGGT GAAAATGGGC TGAAGATCGA 961 CATCCATGTC ATCATCCCGT ATGAAGGTCT GAGCGGCGAC CAAATGGGCC AGATCGAAAA 1021 AATTTTTAAG GTGGTGTACC CTGTGGATGA TCATCACTTT AAGGTGATCC TGCACTATGG 1081 CACACTGGTA ATCGACGGGG TTACGCCGAA CATGATCGAC TATTTCGGAC GGCCGTATGA 1141 AGGCATCGCC GTGTTCGACG GCAAAAAGAT CACTGTAACA GGGACCCTGT GGAACGGCAA 1201 CAAAATTATC GACGAGCGCC TGATCAACCC CGACGGCTCC CTGCTGTTCC GAGTAACCAT 1261 CAACGGAGTG ACCGGCTGGC GGCTGTGCGA ACGCATTCTG GCGggcgacc cactagtcca 1321 gtgtggtgga attgccggat ccgccaccGG TGAAAAACCC GGGACCAGGG TCTTCAAGAA 1381 GTCGAGCCCT AACTGCAAGC TCACCGTGTA CTTGGGCAAG CGTGACTTTG TGGATCACTT 1441 GGACAAAGTG GATCCTGTCG ATGGTGTGGT GCTTGTGGAT CCTGACTACT TGAAGGACCG 1501 GAAAGTGTTT GTGACCCTCA CCTGTGCCTT CCGCTATGGC CGAGAAGACC TGGATGTACT 1561 GGGCCTGTCT TTCCGCAAAG ATCTGTTCAT CGCCACCTAC CAGGCCTTCC CCCCCATGCC 1621 CAACCCACCT CGGCCCCCCA CCCGCCTACA GGACCGACTG CTGAAGAAGT TGGGCCAGCA 1681 CGCCCACCCC TTTTTTTTCA CAATACCCCA GAATTTGCCT TGCTCCGTCA CACTGCAGCC 1741 AGGACCGGAG GACACAGGGA AGGCCTGTGG AGTAGACTTT GAGATTCGAG CCTTCTGTGC 1801 CAAATCTATA GAAGAAAAAA GCCACAAAAG GAACTCCGTG CGGCTTATCA TCAGAAAGGT 1861 ACAGTTTGCT CCTGAGACAC CCGGCCCCCA GCCATCAGCT GAAACCACAC GCCACTTCCT 1921 CATGTCTGAC CGGAGGTCCC TGCACCTAGA GGCTTCCCTG GACAAAGAGC TGTACTACCA 1981 TGGGGAACCC CTCAATGTCA ACGTCCACGT CACCAACAAT TCTGCCAAAA CCGTCAAGAA 2041 GATCAGAGTG TCTGTGAGAC AGTATGCCGA CATTTGCCTC TTCAGCACCG CGCAGTACAA 2101 GTGTCCTGTG GCTCAGCTTG AACAAGATGA CCAGGTGTCT CCCAGTTCCA CATTCTGCAA 2161 GGTGTACACC ATAACCCCGC TGCTCAGTGA CAACCGAGAG AAGCGTGGCC TTGCCCTTGA 2221 TGGGCAACTC AAGCACGAAG ACACCAACCT GGCTTCCAGC ACCATTGTGA AGGAGGGAGC 2281 CAACAAGGAG GTGCTGGGAA TCCTAGTATC CTACAGGGTC AAGGTGAAGC TGGTGGTGTC 2341 TCGAGGCGGG GATGTCTCCG TGGAGCTACC TTTCGTCCTA ATGCACCCCA AGCCCCACGA 2401 CCACATCACC CTTCCCCGAC CCCAGTCAGC CCCCCGGGAA ATAGACATCC CTGTGGATAC 2461 CAACCTCATT GAATTCGATA CCAACTATGC CACAGACGAC GACATCGTGT TTGAGGACTT 2521 TGCGAGGCTT CGGCTGAAGG GGATGAAGGA TGACGACTGT GATGACCAGT TCTGCTACCC 2581 ATACGATGTG CCAGATTACG CCtatcccta tgacgtccct gattacgctG GTGGAAGTGG 2641 ATCAGGCAGC GGTGGATCTG GAGAAAGCtt gtttaaggga ccacgtgatt acaacccgat 2701 atcgagcacc atttgtcatt tgacgaatga atctgatggg cacacaacat cgttgtatgg 2761 tattggattt ggtcccttca tcattacaaa caagcacttg tttAGAAGAa ataatggaac 2821 actgttggtc caatcactac atggtgtatt caaggtcaag aacaccacga ctttgcaaca 2881 acacctcatt gatgggaggg acatgataat tattcgcatg cctaaggatt tcccaccatt 2941 tcctcaaaag ctgaaattta gagagccaca aagggaagag cgcatatgtc ttgtgacaac 3001 caacttccaa actaagagca tgtctagcat ggtgtcagac actagttgca cattcccttc 3061 atctgatggc atattctgga agcattggat tcaaaccaag gatgggcagt gtggcagtcc 3121 attagtatca actagagatg ggttcattgt tggtatacac tcagcatcga atttcaccaa 3181 cacaaacaat tatttcacaa gcgtgccgaa aaacttcatg gaattgttga caaatcagga 3241 ggcgcagcag tgggttagtg gttggcgatt aaatgctgac tcagtattgt ggggtggcca 3301 taaagttttc atggtgTAAG AATTCctcga gCTAGAGCTC GCTGATCAGC CTCGACTGTG 3361 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA 3421 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT 3481 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA 3541 GACAATAGCA GGCATGCTGG GGAggtaacc acgtgcggac cgagcggccg caggaacccc 3601 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac 3661 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca 3721 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtattcaca 3781 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt 3841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 3901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 3961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 4021 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 4081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 4141 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 4201 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 4261 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 4321 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 4381 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 4441 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 4501 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 4561 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 4621 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 4681 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 4741 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 4801 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 4861 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 4921 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 4981 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 5041 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 5101 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 5161 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 5221 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 5281 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 5341 aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt 5401 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 5461 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 5521 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 5581 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 5641 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 5701 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 5761 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 5821 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 5881 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 5941 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 6001 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 6061 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 6121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 6181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 6241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 6301 gccttttgct ggccttttgc tcacatgt //