LOCUS       pHR124_pCMV_hRad        4422 bp    DNA     circular UNA 13-NOV-2017
DEFINITION  Clone 425-51.
ACCESSION   urn.local...1mg-8az0rha
VERSION     urn.local...1mg-8az0rha
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     219..243
                     /standard_name="HRSeq17"
     primer_bind     286..306
                     /standard_name="QB0011"
     promoter        287..356
                     /standard_name="CMV promoter"
     primer_bind     293..314
                     /standard_name="QB0767"
     primer_bind     332..356
                     /standard_name="QB0042"
     misc_feature    336
                     /note="Geneious type: mismatch"
                     /standard_name="G/C"
     primer_bind     377..396
                     /standard_name="QB0105"
     misc_feature    complement(389..410)
                     /note="Geneious type: Primer"
                     /standard_name="HR881"
     misc_feature    399..410
                     /note="Geneious type: USER"
                     /standard_name="uHR_061"
     misc_feature    399..430
                     /note="Geneious type: Primer"
                     /standard_name="HR883"
     misc_feature    <412..>1425
                     /note="Geneious type: Primer"
                     /standard_name="gHR_08"
     misc_feature    <412..1425
                     /note="Geneious type: Primer"
                     /standard_name="hRad51 from pHR068"
     misc_feature    413..1426
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="ATAAAAAGTATTCTATTGGTTTAGACATCGGCACTAATTCCG
                     TTGGATGGGCTGTCATAACCGATGAATACAAAGTACCTTCAAAGAAATTTAAGGTGTT
                     GGGGAACACAGACCGTCATTCGATTAAAAAGAATCTTATCGGTGCCCTCCTATTCGAT
                     AGTGGCGAAACGGCAGAGGCGACTCGCCTGAAACGAACCGCTCGGAGAAGGTATACAC
                     GTCGCAAGAACCGAATATGTTACTTACAAGAAATTTTTAGCAATGAGATGGCCAAAGT
                     TGACGATTCTTTCTTTCACCGTTTGGAAGAGTCCTTCCTTGTCGAAGAGGACAAGAAA
                     CATGAACGGCACCCCATCTTTGGAAACATAGTAGATGAGGTGGCATATCATGAAAAGT
                     ACCCAACGATTTATCACCTCAGAAAAAAGCTAGTTGACTCAACTGATAAAGCGGACCT
                     GAGGTTAATCTACTTGGCTCTTGCCCATATGATAAAGTTCCGTGGGCACTTTCTCATT
                     GAGGGTGATCTAAATCCGGACAACTCGGATGTCGACAAACTGTTCATCCAGTTAGTAC
                     AAACCTATAATCAGTTGTTTGAAGAGAACCCTATAAATGCAAGTGGCGTGGATGCGAA
                     GGCTATTCTTAGCGCCCGCCTCTCTAAATCCCGACGGCTAGAAAACCTGATCGCACAA
                     TTACCCGGAGAGAAGAAAAATGGGTTGTTCGGTAACCTTATAGCGCTCTCACTAGGCC
                     TGACACCAAATTTTAAGTCGAACTTCGACTTAGCTGAAGATGCCAAATTGCAGCTTAG
                     TAAGGACACGTACGATGACGATCTCGACAATCTACTGGCACAAATTGGAGATCAGTAT
                     GCGGACTTATTTTTGGCTGCCAAAAACCTTAGCGATGCAATCCTCCTATCTGACATAC
                     TGAGAGTTAATACTGAGATTACCAAGGCGCCGTTATCCGCTTCAATGATCAAAAGGTA
                     CGATGAACATCACCAAGACTTGACACTTCTCAAGGCCCTAGTCCGTCAGCAACTGCCT
                     GAGAAATATAAGGAAATATTCTTTGATCAGTCGAAAAACGGGTACGCAGGTTATATTG
                     ACGGCGGAGCGAGTCAAGAGGAATTCTACAAGTTTATCAAACCCATATTAGAGAAGAT
                     GGATGGGACGGAAGAGTTGCTTGTAAAACTCAATCGCGAAGATCTACTGCGAAAGCAG
                     CGGACTTTCGACAACGGTAGCATTCCACATCAAATCCACTTAGGCGAATTGCATGCTA
                     TACTTAGAAGGCAGGAGGATTTTTATCCGTTCCTCAAAGACAATCGTGAAAAGATTGA
                     GAAAATCCTAACCTTTCGCATACCTTACTATGTGGGACCCCTGGCCCGAGGGAACTCT
                     CGGTTCGCATGGATGACAAGAAAGTCCGAAGAAACGATTACTCCATGGAATTTTGAGG
                     AAGTTGTCGATAAAGGTGCGTCAGCTCAATCGTTCATCGAGAGGATGACCAACTTTGA
                     CAAGAATTTACCGAACGAAAAAGTATTGCCTAAGCACAGTTTACTTTACGAGTATTTC
                     ACAGTGTACAATGAACTCACGAAAGTTAAGTATGTCACTGAGGGCATGCGTAAACCCG
                     CCTTTCTAAGCGGAGAACAGAAGAAAGCAATAGTAGATCTGTTATTCAAGACCAACCG
                     CAAAGTGACAGTTAAGCAATTGAAAGAGGACTACTTTAAGAAAATTGAATGCTTCGAT
                     TCTGTCGAGATCTCCGGGGTAGAAGATCGATTTAATGCGTCACTTGGTACGTATCATG
