LOCUS APP6_beta2_AR_NN 6910 bp ds-DNA circular 09-FEB-2019 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 1..130 /label=ITR /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 870..871 /label=Mutation /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3044..3049 /label=BamHI /ApEinfo_fwdcolor="#0000ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(2597..2623,2627..2671,2675..2938,2942..2947) /label=asLOV2 /ApEinfo_fwdcolor="#f746ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 753..769 /label=T7 promoter primer /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2066..2575 /label=Nanoluc /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2966..2968 /label=G528A /ApEinfo_fwdcolor="#ffe19f" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4174..4314 /label=ITR(1) /ApEinfo_label="ITR" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2978..2980 /label=I130V /ApEinfo_fwdcolor="#ffe950" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(3854..3861,3863..3867,3869..3873,3875..3894) /label=V5 tag /ApEinfo_fwdcolor="#ff80ff" /ApEinfo_revcolor="#ff80ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 785..786 /label=NotI /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="#ff8040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3914..4145 /label=PolyA /ApEinfo_fwdcolor="#ff2549" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2582..2587 /label=nhe1 /ApEinfo_fwdcolor="#ff00ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2588..2593 /label=asLOV2(1) /ApEinfo_label="asLOV2" /ApEinfo_fwdcolor="#f746ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 151..708 /label=CMV promoter /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2594..2596 /label=L2R /ApEinfo_fwdcolor="#ffe85a" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3050..3073 /label=Flag /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2030..2065 /label=NNES /ApEinfo_fwdcolor="#fffb76" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2996..2998 /label=N538E /ApEinfo_fwdcolor="#ff9e3f" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(2948..2965,2969..2977,2981..2995) /label=asLOV2(2) /ApEinfo_label="asLOV2" /ApEinfo_fwdcolor="#f746ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2999..3016 /label=asLOV2(3) /ApEinfo_label="asLOV2" /ApEinfo_fwdcolor="#f746ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3074..3853 /label=GAL4 /ApEinfo_fwdcolor="#f026ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2624..2626 /label=N12S /ApEinfo_fwdcolor="#ffd85f" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2672..2674 /label=A28V /ApEinfo_fwdcolor="#ffe04d" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3902..3907 /label=EcoRI /ApEinfo_fwdcolor="#46ff6e" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3017..3037 /label=TEVcs ENLYFQL /ApEinfo_fwdcolor="#76cbff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(3899..3901) /label=double stop /ApEinfo_fwdcolor="#800080" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(2948..2965,2969..2977,2981..2995) /label=asLOV2 Ja helix /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2939..2941 /label=H117R /ApEinfo_fwdcolor="#ffe369" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2582..2587 /label=NheI /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2999..3016 /label=asLOV2 Ja helix(1) /ApEinfo_label="asLOV2 Ja helix" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 791..2029 /label=beta2-AR /ApEinfo_fwdcolor="#ff9a56" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 836..838 /label=G>R /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 869..871 /label=E>Q /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtTGTACA tagtaatcaa ttacggggtc attagttcat 181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 301 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta 361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact 721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa 781 gcttgccacc atggggcaac ccgggaacgg cagcgccttc ttgctggcac ccaatAgaag 841 ccatgcgccg gaccacgacg tcacgcagCa Gagggacgag gtgtgggtgg tgggcatggg 901 catcgtcatg tctctcatcg tcctggccat cgtgtttggc aatgtgctgg tcatcacagc 961 cattgccaag ttcgagcgtc tgcagacggt caccaactac ttcatcactt cactggcctg 1021 tgctgatctg gtcatgggcc tggcagtggt gccctttggg gccgcccata ttcttatgaa 1081 aatgtggact tttggcaact tctggtgcga gttttggact tccattgatg tgctgtgcgt 1141 cacggccagc attgagaccc tgtgcgtgat cgcagtggat cgctactttg ccattacttc 1201 acctttcaag taccagagcc tgctgaccaa gaataaggcc cgggtgatca ttctgatggt 1261 gtggattgtg tcaggcctta cctccttctt gcccattcag atgcactggt accgggccac 1321 ccaccaggaa gccatcaact gctatgccaa tgagacctgc tgtgacttct tcacgaacca 1381 agcctatgcc attgcctctt ccatcgtgtc cttctacgtt cccctggtga tcatggtctt 1441 cgtctactcc agggtctttc aggaggccaa aaggcagctc cagaagattg acaaatctga 1501 gggccgcttc catgtccaga accttagcca ggtggagcag gatgggcgga cggggcatgg 1561 actccgcaga tcttccaagt tctgcttgaa ggagcacaaa gccctcaaga cgttaggcat 1621 catcatgggc actttcaccc tctgctggct gcccttcttc atcgttaaca ttgtgcatgt 1681 gatccaggat aacctcatcc gtaaggaagt ttacatcctc ctaaattgga taggctatgt 1741 caattctggt ttcaatcccc ttatctactg ccggagccca gatttcagga ttgccttcca 1801 ggagcttctg tgcctgcgca ggtcttcttt gaaggcctat gggaatggct actccagcaa 1861 cggcaacaca ggggagcaga gtggatatca cgtggaacag gagaaagaaa ataaactgct 1921 gtgtgaagac ctcccaggca cggaagactt