LOCUS pHR158_pCMV_K133 8712 bp DNA circular UNA 02-MAR-2018 DEFINITION . ACCESSION urn.local...603-8q87g7q VERSION urn.local...603-8q87g7q KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 132..335 /note="Geneious type: Primer" /standard_name="CMV-promoter(Cepko)" misc_feature 219..243 /note="Geneious type: Primer" /standard_name="HRSeq_17" primer_bind 219..243 /standard_name="HRSeq17" primer_bind 286..306 /standard_name="QB0011" promoter 287..356 /standard_name="CMV promoter" primer_bind 293..314 /standard_name="QB0767" primer_bind 332..356 /standard_name="QB0042" misc_feature 336 /note="Geneious type: mismatch" /standard_name="G/C" primer_bind 377..396 /standard_name="QB0105" CDS 409..>411 /standard_name="Cas9" misc_feature 413..5718 /note="Geneious type: Editing History Replacement" /standard_name="ATAAAAAGTATTCTATTGGTTTAGACATCGGCACTAATTCCG TTGGATGGGCTGTCATAACCGATGAATACAAAGTACCTTCAAAGAAATTTAAGGTGTT GGGGAACACAGACCGTCATTCGATTAAAAAGAATCTTATCGGTGCCCTCCTATTCGAT AGTGGCGAAACGGCAGAGGCGACTCGCCTGAAACGAACCGCTCGGAGAAGGTATACAC GTCGCAAGAACCGAATATGTTACTTACAAGAAATTTTTAGCAATGAGATGGCCAAAGT TGACGATTCTTTCTTTCACCGTTTGGAAGAGTCCTTCCTTGTCGAAGAGGACAAGAAA CATGAACGGCACCCCATCTTTGGAAACATAGTAGATGAGGTGGCATATCATGAAAAGT ACCCAACGATTTATCACCTCAGAAAAAAGCTAGTTGACTCAACTGATAAAGCGGACCT GAGGTTAATCTACTTGGCTCTTGCCCATATGATAAAGTTCCGTGGGCACTTTCTCATT GAGGGTGATCTAAATCCGGACAACTCGGATGTCGACAAACTGTTCATCCAGTTAGTAC AAACCTATAATCAGTTGTTTGAAGAGAACCCTATAAATGCAAGTGGCGTGGATGCGAA GGCTATTCTTAGCGCCCGCCTCTCTAAATCCCGACGGCTAGAAAACCTGATCGCACAA TTACCCGGAGAGAAGAAAAATGGGTTGTTCGGTAACCTTATAGCGCTCTCACTAGGCC TGACACCAAATTTTAAGTCGAACTTCGACTTAGCTGAAGATGCCAAATTGCAGCTTAG TAAGGACACGTACGATGACGATCTCGACAATCTACTGGCACAAATTGGAGATCAGTAT GCGGACTTATTTTTGGCTGCCAAAAACCTTAGCGATGCAATCCTCCTATCTGACATAC TGAGAGTTAATACTGAGATTACCAAGGCGCCGTTATCCGCTTCAATGATCAAAAGGTA CGATGAACATCACCAAGACTTGACACTTCTCAAGGCCCTAGTCCGTCAGCAACTGCCT GAGAAATATAAGGAAATATTCTTTGATCAGTCGAAAAACGGGTACGCAGGTTATATTG ACGGCGGAGCGAGTCAAGAGGAATTCTACAAGTTTATCAAACCCATATTAGAGAAGAT GGATGGGACGGAAGAGTTGCTTGTAAAACTCAATCGCGAAGATCTACTGCGAAAGCAG CGGACTTTCGACAACGGTAGCATTCCACATCAAATCCACTTAGGCGAATTGCATGCTA TACTTAGAAGGCAGGAGGATTTTTATCCGTTCCTCAAAGACAATCGTGAAAAGATTGA GAAAATCCTAACCTTTCGCATACCTTACTATGTGGGACCCCTGGCCCGAGGGAACTCT CGGTTCGCATGGATGACAAGAAAGTCCGAAGAAACGATTACTCCATGGAATTTTGAGG AAGTTGTCGATAAAGGTGCGTCAGCTCAATCGTTCATCGAGAGGATGACCAACTTTGA CAAGAATTTACCGAACGAAAAAGTATTGCCTAAGCACAGTTTACTTTACGAGTATTTC ACAGTGTACAATGAACTCACGAAAGTTAAGTATGTCACTGAGGGCATGCGTAAACCCG CCTTTCTAAGCGGAGAACAGAAGAAAGCAATAGTAGATCTGTTATTCAAGACCAACCG CAAAGTGACAGTTAAGCAATTGAAAGAGGACTACTTTAAGAAAATTGAATGCTTCGAT TCTGTCGAGATCTCCGGGGTAGAAGATCGATTTAATGCGTCACTTGGTACGTATCATG ACCTCCTAAAGATAATTAAAGATAAGGACTTCCTGGATAACGAAGAGAATGAAGATAT CTTAGAAGATATAGTGTTGACTCTTACCCTCTTTGAAGATCGGGAAATGATTGAGGAA AGACTAAAAACATACGCTCACCTGTTCGACGATAAGGTTATGAAACAGTTAAAGAGGC GTCGCTATACGGGCTGGGGACGATTGTCGCGGAAACTTATCAACGGGATAAGAGACAA GCAAAGTGGTAAAACTATTCTCGATTTTCTAAAGAGCGACGGCTTCGCCAATAGGAAC TTTATGCAGCTGATCCATGATGACTCTTTAACCTTCAAAGAGGATATACAAAAGGCAC AGGTTTCCGGACAAGGGGACTCATTGCACGAACATATTGCGAATCTTGCTGGTTCGCC AGCCATCAAAAAGGGCATACTCCAGACAGTCAAAGTAGTGGATGAGCTAGTTAAGGTC ATGGGACGTCACAAACCGGAAAACATTGTAATCGAGATGGCACGCGAAAATCAAACGA CTCAGAAGGGGCAAAAAAACAGTCGAGAGCGGATGAAGAGAATAGAAGAGGGTATTAA AGAACTGGGCAGCCAGATCTTAAAGGAGCATCCTGTGGAAAATACCCAATTGCAGAAC GAGAAACTTTACCTCTATTACCTACAAAATGGAAGGGACATGTATGTTGATCAGGAAC TGGACATAAACCGTTTATCTGATTACGACGTCGATCACATTGTACCCCAATCCTTTTT GAAGGACGATTCAATCGACAATAAAGTGCTTACACGCTCGGATAAGAACCGAGGGAAA AGTGACAATGTTCCAAGCGAGGAAGTCGTAAAGAAAATGAAGAACTATTGGCGGCAGC TCCTAAATGCGAAACTGATAACGCAAAGAAAGTTCGATAACTTAACTAAAGCTGAGAG GGGTGGCTTGTCTGAACTTGACAAGGCCGGATTTATTAAACGTCAGCTCGTGGAAACC CGCCAAATCACAAAGCATGTTGCACAGATACTAGATTCCCGAATGAATACGAAATACG ACGAGAACGATAAGCTGATTCGGGAAGTCAAAGTAATCACTTTAAAGTCAAAATTGGT GTCGGACTTCAGAAAGGATTTTCAATTCTATAAAGTTAGGGAGATAAATAACTACCAC CATGCGCACGACGCTTATCTTAATGCCGTCGTAGGGACCGCACTCATTAAGAAATACC CGAAGCTAGAAAGTGAGTTTGTGTATGGTGATTACAAAGTTTATGACGTCCGTAAGAT GATCGCGAAAAGCGAACAGGAGATAGGCAAGGCTACAGCCAAATACTTCTTTTATTCT AACATTATGAATTTCTTTAAGACGGAAATCACTCTGGCAAACGGAGAGATACGCAAAC GACCTTTAATTGAAACCAATGGGGAGACAGGTGAAATCGTATGGGATAAGGGCCGGGA CTTCGCGACGGTGAGAAAAGTTTTGTCCATGCCCCAAGTCAACATAGTAAAGAAAACT GAGGTGCAGACCGGAGGGTTTTCAAAGGAATCGATTCTTCCAAAAAGGAATAGTGATA AGCTCATCGCTCGTAAAAAGGACTGGGACCCGAAAAAGTACGGTGGCTTCGATAGCCC TACAGTTGCCTATTCTGTCCTAGTAGTGGCAAAAGTTGAGAAGGGAAAATCCAAGAAA CTGAAGTCAGTCAAAGAATTATTGGGGATAACGATTATGGAGCGCTCGTCTTTTGAAA AGAACCCCATCGACTTCCTTGAGGCGAAAGGTTACAAGGAAGTAAAAAAGGATCTCAT AATTAAACTACCAAAGTATAGTCTGTTTGAGTTAGAAAATGGCCGAAAACGGATGTTG GCTAGCGCCGGAGAGCTTCAAAAGGGGAACGAACTCGCACTACCGTCTAAATACGTGA