LOCUS       Exported                5609 bp ds-DNA     circular SYN 24-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Tivan27
  TITLE     Direct Submission
  JOURNAL   Exported Apr 5, 2019 from SnapGene Viewer 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     65..82
                     /label=OS22
     primer_bind     114..135
                     /label=OS21
     primer_bind     126..145
                     /label=OS26
     primer_bind     137..151
                     /label=MM154_ITR-MluI_fw_seq
     primer_bind     150..181
                     /label=C114
     primer_bind     150..181
                     /label=C114_CKIIa(0.4)-fw
     promoter        156..518
                     /label=a-CaMKII(0.4)
                     /note="short version of alpha-CamKinase II promoter (0.4kb)
                     specific for excitatory neurons in neocortex"
     primer_bind     156..181
                     /label=MM147_MluI-Nhe-CKIIa_fw
     primer_bind     156..181
                     /label=MM137_MluI-Nhe-CKIIa_fw
     primer_bind     383..401
                     /label=S004
                     /note="F' sequencing primer starting at end of CamKII 
                     promoter- Shiri ShS1"
     primer_bind     complement(501..518)
                     /label=MM185_AgeI-CKII_mScarlet_rev
     primer_bind     complement(527..550)
                     /label=MM68_Proj2_FS-Fix_Rev (not sorted by number)
     primer_bind     complement(527..547)
                     /label=BeGC_trun_rev_(454)
     primer_bind     533..547
                     /label=MM194_BamHI_SthK_fw
     CDS             545..1534
                     /codon_start=1
                     /product="mosquito OPN3 
                      Anopheles stephensi (Indo-Pakistan malaria mosquito) 
                       http://www.uniprot.org/uniprot/M5AB93"
                     /label=MosOPN3
                     /translation="MYDAPNDVASSVADYEDLMAPWAYNAAAITLFFIGFFGFFLNLFV
                     IALMSKDMQLWTPMNIILFNLVCSDFSVSIIGNPLTLTSAISHRWIFGRTLCVAYGFFM
                     SLLGITSITTLTVLSYERYCLISRPFSSRNLSRKGAFLAIFFIWGYSFALTSPPLFGWG
                     AYVQEAANISCSVNWESQTKNATTYIIFLFVFGLVVPLIVIVYSYTNIIVYMRRNSARV
                     GRINRAEQRVTSMVAVMIVAFMVAWTPYAIFALIEQFGPPELIGPGLAVLPALIAKSSI
                     CYNPIIYVGMNTQFRAAFTRVRNKGGVPTADQNTTTMQRELTKSSRDMV"
     CDS             1535..1561
                     /codon_start=1
                     /label=bRho-M4
                     /translation="TETSQVAPA"
     primer_bind     complement(1560..1585)
                     /label=MM213_MosOPN3rev_AgeI
     CDS             1562..1579
                     /codon_start=1
                     /label=Linker
                     /translation="PRARDP"
     primer_bind     1579..1667
                     /label=MM210_AgeI-ts-mScarlet_fw
     CDS             1586..1645
                     /codon_start=1
                     /product="membrane trafficking sequence Kir2.1"
                     /label=ts
                     /translation="KSRITSEGEYIPLDQIDINV"
     primer_bind     1586..1607
                     /label=MM98_AA-ts_for
     primer_bind     1586..1602
                     /label=MM167-ts-Fwd
     primer_bind     1588..1606
                     /label=seq_ts_for_(391)
     primer_bind     complement(1622..1648)
                     /label=P2A_C1V1_rev_(225) (2)
     primer_bind     complement(1622..1648)
                     /label=P2A_C1V1_rev_(225)
     primer_bind     complement(1622..1646)
                     /label=OC92
     primer_bind     complement(1625..1648)
                     /label=MM120_ts_rev_seq
     primer_bind     complement(1627..1667)
                     /label=YP27_TS_rev
     primer_bind     complement(1633..1648)
                     /label=ts_rev_(372)
     primer_bind     1645..1666
                     /label=MP_P2A_NLS_mSca_f_(635)
     primer_bind     1646..1666
                     /label=mGFP_o_ChRI_for_(621)
     primer_bind     1648..1667
                     /label=MM80_AscII-TagRFP-T-fw (not sorted by number)
     CDS             1649..2344
                     /codon_start=1
                     /product="https://www.ncbi.nlm.nih.gov/nuccore/KY021423
                     mScarlet: a bright monomeric red fluorescent protein for 
                     cellular imaging
                     Nat. Methods (2016)
                     http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmet
                     h.4074.html"
                     /label=mScarlet
                     /note="https://www.ncbi.nlm.nih.gov/nuccore/KY021423"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     1649..1667
                     /label=MM173_BamHI-Kozak-AgeI-mScarlet_Fw_fix
     primer_bind     1649..1667
                     /label=YP23_mScarlet_fwd
     primer_bind     1649..1667
                     /label=MM172_BamHI-Kozak-AgeI-mScarlet_Fw
     primer_bind     1649..1667
                     /label=hChR2_o_TaqRFP_for_(601)
     primer_bind     1649..1667
                     /label=C094
     primer_bind     1649..1667
                     /label=MM125_AscI-Kozak-XFP-fw.
