LOCUS pT3TS-hypa-nCas9 7486 bp DNA circular 8-APR-2019 DEFINITION pT3TS-nCas9n. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11918371| COMMENT VNTDBDATE|-12761982| COMMENT LSOWNER| COMMENT VNTNAME|pT3TS-hypa-nCas9n-pA| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| FEATURES Location/Qualifiers source 1..7486 /organism="pT3TS-nCas9n" /mol_type="other DNA" /vntifkey="98" promoter 1..20 /vntifkey="30" /label=T3_promoter CDS 108..4286 /vntifkey="4" /label=nCas9n misc_feature 120..140 /vntifkey="21" /label=SV40\NLS misc_feature 120..143 /vntifkey="21" /label=SV40\NLS misc_feature 126..140 /vntifkey="21" /label=NLS promoter complement(4676..4694) /vntifkey="30" /label=T7_promoter misc_feature 4698..4853 /vntifkey="21" /label=lacZ_a promoter complement(4701..4717) /vntifkey="30" /label=M13_forward20_primer misc_feature complement(4710..4732) /vntifkey="21" /label=M13_pUC_fwd_primer rep_origin complement(4990..5296) /vntifkey="33" /label=f1_origin promoter 5375..5403 /vntifkey="30" /label=AmpR_promoter gene 5445..6305 /gene="Ampicillin" /vntifkey="60" CDS 5445..6305 /vntifkey="4" /label=ORF\frame\3 rep_origin 6460..7079 /vntifkey="33" /label=pBR322_origin promoter 7388..7417 /vntifkey="30" /label=lac_promoter misc_feature 7431..7453 /vntifkey="21" /label=M13_pUC_rev_primer promoter 7452..7470 /vntifkey="30" /label=M13_reverse_primer misc_feature 4293..4442 /vntifkey="21" /label=A69-polyA misc_feature 2220..2240 /vntifkey="21" /label=hypaCas9_mutated_region misc_feature 2220..2222 /vntifkey="21" /label=N692A misc_feature 2226..2228 /vntifkey="21" /label=M694A misc_feature 2229..2231 /vntifkey="21" /label=Q695A misc_feature 2238..2240 /vntifkey="21" /label=H698A misc_feature 147..4283 /vntifkey="21" /label=Cas9_CDS misc_feature 3546..3548 /vntifkey="21" /label=D1134 misc_feature 3795..3797 /vntifkey="21" /label=G1217 misc_feature 4146..4148 /vntifkey="21" /label=R1334 misc_feature 4152..4154 /vntifkey="21" /label=T1336 BASE COUNT 2264 a 1716 c 1867 g 1639 t ORIGIN 1 aattaaccct cactaaaggg aacaaaagct ggagctaagc ttgcttgttc tttttgcaga 61 agctcagaat aaacgctcaa ctttggcaga tctaactcga cgccaccatg gcttctccac 121 ctaagaagaa gagaaaggtg ggaagcatgg ataagaagta tagcatcggc ctggatattg 181 gaactaactc cgtgggttgg gcagtgatta cagacgacta caaggtccct agcaagaaat 241 ttaaggtgct gggtaacacc gacaggcaca gcatcaagaa aaatctgatt ggagccctgc 301 tgttcggttc tggagagact gccgaagcaa cacgcctgaa aagaacagca agaaggcgct 361 ataccagaag gaagaataga atctgttacc tgcaggagat tttctctaac gaaatggcta 421 aggtggacga ttcattcttt cataggctgg aggaaagttt cctggtcgag gaagataaga 481 aacacgagcg ccatcctatc tttggaaaca ttgtggacga ggtcgcctat cacgaaaaat 541 acccaaccat ctatcatctg cgcaagaaac tggctgactc tactgataaa gccgacctga 601 gactgatcta tctggctctg gcccacatga ttaagttcag gggtcatttt ctgatcgagg 661 gcgatctgaa ccccgacaat tccgatgtgg acaagctgtt catccagctg gtccagattt 721 acaatcagct gtttgaggaa aaccctatta atgcttccag agtggacgca aaagctatcc 781 tgtcagccag gctgtccaag tcacgcagac tggagaacct gattgcacag ctgcccggag 841 aaaagaggaa cggtctgttt ggaaatctga tcgctctgag tctgggcctg actcctaact 901 tcaaaagcaa ttttgatctg gctgaggacg ccaaactgca gctgtcaaag gacacatatg 961 acgatgacct ggataacctg ctggcacaga tcggagatca gtacgctgac ctgttcctgg 1021 ctgccaaaaa tctgtccgac gcaatcctgc tgtcagatat tctgagagtg aacagcgaga 1081 ttacaaaagc tcctctgagt gccagcatga tcaagagata tgacgagcac catcaggatc 1141 tgaccctgct gaaggctctg gtcaggcagc agctgccaga gaagtacaag gaaattttct 1201 ttgatcagtc caagaacggc