LOCUS       Exported               10000 bp ds-DNA     circular SYN 30-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 28, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
COMMENT     pLX208-CaM-2xHA-TEV219 from 1 to 9415
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..413
                     /label=5'LTR
                     /note="/vntifkey=19"
     misc_feature    464..601
                     /label=psi
                     /note="/vntifkey=21"
     misc_feature    568..932
                     /label=gag
                     /note="/vntifkey=21"
     misc_feature    1078..1319
                     /label=RRE
                     /note="/vntifkey=21"
     CDS             1707..1802
                     /label=Hygro
                     /note="/vntifkey=4"
     misc_feature    1847..1964
                     /label=cPPT
                     /note="/vntifkey=21"
     promoter        2023..2604
                     /label=CMV
                     /note="/vntifkey=29"
     misc_feature    2605..2610
                     /label=BamHI
                     /label=BamHI(1)
     misc_feature    2617..2619
                     /label=START
     misc_feature    2620..2667
                     /label=gLuc signal peptide
     misc_feature    2668..3177
                     /label=Nanoluc
     misc_feature    3178..3204
                     /label=HA tag
     exon            3205..4500
                     /label=opto-b2AR
     misc_feature    4501..4560
                     /label=TS
     misc_feature    4567..4572
                     /label=NheI
     misc_feature    4573..4578
                     /label=asLOV2
                     /label=asLOV2(1)
     misc_feature    4579..4581
                     /label=L2R
     misc_feature    join(4582..4608,4612..4656,4660..4923,4927..4932)
                     /label=asLOV2
     misc_feature    4609..4611
                     /label=N12S
     misc_feature    4657..4659
                     /label=A28V
     misc_feature    4924..4926
                     /label=H117R
     misc_feature    join(4933..4950,4954..4962,4966..4980)
                     /label=asLOV2
                     /label=asLOV2(2)
     misc_feature    join(4933..4950,4954..4962,4966..4980)
                     /label=asLOV2 Ja helix
     misc_feature    4951..4953
                     /label=G528A
     misc_feature    4963..4965
                     /label=I130V
     misc_feature    4981..4983
                     /label=N538E
     misc_feature    4984..5001
                     /label=asLOV2
                     /label=asLOV2(3)
     misc_feature    4984..5001
                     /label=asLOV2 Ja helix
                     /label=asLOV2 Ja helix(1)
     misc_feature    5002..5022
                     /label=TEVcs ENLYFQL
     misc_feature    5029..5034
                     /label=BamHI
     misc_feature    5035..5058
                     /label=Flag
     misc_feature    5059..5838
                     /label=GAL4
     misc_feature    5839..5841
                     /label=STOP
     misc_feature    5842..5847
                     /label=MluI
     misc_feature    5857..6445
                     /label=WPRE
                     /note="/vntifkey=21"
     LTR             6516..6751
                     /label=3' LTR SIN
                     /label=3'\LTR\SIN
                     /note="/vntifkey=19"
     rep_origin      7305..7735
                     /label=F1 ORI
                     /label=F1\ORI
                     /note="/vntifkey=33"
     misc_feature    8106..8912
                     /label=AMP r
                     /label=AMP\r
                     /note="/vntifkey=21"
     rep_origin      complement(9038..9653)
                     /direction=LEFT
                     /label=pUC Origin
                     /label=pUC\Origin
                     /note="/vntifkey=33"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagagcg ctgccgtctg gaccgatggc tgtgtggaag
     1741 tgctcgccga tagtggaaac agacgcccca gcactcgtcc tagggcaaag gatctgcagt
     1801 aatgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1861 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1921 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1981 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2041 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2161 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2221 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2281 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2341 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2401 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2461 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2521 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2581 gcagagctct ctggctaact gtcgggatcc gccaccatgg gagtcaaagt tctgtttgcc
     2641 ctgatctgca tcgctgtggc cgaggccgtc ttcacactcg aagatttcgt tggggactgg
     2701 cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg
     2761 tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat
     2821 gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg
     2881 ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg
     2941 atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc
     3001 ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc
     3061 ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg
     3121 ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtat
     3181 ccgtacgacg taccagacta cgcaaacgga acagagggcc caaactttta cgttcccttc
     3241 tccaataaga ctggggtcgt gagaagccca tttgaggcgc ctcaatacta ccttgctgag