     primer_bind     complement(1649..1667)
                     /label=N_eYFP_rev_(239_3)
     primer_bind     1649..1667
                     /label=YP28_mScarlet_fwd2
     primer_bind     1649..1663
                     /label=eYFP_for_(43)
     primer_bind     1649..1661
                     /label=MP_NheI_mScarlet_for_(652)
     primer_bind     complement(1652..1667)
                     /label=15aa_eYFP_rev_o_LOV_(244)
     primer_bind     complement(1653..1667)
                     /label=C25_VVD_rev(+EYFPprim_fwd)
     primer_bind     complement(1653..1667)
                     /label=C025
     primer_bind     1666..1693
                     /label=MP_AscI_mSarlet_for_(684)
     primer_bind     1666..1693
                     /label=MP_dt_mScarlet_for_(658)
     primer_bind     complement(1705..1722)
                     /label=OS14
     primer_bind     complement(1717..1736)
                     /label=S1_mRFP_seq_upstream
     primer_bind     complement(1717..1736)
                     /label=S01
     primer_bind     complement(1717..1736)
                     /label=S001
     primer_bind     complement(1751..1760)
                     /label=AscI_mRFP_rev_(251)
     primer_bind     complement(1751..1760)
                     /label=EcoRI_RFP_rev_(198)
     primer_bind     complement(2272..2293)
                     /label=MP_mScarlet_seq_for_(664)
     primer_bind     2272..2293
                     /label=MP_mScarlet_seq_rev_(665)
     primer_bind     complement(2303..2327)
                     /label=AscI_mRFP_rev_(251)
     primer_bind     complement(2303..2327)
                     /label=EcoRI_RFP_rev_(198)
     primer_bind     complement(2308..2327)
                     /label=linker_mRFP_rev_(207)
     primer_bind     complement(2320..2345)
                     /label=OC109
     primer_bind     complement(2320..2345)
                     /label=MP_AscI_mSarlet_for_(659)
     primer_bind     complement(2320..2345)
                     /label=MP_AscI_mSca_for_(654)
     primer_bind     complement(2322..2401)
                     /label=pL_o_mCherry_rev_(256)
     primer_bind     complement(2322..2345)
                     /label=OC110
     primer_bind     complement(2322..2345)
                     /label=OC48
     primer_bind     complement(2323..2377)
                     /label=C99_mC-Rv_BB-Fw
     primer_bind     complement(2323..2377)
                     /label=C099
     primer_bind     complement(2323..2345)
                     /label=OC112new
     primer_bind     complement(2323..2345)
                     /label=OC142
     primer_bind     complement(2323..2345)
                     /label=OC145
     primer_bind     complement(2323..2345)
                     /label=EcoRI_pHTomato_rev_(401)
     primer_bind     complement(2323..2345)
                     /label=OC115new
     primer_bind     complement(2323..2345)
                     /label=OC46
     primer_bind     complement(2323..2345)
                     /label=OC97
     primer_bind     complement(2323..2345)
                     /label=OC108
     primer_bind     complement(2323..2345)
                     /label=OC117
     primer_bind     complement(2323..2345)
                     /label=BspEI_eYFP_rev_(487)
     primer_bind     complement(2323..2344)
                     /label=WPRE_pHTomato_rev_(360)
     primer_bind     complement(2323..2344)
                     /label=YP29_mScarlet_rev2
     primer_bind     complement(2324..2419)
                     /label=MM145_RFP-AscI_WPRE_rev
     primer_bind     complement(2324..2345)
                     /label=Asc_eYFP_rev_(223)
     primer_bind     complement(2324..2345)
                     /label=Ecor_LOVtoxin_rev_(234)
     primer_bind     complement(2324..2345)
                     /label=MfeI_LOVtoxin_rev_(254)
     primer_bind     complement(2324..2345)
                     /label=NheI_eYFP_STOP_rev_(145)
     primer_bind     complement(2324..2345)
                     /label=EcoRI_eYFP_rev_(34)
     primer_bind     complement(2324..2345)
                     /label=NheI_mCherry_for_(377)
     primer_bind     complement(2324..2345)
                     /label=OC114
     primer_bind     complement(2324..2345)
                     /label=OC111