                     ACCTCCTAAAGATAATTAAAGATAAGGACTTCCTGGATAACGAAGAGAATGAAGATAT
                     CTTAGAAGATATAGTGTTGACTCTTACCCTCTTTGAAGATCGGGAAATGATTGAGGAA
                     AGACTAAAAACATACGCTCACCTGTTCGACGATAAGGTTATGAAACAGTTAAAGAGGC
                     GTCGCTATACGGGCTGGGGACGATTGTCGCGGAAACTTATCAACGGGATAAGAGACAA
                     GCAAAGTGGTAAAACTATTCTCGATTTTCTAAAGAGCGACGGCTTCGCCAATAGGAAC
                     TTTATGCAGCTGATCCATGATGACTCTTTAACCTTCAAAGAGGATATACAAAAGGCAC
                     AGGTTTCCGGACAAGGGGACTCATTGCACGAACATATTGCGAATCTTGCTGGTTCGCC
                     AGCCATCAAAAAGGGCATACTCCAGACAGTCAAAGTAGTGGATGAGCTAGTTAAGGTC
                     ATGGGACGTCACAAACCGGAAAACATTGTAATCGAGATGGCACGCGAAAATCAAACGA
                     CTCAGAAGGGGCAAAAAAACAGTCGAGAGCGGATGAAGAGAATAGAAGAGGGTATTAA
                     AGAACTGGGCAGCCAGATCTTAAAGGAGCATCCTGTGGAAAATACCCAATTGCAGAAC
                     GAGAAACTTTACCTCTATTACCTACAAAATGGAAGGGACATGTATGTTGATCAGGAAC
                     TGGACATAAACCGTTTATCTGATTACGACGTCGATCACATTGTACCCCAATCCTTTTT
                     GAAGGACGATTCAATCGACAATAAAGTGCTTACACGCTCGGATAAGAACCGAGGGAAA
                     AGTGACAATGTTCCAAGCGAGGAAGTCGTAAAGAAAATGAAGAACTATTGGCGGCAGC
                     TCCTAAATGCGAAACTGATAACGCAAAGAAAGTTCGATAACTTAACTAAAGCTGAGAG
                     GGGTGGCTTGTCTGAACTTGACAAGGCCGGATTTATTAAACGTCAGCTCGTGGAAACC
                     CGCCAAATCACAAAGCATGTTGCACAGATACTAGATTCCCGAATGAATACGAAATACG
                     ACGAGAACGATAAGCTGATTCGGGAAGTCAAAGTAATCACTTTAAAGTCAAAATTGGT
                     GTCGGACTTCAGAAAGGATTTTCAATTCTATAAAGTTAGGGAGATAAATAACTACCAC
                     CATGCGCACGACGCTTATCTTAATGCCGTCGTAGGGACCGCACTCATTAAGAAATACC
                     CGAAGCTAGAAAGTGAGTTTGTGTATGGTGATTACAAAGTTTATGACGTCCGTAAGAT
                     GATCGCGAAAAGCGAACAGGAGATAGGCAAGGCTACAGCCAAATACTTCTTTTATTCT
                     AACATTATGAATTTCTTTAAGACGGAAATCACTCTGGCAAACGGAGAGATACGCAAAC
                     GACCTTTAATTGAAACCAATGGGGAGACAGGTGAAATCGTATGGGATAAGGGCCGGGA
                     CTTCGCGACGGTGAGAAAAGTTTTGTCCATGCCCCAAGTCAACATAGTAAAGAAAACT
                     GAGGTGCAGACCGGAGGGTTTTCAAAGGAATCGATTCTTCCAAAAAGGAATAGTGATA
                     AGCTCATCGCTCGTAAAAAGGACTGGGACCCGAAAAAGTACGGTGGCTTCGATAGCCC
                     TACAGTTGCCTATTCTGTCCTAGTAGTGGCAAAAGTTGAGAAGGGAAAATCCAAGAAA
                     CTGAAGTCAGTCAAAGAATTATTGGGGATAACGATTATGGAGCGCTCGTCTTTTGAAA
                     AGAACCCCATCGACTTCCTTGAGGCGAAAGGTTACAAGGAAGTAAAAAAGGATCTCAT
                     AATTAAACTACCAAAGTATAGTCTGTTTGAGTTAGAAAATGGCCGAAAACGGATGTTG
                     GCTAGCGCCGGAGAGCTTCAAAAGGGGAACGAACTCGCACTACCGTCTAAATACGTGA
                     ATTTCCTGTATTTAGCGTCCCATTACGAGAAGTTGAAAGGTTCACCTGAAGATAACGA
                     ACAGAAGCAACTTTTTGTTGAGCAGCACAAACATTATCTCGACGAAATCATAGAGCAA
                     ATTTCGGAATTCAGTAAGAGAGTCATCCTAGCTGATGCCAATCTGGACAAAGTATTAA
                     GCGCATACAACAAGCACAGGGATAAACCCATACGTGAGCAGGCGGAAAATATTATCCA
                     TTTGTTTACTCTTACCAACCTCGGCGCTCCAGCCGCATTCAAGTATTTTGACACAACG
                     ATAGATCGCAAACGATACACTTCTACCAAGGAGGTGCTAGACGCGACACTGATTCACC
                     AATCCATCACGGGATTATATGAAACTCGGATAGATTTGTCACAGCTTGGGGGTGACGG
                     ATCCCCCAAGAAGAAGAGGAAAGTCTCGAGCGACTACAAAGACCATGACGGTGATTAT
                     AAAGATCATGACATCGATTACAAGGATGACGATGACAAGGCTGCAG"
     misc_feature    <805..807
                     /note="Geneious type: Mutation"
                     /standard_name="K133R"
     misc_feature    complement(1411..1442)
                     /note="Geneious type: Primer"
                     /standard_name="HR885"
     source          <1426..>1428
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1431..1442
                     /note="Geneious type: USER"
                     /standard_name="uHR_063"
     misc_feature    1431..1449
                     /note="Geneious type: Primer"
                     /standard_name="HR886"
     misc_feature    1433
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     misc_feature    1437