tacgccggtt atatcgacgg aggcgcatca caggaggaat 1261 tctacaagtt tatcaaacct attctggaga agatggacgg aactgaggaa ctgctggtga 1321 aactgaatag agaggacctg ctgaggaagc agcgcacatt tgataacggt tccatcccac 1381 accagattca tctgggagag ctgcacgcta tcctgaggcg ccaggaagac ttctacccct 1441 ttctgaaaga taaccgcgag aagatcgaaa aaattctgac cttcagaatc ccttactatg 1501 tgggtccact ggctcgcgga aacagcagat ttgcctggat gactcgcaaa tccgaggaaa 1561 ccattactcc ttggaacttc gaggaagtgg tcgataaggg cgcctctgca cagtccttca 1621 tcgagagaat gactaatttt gacaaaaacc tgcccaatga gaaagtgctg cctaagcact 1681 ccctgctgta cgagtatttc actgtctata acgaactgac aaaggtgaaa tacgtcaccg 1741 agggcatgag aaagccagcc ttcctgtcag gagagcagaa gaaagcaatc gtggatctgc 1801 tgtttaaaac caacaggaaa gtgactgtca agcagctgaa ggaggactac ttcaagaaaa 1861 ttgaatgctt cgattccgtg gagatcagcg gagtcgaaga cagatttaac gcaagcctgg 1921 gcgcttacca cgatctgctg aagatcatta aggataaaga cttcctggac aacgaggaaa 1981 atgaggatat cctggaagac attgtgctga cactgaccct gtttgaggac agaggaatga 2041 tcgaggaaag actgaaaacc tatgctcatc tgttcgatga caaggtgatg aaacagctga 2101 agagaaggcg ctacactggc tggggtagac tgagcaggaa gctgatcaac ggcattaggg 2161 ataaacagtc aggaaagaca atcctggact ttctgaaaag tgatggcttc gccaaccgcg 2221 cttttgcggc actgattgcc gatgacagtc tgaccttcaa agaggacatc cagaaggctc 2281 aggtgtctgg acagggccac tccctgcatg agcagattgc aaacctggct ggaagcccag 2341 ccatcaagaa aggcattctg cagacagtga aaatcgtcga tgagctggtg aaagtcatgg 2401 gccataagcc cgaaaacatc gtgattgaga tggctcgcga aaatcagaca acccagaagg 2461 gtcagaagaa cagtagagag aggatgaaaa gaatcgagga aggcattaag gagctgggta 2521 gccagatcct gaaagagcac ccagtggaaa acacacagct gcagaatgag aagctgtatc 2581 tgtactatct gcagaatgga agagatatgt acgtggacca ggagctggat attaacaggc 2641 tgtctgatta cgacgtggat catatcgtcc cccagagttt catcaaagat gacagcattg 2701 acaacaaggt gctgaccagg tccgacaaaa acagaggaaa atcagataat gtccctagtg 2761 aggaagtggt caagaaaatg aagaactact ggagacagct gctgaatgcc aaactgatca 2821 ctcagaggaa gtttgataac ctgacaaaag cagagcgcgg tggactgtca gaactggaca 2881 aagctggatt catcaagagg cagctggtgg aaacacgcca gatcactaaa cacgtcgcac 2941 agattctgga tagtcgcatg aacacaaagt acgatgagaa tgacaaactg atcagagaag 3001 tgaaggtcat taccctgaag agtaaactgg tcagcgactt taggaaagat ttccagtttt 3061 ataaggtccg cgagattaac aattatcacc atgcccatga cgcatacctg aacgccgtgg 3121 tcggtaccgc actgatcaag aaatacccaa aactggagag cgaattcgtg tacggagact 3181 ataaggtgta cgatgtcaga aaaatgatcg ccaagtccga gcaggaaatt ggaaaagcta 3241 ctgccaagta tttcttttac tcaaacatca tgaatttctt taagacagag atcaccctgg 3301 ccaatggaga aatccgcaaa aggcccctga ttgagacaaa cggagagaca ggcgaaatcg 3361 tgtgggacaa aggcagagat tttgcaaccg tgaggaaggt cctgagcatg cctcaagtga 3421 atatcgtcaa gaaaactgag gtgcagacag gcggtttctc aaaagaaagt attctgccaa 3481 aacgcaactc tgataagctg atcgctagaa agaaagactg ggaccctaag aagtatggag 3541 gctttgactc tcccactgtg gcatactccg tcctggtggt cgctaaggtg gagaagggca 3601 aaagcaagaa actgaaatct gtcaaggagc tgctgggtat cacaattatg gagagaagct 3661 ctttcgagaa gaacccaatc gattttctgg aggccaaagg ttataaggaa gtgaagaaag 3721 acctgatcat taaactgccc aagtacagtc tgtttgagct ggaaaacggc aggaaacgca 3781 tgctggcaag cgctggagag ctgcagaaag gcaatgaact ggccctgcct tctaagtacg 3841 tgaacttcct gtatctggca agccactacg agaagctgaa aggatctcca gaggataacg 3901 aacagaaaca gctgtttgtg gagcagcaca agcattatct ggacgagatc attgaacaga 3961 ttagcgagtt ctctaaaaga gtgatcctgg ccgacgcaaa tctggataag gtcctgtctg 4021 cttacaacaa acacagagat aagcccatca gggagcaggc cgaaaatatc attcatctgt 4081 tcactctgac aaacctgggc gcacctgcag ctttcaagta cttcgacact