     primer_bind     complement(2324..2345)
                     /label=OC148
     primer_bind     complement(2324..2345)
                     /label=YP37_mCyRFP1_rev
     primer_bind     complement(2324..2344)
                     /label=MP_AscI_Con_GP2A_r_(634)
     primer_bind     complement(2324..2344)
                     /label=C2_pmEYFP_rev(+pm+EcoRI)
     primer_bind     complement(2324..2344)
                     /label=C002
     primer_bind     complement(2324..2344)
                     /label=OC04
     primer_bind     complement(2324..2344)
                     /label=eT2A_mTurq_rev_(555)
     primer_bind     complement(2324..2344)
                     /label=MM144_P2A-XFP_rev
     primer_bind     complement(2324..2344)
                     /label=p2a_o_TagRFP_rev_(602)
     primer_bind     complement(2324..2344)
                     /label=AscI_eYFP_rev_(270)
     primer_bind     complement(2324..2344)
                     /label=YP11_mCyRFP1_rev
     primer_bind     complement(2324..2344)
                     /label=C14_EYFP_rev(+BsiWI)
     primer_bind     complement(2324..2344)
                     /label=C014
     primer_bind     complement(2324..2344)
                     /label=C16_mCherry_rev(+BsiWI)
     primer_bind     complement(2324..2344)
                     /label=C016
     primer_bind     complement(2324..2344)
                     /label=MP_eGFP_o_beta_rev_(646)
     primer_bind     complement(2324..2344)
                     /label=MP_AscI_Sca_noSTOP_rev_(682)
     primer_bind     complement(2324..2344)
                     /label=eYFP_AgeI_rev_(1)
     primer_bind     complement(2324..2344)
                     /label=C57_EYFP_rev
     primer_bind     complement(2324..2344)
                     /label=C057_missing_seeYP29
     primer_bind     2324..2344
                     /label=MM131_eYFP_end_fw
     primer_bind     complement(2325..2377)
                     /label=MM211_EcoRI_ER-mScarlet_rev
     primer_bind     complement(2325..2345)
                     /label=OC143
     primer_bind     complement(2325..2345)
                     /label=C122_dTom_rev(+EcoRI)
     primer_bind     complement(2325..2345)
                     /label=OC150
     primer_bind     complement(2325..2345)
                     /label=MM193_mCherry_HindIII_rev
     primer_bind     complement(2325..2344)
                     /label=m_turq_o_ChRI_rev_(568)
     primer_bind     complement(2327..2345)
                     /label=YP24_mScarlet_rev
     primer_bind     complement(2327..2345)
                     /label=BamHI_eyFP_rev_(51)
     primer_bind     complement(2329..2345)
                     /label=C1_EYFP_rev(+EcoRI)
     primer_bind     complement(2329..2345)
                     /label=C001
     primer_bind     complement(2341..2395)
                     /label=pL_Syn_o_Arch_rev_(169)
     primer_bind     complement(2341..2366)
                     /label=EcoR_er_eYFP_rev_(230)
     primer_bind     complement(2341..2366)
                     /label=EcoR_er_eYFP_rev_(230) (2)
     primer_bind     complement(2341..2365)
                     /label=P2A_o_Arch_rev_(161)
     primer_bind     complement(2344..2368)
                     /label=NheI_mCherry_rev_(370)
     primer_bind     complement(2345..2368)
                     /label=AscI_er_rev_(538)
     CDS             2345..2365
                     /codon_start=1
                     /product="ER export signal Kir2.1"
                     /label=ER
                     /translation="FCYENEV"
     primer_bind     complement(2347..2377)
                     /label=OC79
     primer_bind     complement(2347..2368)
                     /label=NheI-mCherry-rev_(MP300)
     primer_bind     complement(2347..2368)
                     /label=BspeEI_er_rev_(586)
     primer_bind     2371..2411
                     /label=MM148_MluI-EcoRI-WPRE
     misc_feature    2384..2787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     2384..2419
                     /label=MM146_WPRE_fw
     primer_bind     complement(2438..2459)
                     /label=OS07
                     /note="Sequences back from WPRE on Lenti vectors"
     primer_bind     2457..2478
                     /label=OS68
