LOCUS       Exported                9935 bp ds-DNA     circular SYN 03-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Apr 3, 2019 from SnapGene 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1681..1914
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2417..2534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2548..2570
                     /label=EF-1-alpha_NheIKpnI.fwd
     promoter        2590..3752
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     2738..2755
                     /label=pWPTFor
     primer_bind     2738..2755
                     /label=pWPTFor
     intron          2821..3743
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3623..3641
                     /label=CS_EF1_F
     primer_bind     3623..3641
                     /label=CSII_EF1_F
     primer_bind     3771..3796
                     /label=mCherry_EcoRI.fwd
     CDS             complement(3772..4602)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="NLSRSGGSYLSLLFWIYLSLLFWIYLSLLFWISSRSESGLVQLVH
                     AAGGVAALGAFVLFHDGVVLVVGGDVQLDVDVVGAGQLHGLLGLVGGLDLSVVVAAVLQ
                     LQPLLDLALQGAVLGVHPLGGGLPAHGLLLHYGAVGGEVGAAQLHLVDELAVLQGGVLG
                     HGHHAAVLEVHHALPLEALGEGQLQVVGDVGGVLHVGLGAVHELRGQDVPGEGQGATLG
                     HLQLGGLGALVGAALALALDLELVAVHGALHVHLEAHELLDDGHVILLALAHHGI"
     primer_bind     3776..3796
                     /label=mCherry_NheI.fwd
     CDS             3778..4599
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKSGLRSRADPKKKRKVDPKKKRKVDPKKKRKVGSTGSR"
     gene            3781..4485
                     /gene="mCherry"
                     /product="This mCherry is mammalian optimized and should 
                     work in both mouse and human cells."
                     /label=mCherry
     misc_feature    3958..4011
                     /label=Q66M (mCherry)
     primer_bind     complement(4461..4485)
                     /label=mCherry_NotI.rev
     misc_feature    4510..4530
                     /label=SV40 NLS
     misc_feature    4516..4530
                     /label=NLS
     misc_feature    4534..4554
                     /label=SV40 NLS
     misc_feature    4558..4581
                     /label=SV40 NLS
     primer_bind     complement(4569..4607)
                     /label=mCherry-NLS_HpaI.rev
     misc_feature    4629..5217
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     primer_bind     complement(4682..4702)
                     /label=WPRE-R
     primer_bind     complement(4704..4721)
                     /label=pWPTRev
     primer_bind     complement(4704..4721)
                     /label=pWPTRev
     CDS             complement(5100..5111)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5736..5916
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5948..6172
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6218..6646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6660..6990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6841..6976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7038..7085
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             7104..7478
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7608..7729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7778..7794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7802..7818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7826..7856)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7871..7892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8180..8768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8939..9799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9800..9904)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     1561 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1621 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1681 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1741 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1801 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1861 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1921 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1981 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2041 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2101 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2161 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2221 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2281 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2341 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctacaaatgg
     2401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2521 aaattcaaaa ttttcgggtt tattacaggg acagcagaaa ttcactttga attaattcaa
     2581 gcttcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2641 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2701 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2761 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2821 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     2881 ccttgaatta cttccacctg gctccagtac gtgattcttg atcccgagct ggagccaggg
     2941 gcgggccttg cgctttagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     3001 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     3061 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     3121 gtcttgtaaa tgcgggccag gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     3181 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     3241 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     3301 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     3361 gagcggaaag atggccgctt cccggccctg ctccaggggg ctcaaaatgg aggacgcggc
     3421 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aggggccttt ccgtcctcag
     3481 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3541 ggagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3601 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3661 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3721 aaagtttttt tcttccattt caggtgtcgt gaacacgcta ccggtctcga gaattccatg
     3781 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     3841 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     3901 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     3961 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     4021 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     4081 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     4141 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     4201 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     4261 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     4321 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     4381 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     4441 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccgg actcagatct
     4501 cgagctgatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtagatcca
     4561 aaaaagaaga gaaaggtagg atccaccgga tctagataag ttaacatcga gggatcaagc
     4621 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4681 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4741 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4801 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4861 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4921 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4981 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5041 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5101 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5161 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5221 gataccgtcg agacctggaa aaacatggag caatcacaag tagcaacaca gcagctacca
     5281 atgctgcttg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     5341 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5401 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     5461 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     5521 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     5581 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     5641 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     5701 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     5761 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5821 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5881 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     5941 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6001 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6061 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6121 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6181 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6241 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6301 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6361 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6421 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6481 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6541 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
     6601 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6661 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
     6721 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6781 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6841 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6901 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6961 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7021 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     7081 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     7141 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     7201 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     7261 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     7321 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     7381 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     7441 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     7501 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7561 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7621 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7681 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7741 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7801 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7861 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7921 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7981 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8341 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     8401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9901 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