     3301 ccgtggcagt tttctatgct cgctgcttac atgttcttgc tgatcatgct ggggttccct
     3361 atcaatttcc tgacgctgta cgttatagca aagttcgaac gcctccaaac cgtgttgaac
     3421 tacatactcc ttaacctcgc ggttgccgac ctcttcatgg ttttcggggg tttcaccacc
     3481 accctctaca cctcccttca cggctacttc gtgttcggcc ctaccggatg caatctggaa
     3541 ggctttttcg caacgctggg gggggagatt gccctttgga gcctggtggt cttggccata
     3601 gagaggtacg tggtggtcac atccccattc aagtaccaga gtttgcttac aaagaacaag
     3661 gctatcatgg gggtcgcctt cacatgggtg atggcgctgg cttgcgctgc cccaccgctg
     3721 gtaggctggt cccggtatat tccggaggga atgcagtgca gttgtgggat cgactactac
     3781 accccacacg aagagactaa caacgagtct tttgtgattt atatgttcgt ggtccacttc
     3841 atcatccccc tgatagtgat ctttttctgt tacggcaggg tgttccaggt cgccaaaagg
     3901 cagctccaga agatcgacaa aagcgaaggc cgctttcaca gccccaatct tggacaggtt
     3961 gaacaggacg gcaggtcagg gcacgggctg cgacgcagtt ctaagttctg cctgaaggaa
     4021 cataaggcct tgagaatggt gatcatcatg gtaatcgcct tcctgatatg ctggcttcca
     4081 tacgctggcg tggcttttta tatattcacg caccaggggt cagattttgg gcctatcttt
     4141 atgaccatac ctgctttctt cgctaagacg agtgcggtgt ataacccagt gatatacatc
     4201 atgatgaaca aacaattcag aattgccttc caggaattgc tctgtctcag acgcagctct
     4261 tccaaagcgt acggaaatgg ctattcatct aacagcaacg gaaagactga ttatatgggc
     4321 gaagccagtg gctgccagct gggccaggaa aaagagagcg agcggctttg tgaagatccc
     4381 ccaggcactg agagcttcgt gaattgtcag ggaacagttc cgagtctctc tcttgattca
     4441 cagggacgca attgctctac caacgacagc cccctggaga cttcccaggt cgctccggcc
     4501 aagagcagga tcaccagcga gggcgagtac atccccctgg accagatcga catcaacgtg
     4561 ggtggagcta gcggcagccg ggcgaccacc ctggaacgta ttgaaaagag ctttgtgatt
     4621 accgatccgc gtctgccgga taacccgatt atttttgtga gcgatagctt tctgcagctg
     4681 accgaatata gccgtgaaga aattctgggc cgtaactgcc gttttctgca gggcccggaa
     4741 accgatcgtg cgaccgtgcg taaaattcgt gatgcgattg ataaccagac cgaagtgacc
     4801 gtgcagctga ttaactatac caaaagcggc aaaaaatttt ggaacctgtt tcatctgcag
     4861 ccgatgcgtg atcagaaagg cgatgtgcag tattttattg gcgtgcagct ggatggtacc
     4921 gaacgtgtgc gtgatgcggc ggaacgtgaa gctgtgatgc tggtcaagaa aaccgcggag
     4981 gaaatcgacg aagccgctaa ggaaaatctc tacttccaga tgggcggagg atccgactac
     5041 aaggatgacg acgataagaa gctactgtct tctatcgaac aagcatgcga tatttgccga
     5101 cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac
     5161 tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca
     5221 gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa
     5281 gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga
     5341 ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag
     5401 actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag
     5461 agtagtaaca aaggtcaaag acagttgact gtatcggcca attttaatca aagtgggaat
     5521 attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc gaacctcata
     5581 acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat
     5641 aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca
     5701 ctgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg
     5761 tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca
     5821 ccaaacccaa aaaaagagta gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6241 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6421 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     6481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6721 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     6781 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     6841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6961 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7021 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7081 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     7141 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7201 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7261 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7321 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7381 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7441 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7501 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7561 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7621 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7681 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7741 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     7801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7921 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7981 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8041 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8101 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8161 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8221 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8281 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8341 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8401 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8461 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8521 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8581 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8641 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8701 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8761 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9721 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9781 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9841 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9901 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9961 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