     primer_bind     complement(2459..2478)
                     /label=G06 Ai9 mut R'
     primer_bind     complement(2459..2478)
                     /label=G35 Ai32-mut-rev
     primer_bind     2651..2670
                     /label=SqP for pCL-CAG-EGFP-WPRE_11
     primer_bind     complement(2767..2785)
                     /label=seq_WPRE_upstream_rev_(347)
     polyA_signal    2794..2842
                     /note="synthetic polyadenylation signal"
     primer_bind     2801..2820
                     /label=MM95_Test_RF_pr30 (not sorted by number)
     primer_bind     complement(2816..2846)
                     /label=MM133_minPolyA_NheI
     primer_bind     complement(2816..2842)
                     /label=695_minPolyA_Mlu_MP
     primer_bind     complement(2816..2842)
                     /label=MP_MluI_polyA_rev_(667)
     primer_bind     complement(2823..2842)
                     /label=seq_NaN_(632)
     primer_bind     complement(2844..2862)
                     /label=MP_aPAAA_rev_(670)
     primer_bind     complement(2862..2877)
                     /label=MM153_ITR_rev_seq
     primer_bind     complement(2868..2887)
                     /label=OS26
     repeat_region   2872..3012
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(2878..2899)
                     /label=OS21
     primer_bind     complement(2931..2948)
                     /label=OS22
     rep_origin      3087..3542
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3093..3112)
                     /label=MM119_Sequencing_ITR2
     primer_bind     complement(3732..3751)
                     /label=SqP for pRS315 (LEU)_01
     primer_bind     complement(3738..3757)
                     /label=SqP for pRS315 (LEU)_11
     promoter        3824..3928
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3824..3843)
                     /label=SqP for pRS315 (LEU)_10
     CDS             3929..4789
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4425..4444)
                     /label=SqP for pRS315 (LEU)_09
     rep_origin      4960..5548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5008..5022
                     /label=SecSig_o_Bgtx_for_(245)
     primer_bind     complement(5026..5045)
                     /label=SqP for pRS315 (LEU)_08
     primer_bind     5485..5504
                     /label=MM118_Sequencing_ITR1
     primer_bind     5529..5552
                     /label=OC57a
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcttgt ggactaagtt tgttcgcatc
      181 cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc
      241 ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag
      301 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
      361 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
      421 gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga
      481 gcacgggcag gcgagtggcc cctagttctg ggggcagctc tagagcggta ccggatccgc
      541 caccatgtac gacgccccca acgacgtggc cagcagcgtg gccgactacg aggacctgat
      601 ggccccctgg gcctacaacg ccgccgccat caccctgttc ttcatcggct tcttcggctt
      661 cttcctgaac ctgttcgtga tcgccctgat gagcaaggac atgcagctgt ggacccccat
      721 gaacatcatc ctgttcaacc tggtgtgcag cgacttcagc gtgagcatca tcggcaaccc
      781 cctgaccctg accagcgcca tcagccacag gtggatcttc ggcaggaccc tgtgcgtggc
      841 ctacggcttc ttcatgagcc tgctgggcat caccagcatc accaccctga ccgtgctgag
      901 ctacgagagg tactgcctga tcagcaggcc cttcagcagc aggaacctga gcaggaaggg
      961 cgccttcctg gccatcttct tcatctgggg ctacagcttc gccctgacca gcccccccct
     1021 gttcggctgg ggcgcctacg tgcaggaggc cgccaacatc agctgcagcg tgaactggga
     1081 gagccagacc aagaacgcca ccacctacat catcttcctg ttcgtgttcg gcctggtggt
     1141 gcccctgatc gtgatcgtgt acagctacac caacatcatc gtgtacatga ggaggaacag
     1201 cgccagggtg ggcaggatca acagggccga gcagagggtg accagcatgg tggccgtgat
     1261 gatcgtggcc ttcatggtgg cctggacccc ctacgccatc ttcgccctga tcgagcagtt
     1321 cggccccccc gagctgatcg gccctggact ggctgtgctg cctgctctga tcgccaagag
     1381 cagcatctgc tacaacccca tcatctacgt gggcatgaac acccagttca gggccgcctt
     1441 caccagggtg aggaacaagg gcggcgtgcc caccgccgac cagaacacca ccaccatgca
     1501 gagggagctg accaagagca gcagggacat ggtgaccgag accagccagg tggctcctgc
     1561 tccgcgagcc cgagatccaa ccggtaagag caggatcacc agcgagggcg agtacatccc