acaatcgata 4141 gaaagaggta cacctccact aaggaggtgc tggacgctac actgatccat cagagtatta 4201 ccggcctgta cgaaacaagg attgacctgt ctcagctggg tggcgactca ccggtgagat 4261 ctcctaagaa gaagagaaag gtgtgaccgc gggcaccaaa gaacctgtaa acgttatctt 4321 tttaaattga atgtgcacaa ataaaagttt ggaaaagaaa aaaaaaaaaa aaaaaaaaaa 4381 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa actcgaggta gcatgctcta 4441 gaccgcggat ctggttacca ctaaaccagc ctcaagaaca cccgaatgga gtctctaagc 4501 tacataatac caacttacac tttacaaaat gttgtccccc aaaatgtagc cattcgtatc 4561 tgctcctaat aaaaagaaag tttcttcaca ttctaaaaaa aaaaaaaaaa aaaaaaaaaa 4621 aaaccccccc ccccccctgc agggccaagt cggcctctag aggtacccaa ttcgccctat 4681 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 4741 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 4801 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa 4861 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 4921 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 4981 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 5041 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 5101 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 5161 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 5221 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 5281 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc 5341 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 5401 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 5461 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 5521 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 5581 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 5641 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 5701 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 5761 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 5821 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 5881 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 5941 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 6001 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 6061 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 6121 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 6181 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 6241 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 6301 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 6361 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 6421 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 6481 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 6541 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 6601 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 6661 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 6721 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 6781 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 6841 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 6901 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 6961 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 7021 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 7081 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 7141 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 7201 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 7261 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 7321 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 7381 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 7441 aatttcacac aggaaacagc tatgaccatg attacgccaa gctcga //