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    1439^1440
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="T"
     misc_feature    complement(1477..1494)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="QB0007"
     misc_feature    complement(1739..1755)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="QB0069"
     misc_feature    1754
                     /note="Geneious type: mismatch"
                     /standard_name="A/C"
     misc_feature    complement(1778..1794)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="QB0047"
     misc_feature    complement(1791..1813)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="QB0049"
     primer_bind     2403..2421
                     /standard_name="QB0410"
     misc_feature    complement(2528..2547)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="QB0766"
     primer_bind     3562..3581
                     /standard_name="QB0004"
     misc_feature    complement(3983..4002)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="QB0091"
     misc_feature    complement(4240..4259)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="QB1109"
     primer_bind     4254..4274
                     /standard_name="HRSeq01"
     misc_feature    complement(4254..4274)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="HRSeq02"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggcgatgcag
      421 atgcagttgg aagcgaatgc agatactagt gtcgaggaag agtcatttgg cccgcaaccc
      481 atctcgcgtt tagagcaatg tggcatcaat gcaaacgatg tgaaaaaatt agaggaagct
      541 ggattccaca cggtcgaagc ggtcgcatac gcaccgaaaa aagagctgat caacatcaaa
      601 ggcatcagcg aggcgaaagc cgataagatt cttgcagagg cggcgaaatt agttcccatg
      661 ggatttacga cggcgactga gttccatcaa cgtcgttccg agatcattca aatcacgacc
      721 ggaagcaagg agttggataa actgcttcaa ggaggcatcg aaactggctc gatcactgaa
      781 atgtttggag agtttcgtac tggccgaacc cagatctgcc acacattggc cgtcacttgc
      841 cagcttccga tcgatcgtgg tgggggcgag gggaaggcca tgtacattga tactgagggg
      901 acgtttcgtc ccgaacgcct tcttgcggtg gcagagcgtt acggcttgtc agggtctgat
      961 gttcttgaca atgttgccta tgctcgcgca tttaataccg accaccagac gcagcttctg
     1021 taccaagcaa gcgcgatgat ggtagagtct cgttacgctt tattgattgt tgactcagca
     1081 acagcgttgt accgcacaga ttattctggg cgcggcgaat tgtcggcacg ccaaatgcat
     1141 ttagcccgct ttttgcgtat gttgctgcgc cttgcagacg aatttggggt ggctgtggtg
     1201 atcaccaacc aagttgtagc acaggtggac ggtgccgcta tgtttgccgc tgatccgaaa
     1261 aaacccatcg gagggaatat tatcgcacat gcatcgacaa ctcgcttata tctgcgtaag
     1321 ggccgtggag aaacacgcat ctgcaaaatc tatgactccc catgcctgcc ggaggcggaa
     1381 gccatgttcg caattaacgc agatggcgta ggggacgcca aagattgatg actggtacac
     1441 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     1501 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1561 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1621 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     1681 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     1741 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1801 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1861 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1921 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1981 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2341 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     2401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     2641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3901 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg
     3961 atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     4021 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     4081 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     4141 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     4201 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     4261 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     4321 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggact
     4381 atttacggta aactgcccac ttggcagtac atcaagtgta tc
//