     1621 cctggaccag atcgacatca acgtggtgat ggtgagcaag ggcgaggcag tgatcaagga
     1681 gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac ggccacgagt tcgagatcga
     1741 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1801 gggtggcccc ctgcccttct cctgggacat cctgtcccct cagttcatgt acggctccag
     1861 ggccttcacc aagcaccccg ccgacatccc cgactactat aagcagtcct tccccgaggg
     1921 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gccgtgaccg tgacccagga
     1981 cacctccctg gaggacggca ccctgatcta caaggtgaag ctccgcggca ccaacttccc
     2041 tcctgacggc cccgtaatgc agaagaagac aatgggctgg gaagcgtcca ccgagcggtt
     2101 gtaccccgag gacggcgtgc tgaagggcga cattaagatg gccctgcgcc tgaaggacgg
     2161 cggacgctac ctggcggact tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc
     2221 cggcgcctac aacgtcgacc gcaagttgga catcacctcc cacaacgagg actacaccgt
     2281 ggtggaacag tacgaacgct ccgagggccg ccactccacc ggcggcatgg acgagctgta
     2341 caagttctgc tacgagaacg aggtgtaact agaattcaag cttaatcaac ctctggatta
     2401 caaaatttgt gaaagattga ctgatattct taactatgtt gctcctttta cgctgtgtgg
     2461 atatgctgct ttaatgcctc tgtatcatgc tattgcttcc cgtatggctt tcgttttctc
     2521 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtccgtca
     2581 acgtggcgtg gtgtgctctg tgtttgctga cgcaaccccc actggctggg gcattgccac
     2641 cacctgtcaa ctcctttctg ggactttcgc tttccccctc ccgatcgcca cggcagaact
     2701 catcgccgcc tgccttgccc gctgctggac aggggctagg ttgctgggca ctgataattc
     2761 cgtggtgttg tcggggaagc tgacgtcagc gctaataaaa tcgctatcca tcgaagatgg
     2821 atgtgtgttg gttttttgtg tgggtaaccc acgtgcggac cgagcggccg caggaacccc
     2881 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     2941 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     3001 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3061 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     3121 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3181 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3241 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3301 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3361 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3421 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3481 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     3541 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3601 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3661 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3721 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3781 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3841 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3901 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3961 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4021 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4081 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4141 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4201 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4261 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4321 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4381 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4441 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4501 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4561 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4621 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4681 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4741 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4801 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4861 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4981 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5041 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5101 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5161 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5221 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5281 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5341 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5401 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5461 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5521 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5581 ggccttttgc tggccttttg ctcacatgt
//