ATTTCCTGTATTTAGCGTCCCATTACGAGAAGTTGAAAGGTTCACCTGAAGATAACGA ACAGAAGCAACTTTTTGTTGAGCAGCACAAACATTATCTCGACGAAATCATAGAGCAA ATTTCGGAATTCAGTAAGAGAGTCATCCTAGCTGATGCCAATCTGGACAAAGTATTAA GCGCATACAACAAGCACAGGGATAAACCCATACGTGAGCAGGCGGAAAATATTATCCA TTTGTTTACTCTTACCAACCTCGGCGCTCCAGCCGCATTCAAGTATTTTGACACAACG ATAGATCGCAAACGATACACTTCTACCAAGGAGGTGCTAGACGCGACACTGATTCACC AATCCATCACGGGATTATATGAAACTCGGATAGATTTGTCACAGCTTGGGGGTGACGG ATCCCCCAAGAAGAAGAGGAAAGTCTCGAGCGACTACAAAGACCATGACGGTGATTAT AAAGATCATGACATCGATTACAAGGATGACGATGACAAGGCTGCAGGA" CDS 415..435 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /translation="PKKKRKV" /standard_name="SV40 NLS" misc_feature complement(429..449) /note="Geneious type: Primer" /standard_name="HR1074" misc_feature 431..449 /note="Geneious type: USER" /standard_name="USER" misc_feature 431..478 /note="Geneious type: Primer" /standard_name="HR1075" misc_feature 431..>459 /note="Geneious type: Primer" /standard_name="HR1073" misc_feature 436..459 /note="Geneious type: Linker" /standard_name="Linker from Fz Cas9" misc_feature <460..>1473 /note="Geneious type: Primer" /standard_name="gHR_08" misc_feature <460..1473 /note="Geneious type: Primer" /standard_name="hRad51 from pHR091" misc_feature <853..855 /note="Geneious type: Mutation" /standard_name="K133R" misc_feature complement(1454..1522) /note="Geneious type: Primer" /standard_name="HR1076" source <1474..>1569 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1474..1569 /note="Geneious type: Linker" /standard_name="BE4_linker" misc_feature 1508..1522 /note="Geneious type: USER" /standard_name="USER" misc_feature 1508..1600 /note="Geneious type: Primer" /standard_name="HR1070 (HR1071 for WT Cas9)" CDS 1570..5670 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL FEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTV KVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEH PVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVL TRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM PQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD" /standard_name=" FzCas9 NLS" misc_feature complement(1583..1598) /note="Geneious type: Primer" /standard_name="HR1063" misc_feature 1585..1598 /note="Geneious type: USER" /standard_name="USER" misc_feature 1585..1600 /note="Geneious type: Primer" /standard_name="HR1062" misc_feature 1594..>1596 /note="Geneious type: Mutation" /standard_name="D10A" misc_feature complement(1637..1657) /note="Geneious type: primer_bind_reverse" /standard_name="HRSeq75" misc_feature complement(1971..1992) /note="Geneious type: primer_bind_reverse" /standard_name="HRSeq06" primer_bind 1971..1992 /standard_name="HRSeq05" misc_feature complement(2816..2836) /note="Geneious type: primer_bind_reverse" /standard_name="HRSeq08" primer_bind 2816..2836 /standard_name="HRSeq07" primer_bind 3550..3567 /standard_name="HRSeq72" misc_feature complement(3732..3751) /note="Geneious type: primer_bind_reverse" /standard_name="HRSeq10" primer_bind 3732..3751 /standard_name="HRSeq09" misc_feature 4084..4086 /note="Geneious type: Mutation" /standard_name="H840" primer_bind 4531..4548 /standard_name="HRSeq73" misc_feature complement(4779..4797) /note="Geneious type: primer_bind_reverse" /standard_name="HRSeq12" primer_bind 4779..4797 /standard_name="HRSeq11" primer_bind complement(5173..5193) /note="EGFP, reverse primer" /standard_name="EGFP-N" primer_bind 5504..5524 /standard_name="HRSeq74" CDS 5671..5718 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /translation="KRPAATKKAGQAKKKK" /standard_name="nucleoplasmin NLS" misc_feature complement(5698..5719) /note="Geneious type: Primer" /standard_name="HR897" misc_feature complement(5767..5784) /note="Geneious type: primer_bind_reverse" /standard_name="QB0007" misc_feature complement(6029..6045) /note="Geneious type: primer_bind_reverse" /standard_name="QB0069" misc_feature 6044 /note="Geneious type: mismatch" /standard_name="A/C" misc_feature complement(6068..6084) /note="Geneious type: primer_bind_reverse" /standard_name="QB0047" misc_feature complement(6081..6103) /note="Geneious type: primer_bind_reverse" /standard_name="QB0049" primer_bind 6693..6711 /standard_name="QB0410" misc_feature complement(6818..6837) /note="Geneious type: primer_bind_reverse" /standard_name="QB0766" primer_bind 7635..7654 /standard_name="HRSeq71" primer_bind 7852..7871 /standard_name="QB0004" misc_feature complement(8273..8292) /note="Geneious type: primer_bind_reverse" /standard_name="QB0091" misc_feature complement(8340..8359) /note="Geneious type: Primer" /standard_name="HR1069" misc_feature 8344..8359 /note="Geneious type: USER" /standard_name="USER" misc_feature 8344..8364 /note="Geneious type: Primer" /standard_name="HR1068" misc_feature complement(8530..8549) /note="Geneious type: primer_bind_reverse" /standard_name="QB1109" enhancer 8540..131 /note="Geneious type: Enhancer" /standard_name="CMV-E" misc_feature complement(8544..8564) /note="Geneious type: primer_bind_reverse" /standard_name="HRSeq02" primer_bind 8544..8564 /standard_name="HRSeq01" misc_feature 8669 /note="Geneious type: mismatch" /standard_name="G" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggccccaaag 421 aagaagcgga aggtcggtat ccacggagtc ccagcagccg cgatgcagat gcagttggaa 481 gcgaatgcag atactagtgt cgaggaagag tcatttggcc cgcaacccat ctcgcgttta 541 gagcaatgtg gcatcaatgc aaacgatgtg aaaaaattag aggaagctgg attccacacg 601 gtcgaagcgg tcgcatacgc accgaaaaaa gagctgatca acatcaaagg catcagcgag 661 gcgaaagccg ataagattct tgcagaggcg gcgaaattag ttcccatggg atttacgacg 721 gcgactgagt tccatcaacg tcgttccgag atcattcaaa tcacgaccgg aagcaaggag 781 ttggataaac tgcttcaagg aggcatcgaa actggctcga tcactgaaat gtttggagag 841 tttcgtactg gccgaaccca gatctgccac acattggccg tcacttgcca gcttccgatc 901 gatcgtggtg ggggcgaggg gaaggccatg tacattgata ctgaggggac gtttcgtccc 961 gaacgccttc ttgcggtggc agagcgttac ggcttgtcag ggtctgatgt tcttgacaat 1021 gttgcctatg ctcgcgcatt taataccgac caccagacgc agcttctgta ccaagcaagc 1081 gcgatgatgg tagagtctcg ttacgcttta ttgattgttg actcagcaac agcgttgtac 1141 cgcacagatt attctgggcg cggcgaattg tcggcacgcc aaatgcattt agcccgcttt 1201 ttgcgtatgt tgctgcgcct tgcagacgaa tttggggtgg ctgtggtgat caccaaccaa 1261 gttgtagcac aggtggacgg tgccgctatg tttgccgctg atccgaaaaa acccatcgga 1321 gggaatatta tcgcacatgc atcgacaact cgcttatatc tgcgtaaggg ccgtggagaa 1381 acacgcatct gcaaaatcta tgactcccca tgcctgccgg aggcggaagc catgttcgca 1441 attaacgcag atggcgtagg ggacgccaaa gattctggtg gttcttctgg tggttctagc 1501 ggcagcgaga ctcccgggac ctcagagtcc gccacacccg aaagttctgg tggttcttct 1561 ggtggttctg acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg 1621 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc 1681 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca 1741 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg 1801 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc 1861 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc 1921 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg 1981 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg 2041 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac 2101 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa 2161 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag 2221 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc 2281 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg 2341 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg 2401 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac 2461 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc 2521 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc 2581 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc 2641 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc 2701 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg 2761 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag 2821 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa 2881 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga 2941 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc 3001 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc 3061 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc 3121 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc 3181 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa 3241 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg 3301 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg 3361 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat 3421 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc 3481 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc 3541 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca 3601 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac 3661 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat 3721 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg 3781 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac 3841 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc 3901 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa 3961 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat 4021 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg 4081 gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc 4141 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag 4201 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac 4261 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag 4321 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg 4381 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg 4441 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc 4501 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc 4561 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg 4621 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc 4681 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg 4741 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg 4801 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc 4861 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag 4921 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc 4981 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag 5041 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc 5101 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg 5161 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc 5221 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg 5281 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt 5341 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag 5401 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg 5461 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg 5521 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc 5581 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca 5641 cggatcgacc tgtctcagct gggaggcgac aaaaggccgg cggccacgaa aaaggccggc 5701 caggcaaaaa agaaaaagtg accggtcatc atcaccatca ccattgagtt taaacccgct 5761 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 5821 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 5881 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 5941 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 6001 ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt 6061 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 6121 tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat 6181 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 6241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 6301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 6361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 6421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 6481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 6541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 6601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 6661 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 6721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 6781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 6841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 6901 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 6961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 7021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 7081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 7141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 7201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 7261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 7321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 7381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 7441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 7501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 7561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 7621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 7681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 7741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 7801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 7861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 7921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 7981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 8041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 8101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 8161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 8221 acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct 8281 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 8341 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 8401 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc 8461 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 8521 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 8581 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 8641 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 8701 atcaagtgta tc //