LOCUS       Exported                8468 bp ds-DNA     circular SYN 14-FEB-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 4, 2019 from SnapGene 4.3.6
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
                     /note="color: #80ffff"
     misc_feature    851..1558
                     /locus_tag="yeast optimized mneongreen"
                     /label=yeast optimized mneongreen
                     /note="color: #00ff00"
     misc_feature    1559..1639
                     /locus_tag="T2A"
                     /label=T2A
                     /note="color: #008000"
     misc_feature    1640..2350
                     /locus_tag="mCherry"
                     /label=mCherry
                     /note="color: #fa77c8"
     misc_feature    4723..5522
                     /locus_tag="URA3"
                     /label=URA3
                     /note="color: #00ff00"
     misc_feature    5954..6814
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
                     /note="color: #00ff00"
     misc_feature    7897..7926
                     /locus_tag="lac promotor"
                     /label=lac promotor
                     /note="color: #ff0000"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc actagtaagc
      841 ttttaattaa atggtctcta agggtgaaga agataacatg gcttctttgc cagccaccca
      901 cgagttgcac atcttcggtt ccattaacgg tgtcgacttc gatatggtcg gtcaaggtac
      961 cggtaaccca aacgacggtt acgaagaatt gaacttgaag tctaccaagg gcgacttgca
     1021 gttctcccct tggattttgg ttccacacat cggttacggt ttccaccaat acttgccata
     1081 cccagacggt atgtccccat tccaagccgc catggttgac ggttctggtt accaagtcca
     1141 cagaaccatg caattcgaag acggtgcttc cttgaccgtg aactacagat acacctacga
     1201 aggttctcac atcaagggcg aagcccaagt taagggtacc ggtttccctg ctgacggtcc
     1261 agtcatgact aactccttga ccgctgctga ctggtgtcgt tccaagaaga cctacccaaa
     1321 cgataagacc atcatttcca ctttcaagtg gtcctacacc accggtaacg gcaagagata
     1381 ccgctctacc gctcgtacca cctacacctt cgccaagcca atggctgcca actacttgaa
     1441 gaaccagcct atgtacgtct tccgtaagac cgagctgaag cattccaaga ctgaattgaa
     1501 cttcaaggaa tggcaaaagg ctttcaccga cgtcatgggt atggacgaat tgtacaagtc
     1561 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctgg
     1621 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     1681 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     1741 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1801 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     1861 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     1921 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     1981 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     2041 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     2101 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     2161 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     2221 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     2281 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     2341 gtacaagtag cggccgcacc cagctttctt gtacaaagtg gtgtagttaa ttcatgatgg
     2401 ccgctgcagg tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
     2461 ttacgcgcgg atccagcttt ggacttcttc gccagaggtt tggtcaagtc tccaatcaag
     2521 gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt
     2581 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
     2641 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     2701 aaacganaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     2761 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccaattca ctggccgtcg
     2821 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2881 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2941 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     3001 gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa ttttgaacat
     3061 ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata
     3121 gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct
     3181 attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca
     3241 cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc
     3301 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     3361 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
     3421 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     3481 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
     3541 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
     3601 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
     3661 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
     3721 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
     3781 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
     3841 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
     3901 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
     3961 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
     4021 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
     4081 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
     4141 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
     4201 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag aataggaact
     4261 tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc
     4321 tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata
     4381 tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct
     4441 atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg
     4501 gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat
     4561 tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga tccgatgata
     4621 agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc tctaatttgt
     4681 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     4741 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     4801 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     4861 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     4921 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     4981 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     5041 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     5101 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     5161 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     5221 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     5281 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     5341 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     5401 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     5461 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     5521 atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa gaatactggg
     5581 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     5641 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
     5701 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa
     5761 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5821 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5881 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5941 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6001 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6061 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6121 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6181 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6241 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6301 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6361 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6421 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6481 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6541 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6601 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6661 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6721 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6781 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6841 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6901 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7141 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7381 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7621 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7681 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7741 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7801 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7861 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7921 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7981 ttacgccaag cttaccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     8041 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     8101 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     8161 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     8221 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     8281 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     8341 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     8401 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     8461 ccattcgc
//