tgtgggccat caaggtactg tgcctagcga 1981 taacattgat tcacaaggga ggaattgtag tacaaatgac tcactgctgG AGCTGGCCGA 2041 GAAGCTGGCC GGCCTGGACA TCAACGTCTT CACACTCGAA GATTTCGTTG GGGACTGGCG 2101 ACAGACAGCC GGCTACAACC TGGACCAAGT CCTTGAACAG GGAGGTGTGT CCAGTTTGTT 2161 TCAGAATCTC GGGGTGTCCG TAACTCCGAT CCAAAGGATT GTCCTGAGCG GTGAAAATGG 2221 GCTGAAGATC GACATCCATG TCATCATCCC GTATGAAGGT CTGAGCGGCG ACCAAATGGG 2281 CCAGATCGAA AAAATTTTTA AGGTGGTGTA CCCTGTGGAT GATCATCACT TTAAGGTGAT 2341 CCTGCACTAT GGCACACTGG TAATCGACGG GGTTACGCCG AACATGATCG ACTATTTCGG 2401 ACGGCCGTAT GAAGGCATCG CCGTGTTCGA CGGCAAAAAG ATCACTGTAA CAGGGACCCT 2461 GTGGAACGGC AACAAAATTA TCGACGAGCG CCTGATCAAC CCCGACGGCT CCCTGCTGTT 2521 CCGAGTAACC ATCAACGGAG TGACCGGCTG GCGGCTGTGC GAACGCATTC TGGCGGGTGG 2581 AgctagcGGC AGCcggGCGA CCACCCTGGA ACGTATTGAA AAGagcTTTG TGATTACCGA 2641 TCCGCGTCTG CCGGATAACC CGATTATTTT TgtgAGCGAT AGCTTTCTGC AGCTGACCGA 2701 ATATAGCCGT GAAGAAATTC TGGGCCGTAA CTGCCGTTTT CTGCAGGGCC CGGAAACCGA 2761 TCGTGCGACC GTGCGTAAAA TTCGTGATGC GATTGATAAC CAGACCGAAG TGACCGTGCA 2821 GCTGATTAAC TATACCAAAA GCGGCAAAAA ATTTTGGAAC CTGTTTCATC TGCAGCCGAT 2881 GCGTGATCAG AAAGGCGATG TGCAGTATTT TATTGGCGTG CAGCTGGATG GTACCGAAcg 2941 tGTGCGTGAT GCGGCGGAAC GTGAAGCTGT GATGCTGgtc AAGAAAACCG CGgagGAAat 3001 cgacgaagcc gctaagGAAA ATCTCTACTT CCAGATGGGC GGAggatccG ACTACAAGGA 3061 TGACGACGAT AAGaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa 3121 aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga 3181 gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt 3241 ggaatcaagg ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct 3301 tgacatgatt ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt 3361 tgtacaagat aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga 3421 tatgcctcta acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag 3481 taacaaaggt caaagacagt tgactgtatc ggccaatttt aatcaaagtg ggaatattgc 3541 tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc tcataacaac 3601 tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc atgataactt 3661 catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa aaccactgtc 3721 acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag ggatgtttaa 3781 taccactaca atggatgatg tatataacta tctattcgat gatgaagata ccccaccaaa 3841 cccaaaaaaa gagGGCAAGC CGATCCCTAA CCCtCTGCTG GGCCTGGACA GCACTtagTA 3901 AGAATTCctc gagCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA 3961 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC 4021 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG 4081 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT 4141 GGGGAggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca 4201 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc 4261 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc 4321 tgatgcggta ttttctcctt acgcatctgt gcggtattca caccgcatac gtcaaagcaa 4381 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 4441 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 4501 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 4561 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt 4621 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 4681 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt 4741 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 4801 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt 4861 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac 4921 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 4981 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc 5041 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 5101 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 5161 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 5221 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 5281 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 5341 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 5401 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 5461 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 5521 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 5581 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 5641 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 5701 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 5761 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 5821 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 5881 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 5941 cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 6001 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 6061 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 6121 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 6181 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 6241 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 6301 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 6361 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 6421 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 6481 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 6541 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 6601 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 6661 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 6721 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 6781 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 6841 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 6901 gctcacatgt //